USP46 (NM_001134223) Human Tagged ORF Clone

CAT#: RG225534

USP46 (GFP-tagged) - Human ubiquitin specific peptidase 46 (USP46), transcript variant 2

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: USP46
• Synonyms: FLJ11850; FLJ12552; FLJ14283; FLJ39393
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG225534 representing NM_001134223
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAATTGTTTTCAGGGCACCAATGCCTCTGCTCTGGAAAAAGACATTGGTCCAGAGCAGTTTCCAATCA
  ATGAACACTATTTCGGATTGGTCAATTTTGGAAACACATGCTACTGTAACTCCGTGCTTCAGGCATTGTA
  CTTCTGCCGTCCATTCCGGGAGAATGTGTTGGCATACAAGGCCCAGCAAAAGAAGAAGGAAAACTTGCTG
  ACGTGCCTGGCGGACCTTTTCCACAGCATTGCCACACAGAAGAAGAAGGTTGGCGTCATCCCACCAAAGA
  AGTTCATTTCAAGGCTGAGAAAAGAGAATGATCTCTTTGATAACTACATGCAGCAGGATGCTCATGAATT
  TTTAAATTATTTGCTAAACACTATTGCGGACATCCTTCAGGAGGAGAAGAAACAGGAAAAACAAAATGGA
  AAATTAAAAAATGGCAACATGAACGAACCTGCGGAAAATAATAAACCAGAACTCACCTGGGTCCATGAGA
  TTTTTCAGGGAACGCTTACCAATGAAACTCGATGCTTGAACTGTGAAACTGTTAGTAGCAAAGATGAAGA
  TTTTCTTGACCTTTCTGTTGATGTGGAGCAGAATACATCCATTACCCACTGTCTAAGAGACTTCAGCAAC
  ACAGAAACACTGTGTAGTGAACAAAAATATTATTGTGAAACATGCTGCAGCAAACAAGAAGCCCAGAAAA
  GGATGAGGGTAAAAAAGCTGCCCATGATCTTGGCCCTGCACCTAAAGCGGTTCAAGTACATGGAGCAGCT
  GCACAGATACACCAAGCTGTCTTACCGTGTGGTCTTCCCTCTGGAACTCCGGCTCTTCAACACCTCCAGT
  GATGCAGTGAACCTGGACCGCATGTATGACTTGGTTGCGGTGGTCGTTCACTGTGGCAGTGGTCCTAATC
  GTGGGCATTATATCACTATTGTGAAAAGTCACGGCTTCTGGCTTTTGTTTGATGATGACATTGTAGAGAA
  AATAGATGCTCAAGCTATTGAAGAATTCTATGGCCTGACGTCAGATATATCAAAAAATTCAGAATCTGGA
  TATATTTTATTCTATCAGTCAAGAGAG
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG225534 representing NM_001134223
  Red=Cloning site Green=Tags(s)
  
  MNCFQGTNASALEKDIGPEQFPINEHYFGLVNFGNTCYCNSVLQALYFCRPFRENVLAYKAQQKKKENLL
  TCLADLFHSIATQKKKVGVIPPKKFISRLRKENDLFDNYMQQDAHEFLNYLLNTIADILQEEKKQEKQNG
  KLKNGNMNEPAENNKPELTWVHEIFQGTLTNETRCLNCETVSSKDEDFLDLSVDVEQNTSITHCLRDFSN
  TETLCSEQKYYCETCCSKQEAQKRMRVKKLPMILALHLKRFKYMEQLHRYTKLSYRVVFPLELRLFNTSS
  DAVNLDRMYDLVAVVVHCGSGPNRGHYITIVKSHGFWLLFDDDIVEKIDAQAIEEFYGLTSDISKNSESG
  YILFYQSRE
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001134223
• ORF Size: 1077 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_001134223.1, NP_001127695.1
• RefSeq Size: 8057
• RefSeq ORF: 1080
• Locus ID: 64854
• Protein Families: Druggable Genome, Protease
• Gene Summary: Modification of cellular proteins by ubiquitin is an essential regulatory mechanism controlled by the coordinated action of multiple ubiquitin-conjugating and deubiquitinating enzymes. USP46 belongs to a large family of cysteine proteases that function as deubiquitinating enzymes (Quesada et al., 2004 [PubMed 14715245]). [supplied by OMIM, Jun 2009]