BNIP1 (NM_013978) Human Tagged ORF Clone

CAT#: RG224932

BNIP1 (GFP-tagged) - Human BCL2/adenovirus E1B 19kDa interacting protein 1 (BNIP1), transcript variant BNIP1-a

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: BNIP1
• Synonyms: NIP1; SEC20; TRG-8
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG224932 representing NM_013978
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGGCTCCCCAAGACGTCCACGTCCGGATCTGTAACCAAGAGATTGTCAAATTTGACCTGGAGGTGA
  AGGCGCTTATTCAGGATATCCGTGATTGTTCAGGACCCTTAAGTGCTCTTACTGAACTGAATACTAAAGT
  AAAAGAGAAATTTCAACAGTTGCGTCACAGAATACAGGACCTGGAGCAGTTGGCTAAAGAGCAAGACAAA
  GAATCAGAGAAACAACTTCTACTCCAGGAAGTGGAGAATCACAAAAAGCAGATGCTCAGGAAAACCACCA
  AAGAGAGCCTGGCCCAGACATCCAGTACCATCACTGAGAGCCTCATGGGGATCAGCAGGATGATGGCCCA
  GCAGGTCCAGCAGAGCGAGGAGGCCATGCAGTCTCTAGTCACTTCTTCACGAACGATCCTGGATGCAAAT
  GAAGAATTTAAGTCCATGTCGGGCACCATCCAGCTGGGCCGGAAGCTTATCACAAAATACAATCGCCGGG
  AGCTGACGGACAAGCTTCTCATCTTCCTTGCGCTACGCCTGTTTCTTGCTACGGTCCTCTATATTGTGAA
  AAAGCGGCTCTTTCCATTTTTG
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG224932 representing NM_013978
  Red=Cloning site Green=Tags(s)
  
  MAAPQDVHVRICNQEIVKFDLEVKALIQDIRDCSGPLSALTELNTKVKEKFQQLRHRIQDLEQLAKEQDK
  ESEKQLLLQEVENHKKQMLRKTTKESLAQTSSTITESLMGISRMMAQQVQQSEEAMQSLVTSSRTILDAN
  EEFKSMSGTIQLGRKLITKYNRRELTDKLLIFLALRLFLATVLYIVKKRLFPFL
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_013978
• ORF Size: 582 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_013978.1, NP_053581.1
• RefSeq Size: 722 bp
• RefSeq ORF: 585 bp
• Locus ID: 662
• Cytogenetics: 5q35.1
• Domains: Sec20
• Protein Families: Druggable Genome, Transmembrane
• Protein Pathways: SNARE interactions in vesicular transport
• Gene Summary: 'This gene is a member of the BCL2/adenovirus E1B 19 kd-interacting protein (BNIP) family. It interacts with the E1B 19 kDa protein, which protects cells from virally-induced cell death. The encoded protein also interacts with E1B 19 kDa-like sequences of BCL2, another apoptotic protector. In addition, this protein is involved in vesicle transport into the endoplasmic reticulum. Alternative splicing of this gene results in four protein products with identical N- and C-termini. [provided by RefSeq, Mar 2011]'