GIT1 (NM_001085454) Human Tagged ORF Clone

CAT#: RG224712

GIT1 (GFP-tagged) - Human G protein-coupled receptor kinase interacting ArfGAP 1 (GIT1), transcript variant 1

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: GIT1
• Synonyms: G protein-coupled receptor kinase-interactor 1; G protein-coupled receptor kinase interacting ArfGAP 1; G protein-coupled receptor kinase interactor 1; OTTHUMP00000163606
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG224712 representing NM_001085454
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCCCGAAAGGGGCCGCGAGCGGAGGTGTGTGCGGACTGCAGCGCCCCGGACCCTGGCTGGGCATCCA
  TCAGCAGGGGTGTGCTGGTGTGTGACGAGTGCTGCAGCGTGCACCGGAGCCTGGGACGCCACATCTCCAT
  TGTCAAGCACCTTCGCCACAGCGCCTGGCCTCCCACGCTGCTGCAGATGGTGCACACGCTTGCCAGCAAC
  GGGGCCAACTCCATCTGGGAGCACTCCCTGCTGGACCCCGCACAAGTGCAGAGCGGCCGGCGTAAAGCCA
  ACCCCCAAGACAAAGTCCACCCCATCAAGTCAGAGTTCATCAGGGCCAAGTACCAGATGCTGGCATTTGT
  GCACAAGCTTCCCTGCCGGGACGATGATGGAGTCACCGCCAAAGACCTCAGCAAGCAACTACACTCGAGC
  GTGCGGACAGGCAACCTGGAGACATGTCTGCGCCTGCTCTCCCTGGGTGCCCAGGCCAACTTCTTCCACC
  CAGAGAAGGGCACCACACCTCTGCACGTGGCTGCCAAGGCAGGACAGACACTGCAGGCCGAGCTGCTTGT
  AGTGTATGGGGCTGACCCTGGCTCCCCTGATGTTAATGGCCGCACACCCATTGACTATGCCAGGCAGGCG
  GGGCACCATGAGCTGGCGGAAAGGCTGGTTGAGTGCCAATATGAGCTCACTGACCGGCTGGCCTTCTACC
  TCTGTGGACGCAAGCCGGATCACAAGAATGGGCATTACATCATCCCACAGATGGCTGACAGATCTCGGCA
  AAAGTGCATGTCTCAGAGCCTTGACTTATCCGAATTGGCCAAAGCTGCTAAGAAGAAGCTGCAGGCGCTC
  AGCAACCGGCTTTTTGAGGAACTCGCCATGGACGTGTATGACGAGGTGGATCGAAGAGAAAATGATGCAG
  TGTGGCTGGCTACCCAAAACCACAGCACTCTGGTGACAGAGCGCAGTGCCGTGCCCTTCCTGCCTGTTAA
  CCCGGAATACTCAGCCACGCGGAATCAGGGGCGACAAAAGCTGGCCCGCTTTAATGCCCGAGAGTTTGCC
  ACCTTGATCATCGACATTCTCAGTGAGGCCAAGCGGAGACAGCAGGGCAAGAGCCTGAGCAGCCCCACAG
  ACAACCTCGAGCTGTCTCTGCGGAGCCAGAGTGACCTCGACGACCAACACGACTACGACAGCGTGGCCTC
  TGACGAGGACACAGACCAGGAGCCCCTGCGCAGCACCGGCGCCACTCGGAGCAACCGGGCCCGGAGCATG
  GACTCCTCGGACTTGTCTGACGGGGCTGTGACGCTGCAGGAGTACCTGGAGCTGAAGAAGGCCCTGGCTA
  CATCGGAGGCAAAGGTGCAGCAGCTCATGAAGGTCAACAGTAGCCTGAGCGACGAGCTCCGGAGGCTGCA
  GCGAGAGATCCACAAGCTGCAGGCGGAGAACCTGCAGCTCCGGCAGCCTCCAGGGCCGGTGCCCACACCT
  CCACTCCCCAGTGAACGGGCGGAACACACACCCATGGCGCCAGGCGGGAGCACACACCGCAGGGATCGCC
  AGGCCTTTTCCATGTATGAACCTGGCTCTGCCCTGAAGCCCTTTGGGGGCCCCCCTGGGGACGAGCTCAC
  TACGCGGCTGCAGCCTTTCCACAGCACTGAGCTAGAGGACGACGCCATCTATTCAGTGCACGTCCCTGCT
  GGCCTTTACCGGATCCGGAAAGGGGTGTCTGCCTCAGCTGTGCCCTTCACTCCCTCCTCCCCGCTGCTGT
  CCTGCTCCCAGGAGGGAAGCCGCCACACGAGCAAGCTTTCCCGCCACGGCAGTGGAGCCGACAGTGACTA
  TGAGAACACGCAAAGTGGGGACCCACTGCTGGGGCTGGAAGGGAAGAGGTTTCTAGAGCTGGGCAAAGAG
  GAAGACTTCCACCCAGAGCTGGAAAGCCTGGATGGAGACCTAGATCCTGGGCTTCCCAGCACAGAGGATG
  TCATCTTGAAGACAGAGCAGGTCACCAAGAACATTCAGGAACTGTTGCGGGCAGCCCAGGAGTTCAAGCA
  TGACAGCTTCGTGCCCTGCTCAGAGAAGATCCATTTGGCTGTGACCGAGATGGCCTCCCTCTTCCCAAAG
  AGGCCAGCCCTGGAGCCAGTGCGGAGCTCACTGCGGCTGCTCAACGCCAGCGCCTACCGGCTGCAGAGTG
  AGTGCCGGAAGACAGTGCCCCCAGAGCCCGGCGCCCCAGTGGACTTCCAGCTGCTGACTCAGCAGGTGAT
  CCAGTGCGCCTATGACATCGCCAAGGCTGCCAAGCAGCTGGTCACCATCACCACCCGAGAGAAGAAGCAG
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG224712 representing NM_001085454
  Red=Cloning site Green=Tags(s)
  
