DIS3 (NM_001128226) Human Tagged ORF Clone

CAT#: RG226264

DIS3 (GFP-tagged) - Human DIS3 mitotic control homolog (S. cerevisiae) (DIS3), transcript variant 2

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: DIS3
• Synonyms: 2810028N01Rik; dis3p; EXOSC11; KIAA1008; RRP44
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG226264 representing NM_001128226
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCTCAAGTCCAAGACGTTCTTAAAAAAGACCCGGGCGGGCGGCGTGATGAAGATCGTGCGCGAGCACT
  ACCTGCGAGACGACATCGGCTGCGGTGCGCCCGGGTGCGCAGCGTGTGGAGGGGCGCACGAGGGGCCGGC
  CCTGGAGCCGCAGCCCCAGGACCCGGCGAGCAGCGTCTGCCCGCAACCGCACTACTTGCTGCCCGACACT
  AATGTGTTACTGCACCAGATTGTAAGTGCCTGGAGGCCGGGGACCTGGGCTTCTGTGGCCTCCAGCCTGC
  GACTCCCAGGCAGCTTAGAAACCTATGTAGAACAAGAACAGGGAGAAAATGCTAATGACAGGAATGATAG
  AGCGATTCGAGTAGCAGCAAAATGGTACAATGAACATTTGAAAAAAATGTCAGCAGACAACCAGCTGCAA
  GTTATCTTCATAACAAATGACAGGAGAAACAAAGAGAAAGCCATAGAAGAAGGAATACCAGCTTTCACTT
  GTGAAGAATATGTAAAGAGCCTAACTGCTAACCCCGAACTCATAGATCGTCTTGCTTGTTTGTCTGAAGA
  AGGGAATGAAATAGAAAGTGGAAAAATAATATTTTCAGAGCATCTTCCCTTAAGTAAGCTACAGCAAGGC
  ATAAAATCTGGTACATACCTTCAAGGAACATTTAGAGCTAGCAGGGAAAATTACTTGGAAGCTACAGTAT
  GGATTCATGGCGACAATGAAGAAAATAAAGAGATAATCTTACAGGGACTTAAACATTTAAACAGAGCTGT
  TCACGAAGATATTGTGGCTGTGGAGCTTCTCCCCAAGAGTCAGTGGGTAGCACCATCTTCTGTGGTTTTA
  CATGATGAAGGTCAAAATGAAGAAGATGTGGAGAAAGAAGAAGAGACAGAACGAATGCTTAAGACTGCTG
  TAAGCGAGAAAATGTTGAAGCCTACAGGTAGAGTTGTAGGAATAATAAAAAGGAATTGGAGACCATATTG
  TGGCATGCTTTCCAAGTCTGACATTAAGGAGTCAAGAAGACATCTCTTTACACCTGCTGATAAGAGAATC
  CCTCGAATTCGCATAGAAACCAGACAGGCTTCCACATTAGAAGGACGGAGAATTATTGTTGCTATTGATG
  GTTGGCCCAGAAATTCCAGATATCCAAATGGACACTTTGTGAGAAATTTAGGTGATGTTGGAGAGAAAGA
  GACTGAAACAGAAGTTTTGTTACTTGAACACGATGTTCCCCATCAGCCTTTTTCACAGGCTGTTCTTAGT
  TTTCTGCCAAAGATGCCCTGGAGCATTACTGAAAAGGACATGAAAAACCGAGAAGACCTGAGGCATCTGT
  GTATTTGTAGTGTAGACCCACCAGGATGTACTGATATAGACGATGCTCTACATTGTCGAGAACTCGAAAA
  TGGAAATTTGGAGGTTGGTGTTCATATTGCTGATGTGAGCCATTTTATTAGGCCAGGAAATGCCTTGGAT
  CAAGAATCAGCCAGAAGAGGAACAACTGTGTATCTTTGTGAAAAGAGGATTGACATGGTTCCAGAGTTGC
  TTAGCTCTAACTTGTGTTCCTTAAAATGTGACGTGGACAGGCTGGCATTTTCATGTATTTGGGAAATGAA
  TCACAATGCTGAAATCTTAAAAACGAAGTTTACCAAAAGTGTTATTAATTCAAAGGCATCTCTGACGTAT
  GCTGAAGCTCAGTTGAGAATTGATTCAGCAAACATGAATGATGATATTACCACTAGTCTCCGTGGACTGA
  ATAAACTAGCCAAAATTCTGAAGAAAAGAAGGATTGAAAAAGGGGCTTTGACTCTATCCTCTCCTGAAGT
  TCGATTCCACATGGACAGTGAAACTCACGATCCTATAGATCTGCAGACCAAGGAACTTAGGGAAACAAAT
  TCCATGGTTGAAGAATTTATGTTACTTGCCAATATTTCTGTTGCAAAAAAAATTCATGAGGAATTTTCTG
  AACATGCTCTGCTTCGAAAACATCCTGCTCCACCTCCATCAAATTATGAAATTCTTGTTAAGGCAGCCAG
  GTCAAGGAATTTGGAAATTAAGACTGATACAGCCAAGTCTTTGGCTGAGTCTTTGGATCAGGCCGAATCT
  CCTACTTTTCCATATCTAAACACTCTGTTGAGAATATTAGCCACTCGCTGTATGATGCAAGCTGTGTACT
  TCTGTTCTGGAATGGATAATGATTTTCATCACTATGGCTTAGCGTCTCCAATATACACACATTTTACTTC
  ACCCATTAGAAGATACGCAGATGTCATTGTTCATCGGCTTTTGGCTGTGGCTATTGGGGCTGACTGTACT
  TATCCAGAGTTGACAGACAAACACAAGCTTGCAGATATATGTAAAAATCTAAATTTCCGGCACAAAATGG
  CTCAATATGCCCAACGTGCATCAGTGGCTTTTCATACCCAGTTATTCTTCAAAAGCAAAGGAATAGTAAG
  TGAAGAAGCCTATATTTTATTTGTAAGAAAGAATGCCATTGTGGTATTAATTCCAAAGTATGGTTTAGAA
  GGGACAGTCTTTTTTGAAGAAAAGGACAAACCAAACCCACAGCTTATTTATGATGATGAGATACCCTCAC
  TTAAAATAGAAGATACAGTGTTCCATGTATTTGATAAAGTTAAAGTGAAAATCATGTTAGACTCATCTAA
  TCTTCAACATCAGAAGATCCGAATGTCCCTGGTAGAACCACAGATACCAGGAATAAGCATTCCTACTGAT
  ACTTCAAACATGGACCTTAATGGACCAAAGAAAAAGAAGATGAAGCTTGGAAAA
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG226264 representing NM_001128226
  Red=Cloning site Green=Tags(s)
  
