DBX2 (NM_001004329) Human Tagged ORF Clone

CAT#: RG224644

DBX2 (GFP-tagged) - Human developing brain homeobox 2 (DBX2)

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: DBX2
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG224644 representing NM_001004329
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCTCCCCAGCGCGGTCGCAGCCCACGCCGGTGCGTACTGGGACGTTGTGGCTTCCTCCGCGCTCCTCA
  ACCTCCCCGCTGCGCCCGGCTTTGGCAACCTGGGCAAGAGTTTCCTGATCGAGAATTTGCTGCGGGTCGG
  GGGCGCCCCAACGCCCAGGCTGCAGCCGCCCGCGCCCCACGACCCGGCGACCGCCCTGGCCACCGCGGGC
  GCGCAGCTCCGGCCCCTGCCTGCTAGCCCAGTTCCCCTAAAGCTGTGCCCCGCAGCCGAACAAGTTAGCC
  CCGCCGGGGCGCCCTACGGAACGCGGTGGGCTTTTCAAGTGCTCAGTCCCTCTGCGGACAGTGCGAGGCT
  GCCGGGCCGGGCTCCGGGGGACCGAGACTGTACCTTCCAGCCTTCAGCGCCAGCACCTTCCAAACCTTTC
  CTTCTGAGCACCCCGCCATTCTACTCGGCGTGCTGCGGTGGGTCCTGTCGGCGCCCTGCATCCTCCACTG
  CTTTTCCAAGAGAAGAGAGCGTGCTGCCTCTCCTGACACAGGACTCTAATTCCAAAGCTCGGAGGGGCAT
  TTTAAGAAGAGCTGTCTTTTCTGAGGACCAGAGAAAGGCTCTGGAGAAAATGTTTCAGAAACAGAAATAT
  ATCAGCAAAACAGACCGAAAGAAACTTGCCATCAACTTGGGACTAAAGGAATCACAGGTGAAAATTTGGT
  TTCAGAACAGGAGGATGAAATGGCGGAATTCCAAAGAAAAGGAAGTGCTTTCCAACAGGTGTATCCAAGA
  AGTAGGTCTTCAAGAGGATCCCCTCTCACGGTCTGCTCTGGGTTTCCCTTCTCCATGTCCTTCAATATGG
  GACGTCCCCCAACAGCACTCAAGTCCAAGATGGAGGGAGAATTCTCCAGAACCTTCAGAAAGACTAATCC
  AGGAGAGTTCAGGGGCACCACCCCCAGAAGCAAATTCACTGCAAGGTGCCTTATATTTATGTTCTGAAGA
  AGAAGCTGGAAGCAAGGGTGTACTTACTGGGGCCGTC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG224644 representing NM_001004329
  Red=Cloning site Green=Tags(s)
  
  MLPSAVAAHAGAYWDVVASSALLNLPAAPGFGNLGKSFLIENLLRVGGAPTPRLQPPAPHDPATALATAG
  AQLRPLPASPVPLKLCPAAEQVSPAGAPYGTRWAFQVLSPSADSARLPGRAPGDRDCTFQPSAPAPSKPF
  LLSTPPFYSACCGGSCRRPASSTAFPREESVLPLLTQDSNSKARRGILRRAVFSEDQRKALEKMFQKQKY
  ISKTDRKKLAINLGLKESQVKIWFQNRRMKWRNSKEKEVLSNRCIQEVGLQEDPLSRSALGFPSPCPSIW
  DVPQQHSSPRWRENSPEPSERLIQESSGAPPPEANSLQGALYLCSEEEAGSKGVLTGAV
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001004329
• ORF Size: 1017 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001004329.1, NP_001004329.1
• RefSeq Size: 2722 bp
• RefSeq ORF: 1020 bp
• Locus ID: 440097
• Cytogenetics: 12q12
• Protein Families: ES Cell Differentiation/IPS