Neurofilament (NEFM) (NM_001105541) Human Tagged ORF Clone
CAT#: RG225929
- TrueORF®
NEFM (GFP-tagged) - Human neurofilament, medium polypeptide (NEFM), transcript variant 2. Note: ORF is codon optimized
"NM_001105541" in other vectors (2)
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Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | TurboGFP |
Symbol | NEFM |
Synonyms | NEF3; NF-M; NFM |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RG225929 ORF sequence, codon optimized.
Due to the complexity of NM_001105541, the ORF clone is codon optimized for mammalian Expression. The nucleotide sequence differs from the reference sequence, yet the amino acid sequence remains identical. Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCCAGACATCTTAGAGAGTACCAGGATCTCCTTAACGTCAAGATGGCGCTGGATATAGAAATTGCCG CCTATCGCAAGCTTCTGGAGGGCGAGGAAACAAGATTTTCCACCTTTGCCGGGTCAATCACCGGCCCCCT GTACACTCACAGACCACCCATTACCATCTCTTCCAAAATTCAGAAGCCAAAGGTCGAGGCTCCAAAATTG AAGGTGCAACACAAGTTTGTTGAAGAGATCATTGAGGAAACAAAAGTGGAAGACGAGAAGAGCGAGATGG AGGAGGCCCTCACGGCCATCACCGAAGAGCTCGCTGTCAGCATGAAGGAAGAGAAAAAGGAAGCCGCCGA AGAGAAAGAGGAGGAACCAGAAGCTGAGGAAGAGGAGGTGGCTGCCAAGAAGAGTCCAGTCAAGGCCACT GCCCCTGAGGTGAAAGAGGAGGAGGGGGAGAAAGAGGAAGAGGAAGGCCAGGAAGAGGAGGAGGAAGAAG ATGAAGGAGCCAAGAGCGACCAGGCCGAAGAAGGAGGGAGCGAAAAAGAAGGCAGCTCCGAAAAAGAGGA GGGGGAGCAGGAGGAAGGCGAAACTGAGGCGGAGGCCGAAGGCGAAGAGGCCGAAGCTAAAGAGGAGAAA AAGGTCGAAGAGAAGAGCGAAGAGGTCGCCACGAAGGAGGAGCTGGTAGCCGATGCTAAGGTTGAGAAAC CTGAAAAAGCTAAATCTCCCGTGCCCAAGTCACCGGTGGAGGAGAAGGGAAAATCTCCCGTACCCAAAAG CCCGGTTGAAGAAAAAGGCAAAAGTCCCGTCCCTAAGAGTCCCGTGGAGGAGAAAGGTAAATCACCAGTG CCTAAGTCACCAGTGGAAGAGAAAGGCAAGAGTCCTGTGAGTAAGAGTCCTGTTGAGGAAAAGGCTAAGT CCCCGGTCCCCAAGTCCCCGGTTGAGGAGGCCAAGTCAAAGGCCGAGGTCGGAAAAGGAGAACAAAAAGA GGAGGAGGAGAAGGAAGTCAAAGAAGCACCTAAGGAAGAAAAAGTGGAGAAAAAGGAGGAGAAGCCTAAA GATGTGCCCGAGAAGAAGAAGGCTGAAAGTCCAGTGAAAGAGGAGGCAGTAGCGGAGGTTGTCACGATCA CAAAGAGCGTGAAGGTGCATCTGGAAAAGGAGACAAAGGAGGAAGGCAAGCCACTTCAACAGGAGAAAGA GAAGGAGAAGGCAGGCGGTGAGGGCGGAAGTGAAGAAGAAGGGTCAGATAAGGGCGCTAAAGGTTCTCGA AAGGAGGATATCGCCGTTAACGGAGAGGTAGAAGGTAAAGAGGAGGTCGAGCAGGAGACTAAGGAAAAAG GCTCTGGGAGGGAGGAAGAGAAAGGAGTGGTCACGAACGGACTGGATCTCAGCCCTGCCGACGAGAAGAA GGGCGGAGATAAAAGTGAAGAGAAGGTGGTAGTCACTAAGACTGTAGAAAAAATTACCTCTGAAGGAGGG GACGGCGCCACAAAATACATCACAAAGTCTGTAACCGTGACTCAAAAAGTGGAGGAACATGAAGAGACTT TTGAAGAGAAACTGGTGTCTACCAAGAAAGTCGAGAAAGTGACCTCCCATGCTATAGTGAAGGAGGTGAC TCAATCAGAC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG225929 representing NM_001105541
Red=Cloning site Green=Tags(s) MARHLREYQDLLNVKMALDIEIAAYRKLLEGEETRFSTFAGSITGPLYTHRPPITISSKIQKPKVEAPKL KVQHKFVEEIIEETKVEDEKSEMEEALTAITEELAVSMKEEKKEAAEEKEEEPEAEEEEVAAKKSPVKAT APEVKEEEGEKEEEEGQEEEEEEDEGAKSDQAEEGGSEKEGSSEKEEGEQEEGETEAEAEGEEAEAKEEK KVEEKSEEVATKEELVADAKVEKPEKAKSPVPKSPVEEKGKSPVPKSPVEEKGKSPVPKSPVEEKGKSPV PKSPVEEKGKSPVSKSPVEEKAKSPVPKSPVEEAKSKAEVGKGEQKEEEEKEVKEAPKEEKVEKKEEKPK DVPEKKKAESPVKEEAVAEVVTITKSVKVHLEKETKEEGKPLQQEKEKEKAGGEGGSEEEGSDKGAKGSR KEDIAVNGEVEGKEEVEQETKEKGSGREEEKGVVTNGLDLSPADEKKGGDKSEEKVVVTKTVEKITSEGG DGATKYITKSVTVTQKVEEHEETFEEKLVSTKKVEKVTSHAIVKEVTQSD TRTRPLE - GFP Tag - V |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_001105541 |
ORF Size | 1620 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers. |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_001105541.1, NP_001099011.1 |
RefSeq Size | 2514 bp |
RefSeq ORF | 1623 bp |
Locus ID | 4741 |
Cytogenetics | 8p21.2 |
Protein Pathways | Amyotrophic lateral sclerosis (ALS) |
Gene Summary | 'Neurofilaments are type IV intermediate filament heteropolymers composed of light, medium, and heavy chains. Neurofilaments comprise the axoskeleton and functionally maintain neuronal caliber. They may also play a role in intracellular transport to axons and dendrites. This gene encodes the medium neurofilament protein. This protein is commonly used as a biomarker of neuronal damage. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Oct 2008]' |
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