PCDHA10 (NM_031860) Human Tagged ORF Clone

CAT#: RG224718

PCDHA10 (GFP-tagged) - Human protocadherin alpha 10 (PCDHA10), transcript variant 3

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: PCDHA10
• Synonyms: CNR8; CNRN8; CNRS8; CRNR8; PCDH-ALPHA10
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG224718 representing NM_031860
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGTTTCCAGATGTAGCTGCCTGGGGGTCCAGTGTCTGCTGCTCTCGCTTCTTCTCCTCGCAGCCTGGG
  AGGTGGGGAGCGGCCAGCTCCACTACTCAGTCTACGAGGAGGCCAGACACGGCACCTTCGTGGGCCGCAT
  CGCGCAGGACCTGGGGCTGGAGCTGGCGGAGCTGGTGCAGCGCCTGTTCCGGGTGGCGTCCAAAAGACAC
  GGGGACCTTCTGGAGGTAAATCTGCAGAATGGCATTTTGTTTGTGAATTCTCGGATTGACCGCGAGGAGC
  TGTGCGGGCGGAGCGTGGAGTGCAGCATCCACCTGGAGGTGATCGTGGACAGGCCGCTGCAGGTTTTCCA
  TGTGGACGTGGAAGTGAAGGACATTAACGACAACCCGCCCAGGTTCTCCGTAACAGAACAAAAGCTCTCA
  ATACCTGAATCCAGACTGCTTGACTCTCGATTTCCACTAGAAGGCGCATCTGATGCGGATGTTGGAGAGA
  ACGCATTGCTTACTTACAAACTCAGTCCAAATGAGTATTTTGTTCTTGATATTATAAACAAAAAAGACAA
  AGACAAATTCCCAGTGCTTGTTCTGCGGAAGCTGCTGGATCGTGAAGAAAATCCTCAGCTAAAGTTGTTG
  TTGACAGCAACTGATGGAGGCAAACCTGAATTTACCGGATCTGTTTCTCTGCTGATCCTGGTGTTAGATG
  CCAATGATAACGCCCCTATCTTTGACAGACCGGTTTATGAAGTTAAGATGTATGAAAATCAAGTGAACCA
  AACATTAGTAATACGGCTCAACGCTTCTGATTCGGATGAAGGAATAAACAAGGAAATGATGTATTCATTT
  AGCTCTTTGGTCCCACCCACGATAAGAAGGAAATTTTGGATAAACGAAAGGACGGGAGAAATAAAAGTAA
  ATGATGCTATTGACTTTGAGGACAGTAACACTTATGAAATTCATGTAGATGTTACAGATAAGGGAAACCC
  ACCTATGGTTGGTCACTGCACGGTCCTAGTGGAACTACTGGATGAAAATGATAATTCACCTGAGGTGATT
  GTCACTTCTCTGTCTCTCCCAGTGAAAGAAGATGCTCAAGTGGGCACCGTCATTGCCCTAATCAGCGTTT
  CTGACCATGATTCAGGAGCCAACGGACAGGTCACCTGCTCTCTGACGCCTCACGTTCCGTTCAAGCTGGT
  GTCCACCTACAAGAATTACTACTCATTGGTGCTGGACAGCGCTCTGGACCGCGAGAGGGTGTCGGCCTAT
  GAGCTGGTGGTGACCGCGCGGGACGGGGGCTCGCCTCCGCTGTGGGCCACGGCCAGCGTGTCTGTGGAGG
  TGGCCGACGTGAACGACAACGCGCCTGCGTTCGCGCAGTCCGAGTACACGGTGTTCGTGAAGGAGAACAA
  CCCGCCAGGCTGCCACATCTTCACGGTGTCTGCGTGGGACGCGGACGCGCAGGAGAACGCCCTGGTGTCC
  TACTCTCTGGTGGAGCGGCGGTTGGGCGAGCGCTCGCTGTCGAGCTACGTGTCGGTGCACGCGGAGAGCG
  GCAAGGTGTACGCGCTGCAGCCGCTGGACCACGAGGAGCTGGAGCTGCTACAGTTCCAGCCACGACAGCC
  CAACCCTGACTGGCGTTACTCTGCCTCCCTGAGAGCAGGCATGCACAGCTCTGTGCACCTAGAGGAGGCT
  GGCATTCTACGGGCTGGTCCAGGAGGGCCTGATCAGCAGTGGCCAACAGTATCCAGTGCAACACCAGAAC
  CAGAGGCAGGAGAAGTGTCCCCTCCAGTCGGTGCGGGTGTCAACAGCAACAGCTGGACCTTTAAATACGG
  ACCAGGCAACCCCAAACAATCCGGTCCCGGTGAGTTGCCCGACAAATTCATTATCCCAGGATCTCCTGCA
  ATCATCTCCATCCGGCAGGAGCCTACTAACAGCCAAATTGACAAAAGTGACTTCATAACCTTCGGCAAAA
  AGGAGGAGACCAAGAAAAAGAAGAAAAAGAAGAAGGGTAACAAGACCCAGGAGAAAAAAGAGAAAGGGAA
  CAGCACGACTGACAACAGTGACCAG
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG224718 representing NM_031860
  Red=Cloning site Green=Tags(s)
  