  MLKSKTFLKKTRAGGVMKIVREHYLRDDIGCGAPGCAACGGAHEGPALEPQPQDPASSVCPQPHYLLPDT
  NVLLHQIVSAWRPGTWASVASSLRLPGSLETYVEQEQGENANDRNDRAIRVAAKWYNEHLKKMSADNQLQ
  VIFITNDRRNKEKAIEEGIPAFTCEEYVKSLTANPELIDRLACLSEEGNEIESGKIIFSEHLPLSKLQQG
  IKSGTYLQGTFRASRENYLEATVWIHGDNEENKEIILQGLKHLNRAVHEDIVAVELLPKSQWVAPSSVVL
  HDEGQNEEDVEKEEETERMLKTAVSEKMLKPTGRVVGIIKRNWRPYCGMLSKSDIKESRRHLFTPADKRI
  PRIRIETRQASTLEGRRIIVAIDGWPRNSRYPNGHFVRNLGDVGEKETETEVLLLEHDVPHQPFSQAVLS
  FLPKMPWSITEKDMKNREDLRHLCICSVDPPGCTDIDDALHCRELENGNLEVGVHIADVSHFIRPGNALD
  QESARRGTTVYLCEKRIDMVPELLSSNLCSLKCDVDRLAFSCIWEMNHNAEILKTKFTKSVINSKASLTY
  AEAQLRIDSANMNDDITTSLRGLNKLAKILKKRRIEKGALTLSSPEVRFHMDSETHDPIDLQTKELRETN
  SMVEEFMLLANISVAKKIHEEFSEHALLRKHPAPPPSNYEILVKAARSRNLEIKTDTAKSLAESLDQAES
  PTFPYLNTLLRILATRCMMQAVYFCSGMDNDFHHYGLASPIYTHFTSPIRRYADVIVHRLLAVAIGADCT
  YPELTDKHKLADICKNLNFRHKMAQYAQRASVAFHTQLFFKSKGIVSEEAYILFVRKNAIVVLIPKYGLE
  GTVFFEEKDKPNPQLIYDDEIPSLKIEDTVFHVFDKVKVKIMLDSSNLQHQKIRMSLVEPQIPGISIPTD
  TSNMDLNGPKKKKMKLGK
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001128226
• ORF Size: 2784 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_001128226.1, NP_001121698.1
• RefSeq Size: 7577
• RefSeq ORF: 2787
• Locus ID: 22894
• Protein Pathways: RNA degradation