DDAH1 (NM_001134445) Human Tagged ORF Clone

CAT#: RG225195

DDAH1 (GFP-tagged) - Human dimethylarginine dimethylaminohydrolase 1 (DDAH1), transcript variant 2

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: DDAH1
• Synonyms: DDAH; DDAH-1; DDAHI; HEL-S-16
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG225195 representing NM_001134445
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGATGAAAGAAGCATTAGAAAAACTTCAGCTCAATATAGTAGAGATGAAAGATGAAAATGCAACTTTAG
  ATGGCGGAGATGTTTTATTCACAGGCAGAGAATTTTTTGTGGGCCTTTCCAAAAGGACAAATCAACGAGG
  TGCTGAAATCTTGGCTGATACTTTTAAGGACTATGCAGTCTCCACAGTGCCAGTGGCAGATGGGTTGCAT
  TTGAAGAGTTTCTGCAGCATGGCTGGGCCTAACCTGATCGCAATTGGGTCTAGTGAATCTGCACAGAAGG
  CCCTTAAGATCATGCAACAGATGAGTGACCACCGCTACGACAAACTCACTGTGCCTGATGACATAGCAGC
  AAACTGTATATATCTAAATATCCCCAACAAAGGGCACGTCTTGCTGCACCGAACCCCGGAAGAGTATCCA
  GAAAGTGCAAAGGTTTATGAGAAACTGAAGGACCATATGCTGATCCCCGTGAGCATGTCTGAACTGGAAA
  AGGTGGATGGGCTGCTCACCTGCTGCTCAGTTTTAATTAACAAGAAAGTAGACTCC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG225195 representing NM_001134445
  Red=Cloning site Green=Tags(s)
  
  MMKEALEKLQLNIVEMKDENATLDGGDVLFTGREFFVGLSKRTNQRGAEILADTFKDYAVSTVPVADGLH
  LKSFCSMAGPNLIAIGSSESAQKALKIMQQMSDHRYDKLTVPDDIAANCIYLNIPNKGHVLLHRTPEEYP
  ESAKVYEKLKDHMLIPVSMSELEKVDGLLTCCSVLINKKVDS
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001134445
• ORF Size: 546 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_001134445.1, NP_001127917.1
• RefSeq Size: 4023
• RefSeq ORF: 549
• Locus ID: 23576
• Gene Summary: This gene belongs to the dimethylarginine dimethylaminohydrolase (DDAH) gene family. The encoded enzyme plays a role in nitric oxide generation by regulating cellular concentrations of methylarginines, which in turn inhibit nitric oxide synthase activity. [provided by RefSeq, Jul 2008]