  MVSRCSCLGVQCLLLSLLLLAAWEVGSGQLHYSVYEEARHGTFVGRIAQDLGLELAELVQRLFRVASKRH
  GDLLEVNLQNGILFVNSRIDREELCGRSVECSIHLEVIVDRPLQVFHVDVEVKDINDNPPRFSVTEQKLS
  IPESRLLDSRFPLEGASDADVGENALLTYKLSPNEYFVLDIINKKDKDKFPVLVLRKLLDREENPQLKLL
  LTATDGGKPEFTGSVSLLILVLDANDNAPIFDRPVYEVKMYENQVNQTLVIRLNASDSDEGINKEMMYSF
  SSLVPPTIRRKFWINERTGEIKVNDAIDFEDSNTYEIHVDVTDKGNPPMVGHCTVLVELLDENDNSPEVI
  VTSLSLPVKEDAQVGTVIALISVSDHDSGANGQVTCSLTPHVPFKLVSTYKNYYSLVLDSALDRERVSAY
  ELVVTARDGGSPPLWATASVSVEVADVNDNAPAFAQSEYTVFVKENNPPGCHIFTVSAWDADAQENALVS
  YSLVERRLGERSLSSYVSVHAESGKVYALQPLDHEELELLQFQPRQPNPDWRYSASLRAGMHSSVHLEEA
  GILRAGPGGPDQQWPTVSSATPEPEAGEVSPPVGAGVNSNSWTFKYGPGNPKQSGPGELPDKFIIPGSPA
  IISIRQEPTNSQIDKSDFITFGKKEETKKKKKKKKGNKTQEKKEKGNSTTDNSDQ
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_031860
• ORF Size: 2055 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_031860.1, NP_114066.1
• RefSeq Size: 4465
• RefSeq ORF: 2058
• Locus ID: 56139
• Protein Families: Secreted Protein
• Gene Summary: This gene is a member of the protocadherin alpha gene cluster, one of three related gene clusters tandemly linked on chromosome five that demonstrate an unusual genomic organization similar to that of B-cell and T-cell receptor gene clusters. The alpha gene cluster is composed of 15 cadherin superfamily genes related to the mouse CNR genes and consists of 13 highly similar and 2 more distantly related coding sequences. The tandem array of 15 N-terminal exons, or variable exons, are followed by downstream C-terminal exons, or constant exons, which are shared by all genes in the cluster. The large, uninterrupted N-terminal exons each encode six cadherin ectodomains while the C-terminal exons encode the cytoplasmic domain. These neural cadherin-like cell adhesion proteins are integral plasma membrane proteins that most likely play a critical role in the establishment and function of specific cell-cell connections in the brain. Alternative splicing has been observed and additional variants have been suggested but their full-length nature has yet to be determined. [provided by RefSeq, Jul 2008]