PRKAG2 (NM_016203) Human Tagged ORF Clone

CAT#: RG224953

PRKAG2 (GFP-tagged) - Human protein kinase, AMP-activated, gamma 2 non-catalytic subunit (PRKAG2), transcript variant a

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: PRKAG2
• Synonyms: AAKG; AAKG2; CMH6; H91620p; WPWS
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG224953 representing NM_016203
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGAAGCGCGGTTATGGACACCAAGAAGAAAAAAGATGTTTCCAGCCCCGGCGGGAGCGGCGGCAAGA
  AAAATGCCAGCCAGAAGAGGCGTTCGCTGCGCGTGCACATTCCGGACCTGAGCTCCTTCGCCATGCCGCT
  CCTGGACGGAGACCTGGAGGGTTCCGGAAAGCATTCCTCTCGAAAGGTGGACAGCCCCTTCGGCCCGGGC
  AGCCCCTCCAAAGGGTTCTTCTCCAGAGGCCCCCAGCCCCGGCCCTCCAGCCCCATGTCTGCACCTGTGA
  GGCCCAAGACCAGCCCCGGCTCTCCCAAAACCGTGTTCCCGTTCTCCTACCAGGAGTCCCCGCCACGCTC
  CCCTCGACGCATGAGCTTCAGTGGGATCTTCCGCTCCTCCTCCAAAGAGTCTTCCCCCAACTCCAACCCT
  GCTACCTCGCCCGGGGGCATCAGGTTTTTCTCCCGCTCCAGAAAAACCTCCGGCCTCTCCTCCTCTCCGT
  CAACACCCACCCAAGTGACCAAGCAGCACACGTTTCCCCTGGAATCCTATAAGCACGAGCCTGAACGGTT
  AGAGAATCGCATCTATGCCTCGTCTTCCCCCCCGGACACAGGGCAGAGGTTCTGCCCGTCTTCCTTCCAG
  AGCCCGACCAGGCCTCCACTGGCATCACCGACACACTATGCTCCCTCCAAAGCCGCGGCGCTGGCGGCGG
  CCCTGGGACCCGCGGAAGCCGGCATGCTGGAGAAGCTGGAGTTCGAGGACGAAGTAGAAGACTCAGAAAG
  TGGTGTTTACATGCGATTCATGAGGTCACACAAGTGTTATGACATCGTTCCAACCAGTTCAAAGCTTGTT
  GTCTTTGATACTACATTACAAGTTAAAAAGGCCTTCTTTGCTTTGGTAGCCAACGGTGTCCGAGCAGCGC
  CACTGTGGGAGAGTAAAAAACAAAGTTTTGTAGGAATGCTAACAATTACAGATTTCATAAATATACTACA
  TAGATACTATAAATCACCTATGGTACAGATTTATGAATTAGAGGAACATAAAATTGAAACATGGAGGGAG
  CTTTATTTACAAGAAACATTTAAGCCTTTAGTGAATATATCTCCAGATGCAAGCCTCTTCGATGCTGTAT
  ACTCCTTGATCAAAAATAAAATCCACAGATTGCCCGTTATTGACCCTATCAGTGGGAATGCACTTTATAT
  ACTTACCCACAAAAGAATCCTCAAGTTCCTCCAGCTTTTTATGTCTGATATGCCAAAGCCTGCCTTCATG
  AAGCAGAACCTGGATGAGCTTGGAATAGGAACGTACCACAACATTGCCTTCATACATCCAGACACTCCCA
  TCATCAAAGCCTTGAACATATTTGTGGAAAGACGAATATCAGCTCTGCCTGTTGTGGATGAGTCAGGAAA
  AGTTGTAGATATTTATTCCAAATTTGATGTAATTAATCTTGCTGCTGAGAAAACATACAATAACCTAGAT
  ATCACGGTGACCCAGGCCCTTCAGCACCGTTCACAGTATTTTGAAGGTGTTGTGAAGTGCAATAAGCTGG
  AAATACTGGAGACCATCGTGGACAGAATAGTAAGAGCTGAGGTCCATCGGCTGGTGGTGGTAAATGAAGC
  AGATAGTATTGTGGGTATTATTTCCCTGTCGGACATTCTGCAAGCCCTGATCCTCACACCAGCAGGTGCC
  AAACAAAAGGAGACAGAAACGGAG
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG224953 representing NM_016203
  Red=Cloning site Green=Tags(s)
  
  MGSAVMDTKKKKDVSSPGGSGGKKNASQKRRSLRVHIPDLSSFAMPLLDGDLEGSGKHSSRKVDSPFGPG
  SPSKGFFSRGPQPRPSSPMSAPVRPKTSPGSPKTVFPFSYQESPPRSPRRMSFSGIFRSSSKESSPNSNP
  ATSPGGIRFFSRSRKTSGLSSSPSTPTQVTKQHTFPLESYKHEPERLENRIYASSSPPDTGQRFCPSSFQ
  SPTRPPLASPTHYAPSKAAALAAALGPAEAGMLEKLEFEDEVEDSESGVYMRFMRSHKCYDIVPTSSKLV
  VFDTTLQVKKAFFALVANGVRAAPLWESKKQSFVGMLTITDFINILHRYYKSPMVQIYELEEHKIETWRE
  LYLQETFKPLVNISPDASLFDAVYSLIKNKIHRLPVIDPISGNALYILTHKRILKFLQLFMSDMPKPAFM
  KQNLDELGIGTYHNIAFIHPDTPIIKALNIFVERRISALPVVDESGKVVDIYSKFDVINLAAEKTYNNLD
  ITVTQALQHRSQYFEGVVKCNKLEILETIVDRIVRAEVHRLVVVNEADSIVGIISLSDILQALILTPAGA
  KQKETETE
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_016203
• ORF Size: 1704 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_016203.2, NP_057287.2
• RefSeq Size: 2062
• RefSeq ORF: 1710
• Locus ID: 51422
• Domains: CBS
• Protein Families: Druggable Genome
• Protein Pathways: Adipocytokine signaling pathway, Hypertrophic cardiomyopathy (HCM), Insulin signaling pathway
• Gene Summary: AMP-activated protein kinase (AMPK) is a heterotrimeric protein composed of a catalytic alpha subunit, a noncatalytic beta subunit, and a noncatalytic regulatory gamma subunit. Various forms of each of these subunits exist, encoded by different genes. AMPK is an important energy-sensing enzyme that monitors cellular energy status and functions by inactivating key enzymes involved in regulating de novo biosynthesis of fatty acid and cholesterol. This gene is a member of the AMPK gamma subunit family. Mutations in this gene have been associated with Wolff-Parkinson-White syndrome, familial hypertrophic cardiomyopathy, and glycogen storage disease of the heart. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq, Jan 2015]