BNIP1 (NM_013978) Human Untagged Clone

CAT#: SC108988

BNIP1 (untagged)-Human BCL2/adenovirus E1B 19kDa interacting protein 1 (BNIP1), transcript variant BNIP1-a

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: BNIP1
• Synonyms: NIP1; SEC20; TRG-8
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_013978, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGGCGGCTCCCCAAGACGTCCACGTCCGGATCTGTAACCAAGAGATTGTCAAATTTGACCTGGAGGTGA
  AGGCGCTTATTCAGGATATCCGTGATTGTTCAGGACCCTTAAGTGCTCTTACTGAACTGAATACTAAAGT
  AAAAGAGAAATTTCAACAGTTGCGTCACAGAATACAGGACCTGGAGCAGTTGGCTAAAGAGCAAGACAAA
  GAATCAGAGAAACAACTTCTACTCCAGGAAGTGGAGAATCACAAAAAGCAGATGCTCAGGAAAACCACCA
  AAGAGAGCCTGGCCCAGACATCCAGTACCATCACTGAGAGCCTCATGGGGATCAGCAGGATGATGGCCCA
  GCAGGTCCAGCAGAGCGAGGAGGCCATGCAGTCTCTAGTCACTTCTTCACGAACGATCCTGGATGCAAAT
  GAAGAATTTAAGTCCATGTCGGGCACCATCCAGCTGGGCCGGAAGCTTATCACAAAATACAATCGCCGGG
  AGCTGACGGACAAGCTTCTCATCTTCCTTGCGCTAGCCCTGTTTCTTGCTACGGTCCTCTATATTGTGAA
  AAAGCGGCTCTTTCCATTTTTGTGA
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_013978 unedited
  ATTTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGGGGCTCCCCAAA
  ACGTCCACGTCCGGATCTGTAACCAAGAGATTGTCAAATTTGACCTGGAGGTGAAGGCGC
  TTATTCAGGATATCCGTGATTGTTCAGGACCCTTAAGTGCTCTTACTGAACTGAATACTA
  AAGTAAAAGCGAAATTTCAACAGTTGCGTCACAGAATACAGGACCTGGAGCAGTTGGCTA
  AAGAGCAAGACAAAGAATCAGAGAAACAACTTCTACTCCAGGAAGTGGAGAATCACAAAA
  AGCAGATGCTCAGCAATCAGGCCTCATGGAGGAAAGCTAATCTCACCTGCAAAATTGCAA
  TCGACAATCTAGAGAAAGCAGAACTTCTTCAGGGAGGAGATCTCTTAAGGCAAAGGAAAA
  CCACCAAAGAGAGCCTGGCCCAGACATCCAGTACCATCACTGAGAGCCTCATGGGGATCA
  GCAGGATGATGGCCCAGCAGGTCCAGCAGAGCGAGGAGGCCATGCAGTCTCTAGTCACTT
  CTTCACGAACGATCCTGGATGCAAATGAAGAATTTAAGTCCATGTCGGGCACCATCCAGC
  TGGGCCGGAAGCTTATCACAAAATACAATCGCCGGGAGCTGACGGACAAGCTTCTCATCT
  TCCTTGCGCTAGCCCTGTTTCTTGCTACGGTCCTCTATATTGTGAAAAAGCGGCTCTTTC
  CATTTTTGTGAGATCCCAAAGGTGCCAGNTCTGGCCCTTTCAGCTCCTGNTTCANGATCT
  GTCCTGGTTCCTGAGCTCTNAGCTGCTAAGCTGAGCACACACCCCTNNCGTTGCACCAGT
  TGCCTGCAGGTGGNATGGAAACACAGTGCCCACTTTTCTGCAGTAGCTGGCTTGTAAAGG
  GTGACAAAGCCATGGNAGAANGTCTNNGCATGGATGCCGCCTA
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_013978 unedited
  TGAACCGCGNGCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT
  TTTTTTTTTTTTTTTTTTTTTTCTATTAAAAAAAATCAAGTTTATTCAAGGAATGACCAG
  GAGTTTTCATCACCTTTTTTCCTACCTTTTTTTTTTTCCCTCCACCAAATTTGGCTTTTA
  AGGGCCCAATGGGACCGGGAAACAGGAGGGGCATCCGGCCCTGGAGGCTGGACTTTAAGG
  GCTCCACCCAAAAGCGGCCTCCCATAGTGCACAATGGGGGAAGGAGGCGGTTCTTATGTC
  CCCAGGGCGGCATCCCAATGCCAAACCTTCCTCCCATGGCTTTGTTCACCCTTTACAAGC
  CAGCTACTTGCAAAAAAGGGGGGCACTGTGTTCCATCCAACCTGCAGGCAACTGGGGCAA
  ACGGAGGGGGGGGGGGCTCAGCTTACCACCCTAAAGCTCAGGAACCAGGACAGATCCTGA
  AACAGGAGCTGAAAGGGCCAAAACTGGCACCTTTGGGATCTCACAAAAATGGAAAGAGCC
  GTTTTTTCACAATATAAAGGACCGTACCAAAAAACAGGGCTAGCCCAAGGAAAATAAAAA
  CCTTGTCCGTCACCTCCCGGCGATTGTATTTTGTGATAACCTTCCGGCCCAGCTGGATGG
  TGCCCGACATGGACTTAAATTCTCCATTTGCATCCAGGATCGTTCGTGAAGAAGTGACTA
  GAGACTGCATGGCCTCCTCGCTCTGCTGGACCTGCTGGGCCATCATCCTGCTGATCCCCA
  TGAGGCTCTCANNGATGGTACTGGATGTCTGGGCCCAGCTCTCTTTGNNGTTTTTCCTTT
  GCCTTAAGAGATCTCTCCCTGGAAAAGNTCTGCTTTCTCTAGATGGNCGATCGCAATTNT
  GCAGGTGAAATACCTTTCCTCCATGAGGCCTGATGCTGAGCATTGCTTTTGGGATA
  
• Restriction Sites: NotI-NotI
• ACCN: NM_013978
• Insert Size: 1260 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_013978.1, NP_053581.1
• RefSeq Size: 722 bp
• RefSeq ORF: 585 bp
• Locus ID: 662
• Cytogenetics: 5q35.1
• Domains: Sec20
• Protein Families: Druggable Genome, Transmembrane
• Protein Pathways: SNARE interactions in vesicular transport
• Gene Summary: 'This gene is a member of the BCL2/adenovirus E1B 19 kd-interacting protein (BNIP) family. It interacts with the E1B 19 kDa protein, which protects cells from virally-induced cell death. The encoded protein also interacts with E1B 19 kDa-like sequences of BCL2, another apoptotic protector. In addition, this protein is involved in vesicle transport into the endoplasmic reticulum. Alternative splicing of this gene results in four protein products with identical N- and C-termini. [provided by RefSeq, Mar 2011]'
  Transcript Variant: Transcript variant BNIP1-a contains a 102-nucleotide in-frame deletion as compared to the full-length BNIP1 variant. This variant lacks the BH3 domain which is associated with pro-apoptotic function, and therefore, may function similarly to anti-apoptotic members of the BCL2 family.