PAG3 (ASAP2) (NM_001135191) Human Untagged Clone

CAT#: SC325379

ASAP2 (untagged)-Human ArfGAP with SH3 domain, ankyrin repeat and PH domain 2 (ASAP2), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: ASAP2
• Synonyms: AMAP2; CENTB3; DDEF2; PAG3; PAP; Pap-alpha; SHAG1
• Vector: pCMV6 series
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_001135191, the custom clone sequence may differ by one or more nucleotides
  ATGCCGGACCAGATCTCCGTGTCGGAATTCGTGGCCGAGACCCATGAGGACTACAAGGCG
  CCCACGGCCTCCAGCTTCACCACCCGCACGGCGCAGTGCCGGAACACTGTGGCGGCCATC
  GAGGAGGCTTTGGACGTGGACCGGATGGTTCTTTACAAAATGAAGAAATCCGTGAAAGCA
  ATCAACAGCTCTGGGCTGGCTCACGTGGAAAATGAAGAGCAGTACACCCAGGCTCTGGAG
  AAGTTTGGCGGCAACTGTGTATGCAGAGATGACCCAGATTTAGGAAGTGCGTTCCTGAAG
  TTCTCAGTGTTTACAAAGGAGTTGACAGCACTTTTCAAAAACCTGATTCAGAATATGAAC
  AACATAATCTCCTTCCCTTTGGACAGTTTGCTGAAGGGGGACCTGAAAGGAGTGAAAGGG
  GATCTGAAAAAGCCTTTTGATAAAGCTTGGAAGGACTATGAAACAAAAATAACCAAGATA
  GAAAAGGAGAAAAAGGAACACGCCAAGCTCCATGGGATGATTCGGACTGAAATAAGCGGA
  GCGGAAATTGCCGAAGAGATGGAAAAGGAGAGGCGCTTCTTCCAGCTACAGATGTGCGAG
  TATCTGCTGAAGGTCAACGAAATCAAGATTAAAAAGGGAGTAGATTTACTTCAGAATCTG
  ATCAAATACTTTCATGCCCAATGCAATTTTTTTCAGGATGGACTCAAAGCCGTGGAAAGC
  CTCAAACCTTCCATTGAAACGCTGTCTACGGATCTTCACACGATCAAACAGGCCCAGGAT
  GAAGAAAGAAGGCAGTTGATACAGCTTCGAGATATTTTGAAATCCGCATTGCAGGTTGAA
  CAGAAAGAGGACTCCCAAATTCGTCAGAGCACAGCTTATAGCTTACATCAGCCTCAGGGA
  AACAAGGAACATGGGACCGAGCGGAACGGCAGCCTCTACAAGAAGAGTGACGGGATCCGA
  AAAGTGTGGCAGAAAAGGAAATGTTCAGTTAAAAATGGTTTTCTGACCATATCCCATGGT
  ACCGCTAACCGGCCTCCTGCAAAGCTCAACCTGCTAACCTGCCAGGTGAAGACCAACCCT
  GAGGAGAAGAAGTGCTTTGACCTCATTTCACATGACAGAACTTACCACTTTCAAGCTGAA
  GATGAACAGGAATGTCAAATATGGATGTCTGTGCTGCAAAATAGCAAAGAAGAAGCTTTA
  AACAATGCATTTAAGGGGGATGACAATACTGGAGAAAATAACATCGTCCAAGAACTGACA
  AAGGAGATCATCTCAGAAGTGCAGAGGATGACGGGCAATGACGTCTGCTGTGACTGTGGG
  GCGCCAGATCCTACATGGCTTTCCACCAACCTGGGCATCCTGACCTGCATCGAGTGTTCC
  GGAATCCACCGAGAGCTGGGGGTTCATTATTCCAGGATGCAGTCCCTGACCTTAGATGTA
  CTGGGAACATCTGAGCTGCTGCTCGCCAAGAATATTGGGAATGCAGGCTTTAATGAGATC
  ATGGAATGTTGCCTACCAGCTGAGGACTCAGTCAAACCCAACCCAGGCAGCGACATGAAT
  GCAAGAAAGGACTACATCACAGCCAAGTACATCGAGAGGAGATACGCAAGGAAGAAGCAC
  GCGGATAACGCGGCGAAGCTTCACAGTCTTTGCGAGGCCGTCAAAACGAGAGATATTTTT
  GGATTGCTCCAAGCTTATGCTGATGGTGTGGATCTTACGGAAAAAATCCCACTGGCCAAC
  GGACATGAGCCGGATGAAACGGCCCTCCACCTTGCAGTCAGATCCGTGGATCGAACCTCT
  CTTCACATTGTAGACTTTTTAGTTCAGAACAGTGGGAACCTGGATAAACAGACAGGGAAA
  GGCAGCACAGCCCTGCACTACTGCTGCCTGACCGACAATGCCGAGTGCCTCAAGTTGCTC
  CTGCGGGGGAAGGCCTCCATCGAGATAGCAAACGAGTCAGGAGAGACTCCGCTGGACATT
  GCCAAGCGCCTCAAGCACGAGCACTGTGAGGAGCTGCTGACCCAAGCCTTATCTGGAAGA
  TTTAATTCTCACGTTCACGTTGAATATGAATGGCGACTACTCCACGAAGACCTGGATGAA
  AGTGATGACGACATGGATGAGAAATTGCAGCCCAGTCCCAACCGGCGGGAAGACCGGCCC
  ATCAGCTTCTACCAGCTGGGCTCCAACCAGCTTCAGTCTAACGCTGTATCTTTGGCCAGA
  GATGCTGCAAACCTTGCCAAGGAGAAGCAGAGGGCTTTCATGCCCAGCATCTTGCAGAAT
  GAGACTTACGGAGCCCTCCTGAGTGGCAGCCCACCTCCCGCCCAGCCTGCAGCCCCCAGC
  ACCACCAGCGCCCCCCCGCTTCCTCCACGGAATGTTGGCAAAGATCCCCTGACCCCCACG
  CCGCCCCCACCCGTTGCCAAGACGCCCAGCGTAATGGAAGCCTTGAGCCAGCCGAGCAAG
  CCTGCCCCGCCTGGGATCTCACAGATCAGGCCCCCACCTCTGCCCCCACAGCCGCCCAGC
  CGCCTCCCGCAGAAGAAGCCTGCGCCGGGGGCTGACAAGTCCACCCCACTGACCAACAAA
  GGCCAACCGAGAGGACCTGTGGATCTCTCTGCAACGGAAGCTCTGGGTCCTCTGTCCAAT
  GCTATGGTCCTGCAGCCCCCTGCACCCATGCCTAGGAAGTCGCAGGCAACCAAGTTGAAG
  CCTAAGCGGGTGAAAGCGCTCTATAACTGTGTGGCTGACAACCCCGATGAGCTCACCTTC
  TCCGAGGGGGATGTGATCATCGTGGACGGGGAGGAGGACCAGGAGTGGTGGATTGGCCAC
  ATTGATGGAGATCCTGGTCGCAAAGGCGCATTCCCGGTGTCATTTGTGCACTTTATCGCT
  GAC
  
