PAG3 (ASAP2) (NM_001135191) Human Tagged ORF Clone

CAT#: RG226279

ASAP2 (GFP-tagged) - Human ArfGAP with SH3 domain, ankyrin repeat and PH domain 2 (ASAP2), transcript variant 2

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: ASAP2
• Synonyms: AMAP2; CENTB3; DDEF2; PAG3; PAP; Pap-alpha; SHAG1
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG226279 representing NM_001135191
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCCGGACCAGATCTCCGTGTCGGAATTCGTGGCCGAGACCCATGAGGACTACAAGGCGCCCACGGCCT
  CCAGCTTCACCACCCGCACGGCGCAGTGCCGGAACACTGTGGCGGCCATCGAGGAGGCTTTGGACGTGGA
  CCGGATGGTTCTTTACAAAATGAAGAAATCCGTGAAAGCAATCAACAGCTCTGGGCTGGCTCACGTGGAA
  AATGAAGAGCAGTACACCCAGGCTCTGGAGAAGTTTGGCGGCAACTGTGTATGCAGAGATGACCCAGATT
  TAGGAAGTGCGTTCCTGAAGTTCTCAGTGTTTACAAAGGAGTTGACAGCACTTTTCAAAAACCTGATTCA
  GAATATGAACAACATAATCTCCTTCCCTTTGGACAGTTTGCTGAAGGGGGACCTGAAAGGAGTGAAAGGG
  GATCTGAAAAAGCCTTTTGATAAAGCTTGGAAGGACTATGAAACAAAAATAACCAAGATAGAAAAGGAGA
  AAAAGGAACACGCCAAGCTCCATGGGATGATTCGGACTGAAATAAGCGGAGCGGAAATTGCCGAAGAGAT
  GGAAAAGGAGAGGCGCTTCTTCCAGCTACAGATGTGCGAGTATCTGCTGAAGGTCAACGAAATCAAGATT
  AAAAAGGGAGTAGATTTACTTCAGAATCTGATCAAATACTTTCATGCCCAATGCAATTTTTTTCAGGATG
  GACTCAAAGCCGTGGAAAGCCTCAAACCTTCCATTGAAACGCTGTCTACGGATCTTCACACGATCAAACA
  GGCCCAGGATGAAGAAAGAAGGCAGTTGATACAGCTTCGAGATATTTTGAAATCCGCATTGCAGGTTGAA
  CAGAAAGAGGACTCCCAAATTCGTCAGAGCACAGCTTATAGCTTACATCAGCCTCAGGGAAACAAGGAAC
  ATGGGACCGAGCGGAACGGCAGCCTCTACAAGAAGAGTGACGGGATCCGAAAAGTGTGGCAGAAAAGGAA
  ATGTTCAGTTAAAAATGGTTTTCTGACCATATCCCATGGTACCGCTAACCGGCCTCCTGCAAAGCTCAAC
  CTGCTAACCTGCCAGGTGAAGACCAACCCTGAGGAGAAGAAGTGCTTTGACCTCATTTCACATGACAGAA
  CTTACCACTTTCAAGCTGAAGATGAACAGGAATGTCAAATATGGATGTCTGTGCTGCAAAATAGCAAAGA
  AGAAGCTTTAAACAATGCATTTAAGGGGGATGACAATACTGGAGAAAATAACATCGTCCAAGAACTGACA
  AAGGAGATCATCTCAGAAGTGCAGAGGATGACGGGCAATGACGTCTGCTGTGACTGTGGGGCGCCAGATC
  CTACATGGCTTTCCACCAACCTGGGCATCCTGACCTGCATCGAGTGTTCCGGAATCCACCGAGAGCTGGG
  GGTTCATTATTCCAGGATGCAGTCCCTGACCTTAGATGTACTGGGAACATCTGAGCTGCTGCTCGCCAAG
  AATATTGGGAATGCAGGCTTTAATGAGATCATGGAATGTTGCCTACCAGCTGAGGACTCAGTCAAACCCA
  ACCCAGGCAGCGACATGAATGCAAGAAAGGACTACATCACAGCCAAGTACATCGAGAGGAGATACGCAAG
  GAAGAAGCACGCGGATAACGCGGCGAAGCTTCACAGTCTTTGCGAGGCCGTCAAAACGAGAGATATTTTT
  GGATTGCTCCAAGCTTATGCTGATGGTGTGGATCTTACGGAAAAAATCCCACTGGCCAACGGACATGAGC
  CGGATGAAACGGCCCTCCACCTTGCAGTCAGATCCGTGGATCGAACCTCTCTTCACATTGTAGACTTTTT
  AGTTCAGAACAGTGGGAACCTGGATAAACAGACAGGGAAAGGCAGCACAGCCCTGCACTACTGCTGCCTG
  ACCGACAATGCCGAGTGCCTCAAGTTGCTCCTGCGGGGGAAGGCCTCCATCGAGATAGCAAACGAGTCAG
  GAGAGACTCCGCTGGACATTGCCAAGCGCCTCAAGCACGAGCACTGTGAGGAGCTGCTGACCCAAGCCTT
  ATCTGGAAGATTTAATTCTCACGTTCACGTTGAATATGAATGGCGACTACTCCACGAAGACCTGGATGAA
  AGTGATGACGACATGGATGAGAAATTGCAGCCCAGTCCCAACCGGCGGGAAGACCGGCCCATCAGCTTCT
  ACCAGCTGGGCTCCAACCAGCTTCAGTCTAACGCTGTATCTTTGGCCAGAGATGCTGCAAACCTTGCCAA
  GGAGAAGCAGAGGGCTTTCATGCCCAGCATCTTGCAGAATGAGACTTACGGAGCCCTCCTGAGTGGCAGC
  CCACCTCCCGCCCAGCCTGCAGCCCCCAGCACCACCAGCGCCCCCCCGCTTCCTCCACGGAATGTTGGCA
  AAGATCCCCTGACCCCCACGCCGCCCCCACCCGTTGCCAAGACGCCCAGCGTAATGGAAGCCTTGAGCCA
  GCCGAGCAAGCCTGCCCCGCCTGGGATCTCACAGATCAGGCCCCCACCTCTGCCCCCACAGCCGCCCAGC
  CGCCTCCCGCAGAAGAAGCCTGCGCCGGGGGCTGACAAGTCCACCCCACTGACCAACAAAGGCCAACCGA
  GAGGACCTGTGGATCTCTCTGCAACGGAAGCTCTGGGTCCTCTGTCCAATGCTATGGTCCTGCAGCCCCC
  TGCACCCATGCCTAGGAAGTCGCAGGCAACCAAGTTGAAGCCTAAGCGGGTGAAAGCGCTCTATAACTGT
  GTGGCTGACAACCCCGATGAGCTCACCTTCTCCGAGGGGGATGTGATCATCGTGGACGGGGAGGAGGACC
  AGGAGTGGTGGATTGGCCACATTGATGGAGATCCTGGTCGCAAAGGCGCATTCCCGGTGTCATTTGTGCA
  CTTTATCGCTGAC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG226279 representing NM_001135191
  Red=Cloning site Green=Tags(s)
  
