MPP5 (NM_022474) Human Tagged ORF Clone

CAT#: RG224752

MPP5 (GFP-tagged) - Human membrane protein, palmitoylated 5 (MAGUK p55 subfamily member 5) (MPP5)

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: MPP5
• Synonyms: PALS1
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG224752 representing NM_022474
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGACAACATCCCATATGAATGGGCATGTTACAGAGGAATCAGACAGCGAAGTAAAAAATGTTGATCTTG
  CATCACCAGAGGAACATCAGAAGCACCGAGAGATGGCTGTTGACTGCCCTGGAGATTTGGGCACCAGGAT
  GATGCCAATACGTCGAAGTGCACAGTTGGAGCGTATTCGGCAACAACAGGAGGACATGAGGCGTAGGAGA
  GAGGAAGAAGGGAAAAAGCAAGAACTTGACCTTAATTCTTCCATGAGACTTAAGAAACTAGCCCAAATTC
  CTCCAAAGACCGGAATAGATAACCCTATGTTTGATACAGAGGAAGGAATTGTCTTAGAAAGTCCTCATTA
  TGCTGTGAAAATATTAGAAATAGAAGACTTGTTTTCTTCACTTAAACATATCCAACATACTTTGGTAGAT
  TCTCAGAGCCAGGAGGATATTTCACTGCTTTTACAACTTGTTCAAAATAAGGATTTCCAGAATGCATTTA
  AGATACACAATGCCATCACAGTACACATGAACAAGGCCAGTCCTCCATTTCCTCTTATCTCCAACGCACA
  AGATCTTGCTCAAGAGGTACAAACTGTTTTGAAGCCAGTTCATCATAAGGAAGGACAAGAACTAACTGCT
  TTGCTGAATACTCCACATATTCAGGCACTTTTACTGGCCCACGATAAGGTTGCTGAGCAGGAAATGCAGC
  TAGAGCCCATTACAGATGAGAGAGTTTATGAAAGTATTGGCCAGTATGGAGGAGAAACTGTAAAAATAGT
  TCGTATAGAAAAGGCTCGTGATATTCCGTTGGGTGCTACAGTTCGTAATGAAATGGACTCTGTCATCATT
  AGCCGGATAGTAAAAGGGGGTGCTGCAGAGAAAAGTGGTCTGTTGCATGAAGGAGATGAAGTTCTAGAGA
  TTAATGGCATTGAAATTCGGGGGAAAGATGTCAATGAGGTTTTTGACTTGTTGTCTGATATGCATGGTAC
  TTTGACTTTTGTCCTGATTCCCAGTCAACAGATCAAGCCGCCTCCTGCCAAGGAAACAGTAATCCATGTA
  AAAGCTCATTTTGACTATGACCCCTCAGATGACCCTTATGTTCCATGTCGAGAGTTAGGTCTGTCTTTTC
  AAAAAGGTGATATACTTCATGTGATCAGTCAAGAAGATCCAAACTGGTGGCAGGCCTACAGGGAAGGGGA
  CGAAGATAATCAACCTCTAGCCGGGCTTGTTCCAGGGAAAAGCTTTCAGCAGCAAAGGGAAGCCATGAAA
  CAAACCATAGAAGAAGATAAGGAGCCAGAAAAATCAGGAAAACTGTGGTGTGCAAAGAAGAATAAAAAGA
  AGAGGAAAAAGGTTTTATATAATGCCAATAAAAATGATGATTATGACAACGAGGAGATCTTAACCTATGA
  GGAAATGTCACTTTATCATCAGCCAGCAAATAGGAAGAGACCTATCATCTTGATTGGTCCACAGAACTGT
  GGCCAGAATGAATTGCGTCAGAGGCTCATGAACAAAGAAAAGGACCGCTTTGCATCTGCAGTTCCTCATA
  CAACCCGGAGTAGGCGAGACCAAGAAGTAGCCGGTAGAGATTACCACTTTGTTTCGCGGCAAGCATTCGA
  GGCAGACATAGCAGCTGGAAAGTTCATTGAGCATGGTGAATTTGAGAAGAATTTGTATGGAACTAGCATA
  GATTCTGTACGGCAAGTGATCAACTCTGGCAAAATATGTCTTTTAAGTCTTCGTACACAGTCATTGAAGA
  CTCTCCGGAATTCAGATTTGAAACCATATATTATCTTCATTGCACCCCCTTCACAAGAAAGACTTCGGGC
  ATTATTGGCCAAAGAAGGCAAGAATCCAAAGCCTGAAGAGTTGAGAGAAATCATTGAGAAGACAAGAGAG
  ATGGAGCAGAACAATGGCCACTACTTTGATACGGCAATTGTGAATTCCGATCTTGATAAAGCCTATCAGG
  AATTGCTTAGGTTAATTAACAAACTTGATACTGAACCTCAGTGGGTACCATCCACTTGGCTGAGG
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG224752 representing NM_022474
  Red=Cloning site Green=Tags(s)
  
  MTTSHMNGHVTEESDSEVKNVDLASPEEHQKHREMAVDCPGDLGTRMMPIRRSAQLERIRQQQEDMRRRR
  EEEGKKQELDLNSSMRLKKLAQIPPKTGIDNPMFDTEEGIVLESPHYAVKILEIEDLFSSLKHIQHTLVD
  SQSQEDISLLLQLVQNKDFQNAFKIHNAITVHMNKASPPFPLISNAQDLAQEVQTVLKPVHHKEGQELTA
  LLNTPHIQALLLAHDKVAEQEMQLEPITDERVYESIGQYGGETVKIVRIEKARDIPLGATVRNEMDSVII
  SRIVKGGAAEKSGLLHEGDEVLEINGIEIRGKDVNEVFDLLSDMHGTLTFVLIPSQQIKPPPAKETVIHV
  KAHFDYDPSDDPYVPCRELGLSFQKGDILHVISQEDPNWWQAYREGDEDNQPLAGLVPGKSFQQQREAMK
  QTIEEDKEPEKSGKLWCAKKNKKKRKKVLYNANKNDDYDNEEILTYEEMSLYHQPANRKRPIILIGPQNC
  GQNELRQRLMNKEKDRFASAVPHTTRSRRDQEVAGRDYHFVSRQAFEADIAAGKFIEHGEFEKNLYGTSI
  DSVRQVINSGKICLLSLRTQSLKTLRNSDLKPYIIFIAPPSQERLRALLAKEGKNPKPEELREIIEKTRE
  MEQNNGHYFDTAIVNSDLDKAYQELLRLINKLDTEPQWVPSTWLR
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_022474
• ORF Size: 2025 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_022474.2, NP_071919.2
• RefSeq Size: 5375
• RefSeq ORF: 2028
• Locus ID: 64398
• Domains: SH3, PDZ, L27, Guanylate_kin, GuKc
• Protein Families: Druggable Genome
• Protein Pathways: Tight junction
• Gene Summary: This gene encodes a member of the p55-like subfamily of the membrane-associated guanylate kinase (MAGUK) gene superfamily. The encoded protein participates in the polarization of differentiating cells, has been shown to regulate myelinating Schwann cells (PMID: 20237282), and is one of the components of the Crumbs complex in the retina. Mice which express lower levels of the orthologous protein have retinal degeneration and impaired vision (PMID: 22114289). Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Feb 2012]