• Restriction Sites: Please inquire
• ACCN: NM_001135191
• ORF Size: 2886 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.

Reference Data

• RefSeq: NM_001135191.1, NP_001128663.1
• RefSeq Size: 5589
• RefSeq ORF: 2886
• Locus ID: 8853
• Protein Pathways: Endocytosis, Fc gamma R-mediated phagocytosis
• Gene Summary: This gene encodes a multidomain protein containing an N-terminal alpha-helical region with a coiled-coil motif, followed by a pleckstrin homology (PH) domain, an Arf-GAP domain, an ankyrin homology region, a proline-rich region, and a C-terminal Src homology 3 (SH3) domain. The protein localizes in the Golgi apparatus and at the plasma membrane, where it colocalizes with protein tyrosine kinase 2-beta (PYK2). The encoded protein forms a stable complex with PYK2 in vivo. This interaction appears to be mediated by binding of its SH3 domain to the C-terminal proline-rich domain of PYK2. The encoded protein is tyrosine phosphorylated by activated PYK2. It has catalytic activity for class I and II ArfGAPs in vitro, and can bind the class III Arf ARF6 without immediate GAP activity. The encoded protein is believed to function as an ARF GAP that controls ARF-mediated vesicle budding when recruited to Golgi membranes. In addition, it functions as a substrate and downstream target for PYK2 and SRC, a pathway that may be involved in the regulation of vesicular transport. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2008]
  Transcript Variant: This variant (2) lacks an alternate in-frame exon in the 3' coding region, compared to variant 1. The resulting protein (isoform b) is lacking a 45-aa portion of the proline-rich region, compared to isoform a.