  MPDQISVSEFVAETHEDYKAPTASSFTTRTAQCRNTVAAIEEALDVDRMVLYKMKKSVKAINSSGLAHVE
  NEEQYTQALEKFGGNCVCRDDPDLGSAFLKFSVFTKELTALFKNLIQNMNNIISFPLDSLLKGDLKGVKG
  DLKKPFDKAWKDYETKITKIEKEKKEHAKLHGMIRTEISGAEIAEEMEKERRFFQLQMCEYLLKVNEIKI
  KKGVDLLQNLIKYFHAQCNFFQDGLKAVESLKPSIETLSTDLHTIKQAQDEERRQLIQLRDILKSALQVE
  QKEDSQIRQSTAYSLHQPQGNKEHGTERNGSLYKKSDGIRKVWQKRKCSVKNGFLTISHGTANRPPAKLN
  LLTCQVKTNPEEKKCFDLISHDRTYHFQAEDEQECQIWMSVLQNSKEEALNNAFKGDDNTGENNIVQELT
  KEIISEVQRMTGNDVCCDCGAPDPTWLSTNLGILTCIECSGIHRELGVHYSRMQSLTLDVLGTSELLLAK
  NIGNAGFNEIMECCLPAEDSVKPNPGSDMNARKDYITAKYIERRYARKKHADNAAKLHSLCEAVKTRDIF
  GLLQAYADGVDLTEKIPLANGHEPDETALHLAVRSVDRTSLHIVDFLVQNSGNLDKQTGKGSTALHYCCL
  TDNAECLKLLLRGKASIEIANESGETPLDIAKRLKHEHCEELLTQALSGRFNSHVHVEYEWRLLHEDLDE
  SDDDMDEKLQPSPNRREDRPISFYQLGSNQLQSNAVSLARDAANLAKEKQRAFMPSILQNETYGALLSGS
  PPPAQPAAPSTTSAPPLPPRNVGKDPLTPTPPPPVAKTPSVMEALSQPSKPAPPGISQIRPPPLPPQPPS
  RLPQKKPAPGADKSTPLTNKGQPRGPVDLSATEALGPLSNAMVLQPPAPMPRKSQATKLKPKRVKALYNC
  VADNPDELTFSEGDVIIVDGEEDQEWWIGHIDGDPGRKGAFPVSFVHFIAD
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001135191
• ORF Size: 2883 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_001135191.1, NP_001128663.1
• RefSeq Size: 5589
• RefSeq ORF: 2886
• Locus ID: 8853
• Protein Pathways: Endocytosis, Fc gamma R-mediated phagocytosis
• Gene Summary: This gene encodes a multidomain protein containing an N-terminal alpha-helical region with a coiled-coil motif, followed by a pleckstrin homology (PH) domain, an Arf-GAP domain, an ankyrin homology region, a proline-rich region, and a C-terminal Src homology 3 (SH3) domain. The protein localizes in the Golgi apparatus and at the plasma membrane, where it colocalizes with protein tyrosine kinase 2-beta (PYK2). The encoded protein forms a stable complex with PYK2 in vivo. This interaction appears to be mediated by binding of its SH3 domain to the C-terminal proline-rich domain of PYK2. The encoded protein is tyrosine phosphorylated by activated PYK2. It has catalytic activity for class I and II ArfGAPs in vitro, and can bind the class III Arf ARF6 without immediate GAP activity. The encoded protein is believed to function as an ARF GAP that controls ARF-mediated vesicle budding when recruited to Golgi membranes. In addition, it functions as a substrate and downstream target for PYK2 and SRC, a pathway that may be involved in the regulation of vesicular transport. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2008]