  MSRKGPRAEVCADCSAPDPGWASISRGVLVCDECCSVHRSLGRHISIVKHLRHSAWPPTLLQMVHTLASN
  GANSIWEHSLLDPAQVQSGRRKANPQDKVHPIKSEFIRAKYQMLAFVHKLPCRDDDGVTAKDLSKQLHSS
  VRTGNLETCLRLLSLGAQANFFHPEKGTTPLHVAAKAGQTLQAELLVVYGADPGSPDVNGRTPIDYARQA
  GHHELAERLVECQYELTDRLAFYLCGRKPDHKNGHYIIPQMADRSRQKCMSQSLDLSELAKAAKKKLQAL
  SNRLFEELAMDVYDEVDRRENDAVWLATQNHSTLVTERSAVPFLPVNPEYSATRNQGRQKLARFNAREFA
  TLIIDILSEAKRRQQGKSLSSPTDNLELSLRSQSDLDDQHDYDSVASDEDTDQEPLRSTGATRSNRARSM
  DSSDLSDGAVTLQEYLELKKALATSEAKVQQLMKVNSSLSDELRRLQREIHKLQAENLQLRQPPGPVPTP
  PLPSERAEHTPMAPGGSTHRRDRQAFSMYEPGSALKPFGGPPGDELTTRLQPFHSTELEDDAIYSVHVPA
  GLYRIRKGVSASAVPFTPSSPLLSCSQEGSRHTSKLSRHGSGADSDYENTQSGDPLLGLEGKRFLELGKE
  EDFHPELESLDGDLDPGLPSTEDVILKTEQVTKNIQELLRAAQEFKHDSFVPCSEKIHLAVTEMASLFPK
  RPALEPVRSSLRLLNASAYRLQSECRKTVPPEPGAPVDFQLLTQQVIQCAYDIAKAAKQLVTITTREKKQ
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001085454
• ORF Size: 2310 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_001085454.1, NP_001078923.1
• RefSeq Size: 3778
• RefSeq ORF: 2313
• Locus ID: 28964
• Protein Families: Druggable Genome
• Protein Pathways: Endocytosis, Epithelial cell signaling in Helicobacter pylori infection, Regulation of actin cytoskeleton