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Girdin (CCDC88A) (NM_001135597) Human Tagged ORF Clone
CAT#: RG226549
- TrueORF®
CCDC88A (GFP-tagged) - Human coiled-coil domain containing 88A (CCDC88A), transcript variant 1
"NM_001135597" in other vectors (2)
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Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Symbol | CCDC88A |
| Synonyms | APE; GIRDIN; GIV; GRDN; HkRP1; KIAA1212; PEHO; PEHOL |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG226549 representing NM_001135597
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGAACGAAATTTTTACTCCCCTTCTGGAGCAGTTCATGACCAGCCCTTTGGTCACTTGGGTTAAAA CGTTTGGACCTCTGGCCGCAGGAAATGGGACCAACCTTGATGAATATGTGGCTTTGGTGGATGGGGTATT CTTGAACCAGGTCATGCTCCAAATTAATCCTAAATTGGAGAGTCAGAGAGTAAATAAAAAAGTCAATAAT GATGCCTCACTTAGAATGCACAATCTATCCATTTTGGTGAGACAGATAAAATTTTATTACCAGGAGACTT TGCAGCAATTGATCATGATGTCGTTGCCAAATGTCTTAATCATTGGCAAAAATCCCTTTTCTGAACAAGG CACAGAAGAAGTTAAAAAACTGCTTTTACTTTTATTGGGTTGTGCAGTTCAGTGTCAGAAAAAAGAGGAA TTTATTGAAAGAATTCAAGGTTTAGATTTTGATACAAAAGCAGCGGTTGCCGCACATATTCAAGAGGTAA CTCATAATCAGGAAAATGTGTTTGACCTGCAATGGATGGAAGTGACTGATATGTCGCAGGAGGACATAGA ACCACTCTTGAAAAATATGGCATTGCATCTAAAAAGACTTATAGATGAGAGAGATGAACATTCAGAGACT ATCATAGAACTCTCTGAAGAGCGGGATGGTCTCCATTTTCTACCCCATGCCTCTTCATCTGCACAGTCAC CCTGTGGTTCTCCAGGCATGAAGCGAACAGAAAGTCGACAACATCTGTCGGTGGAACTGGCAGATGCTAA AGCCAAGATAAGAAGGCTTAGGCAGGAATTGGAGGAAAAGACTGAGCAGTTGTTGGATTGTAAACAAGAA CTTGAGCAAATGGAAATAGAACTCAAAAGGCTGCAACAAGAGAACATGAATTTGCTTTCGGATGCTCGCT CTGCCAGAATGTACCGAGATGAATTAGATGCACTTCGAGAGAAAGCAGTCAGAGTCGATAAGCTTGAAAG TGAAGTCAGCAGATATAAAGAGAGACTACATGATATTGAATTTTATAAGGCAAGAGTTGAGGAATTAAAA GAAGACAATCAAGTTTTATTAGAAACAAAAACCATGTTGGAAGACCAACTAGAGGGAACTCGTGCTCGTT CTGATAAATTACATGAATTAGAAAAAGAGAACTTACAACTGAAAGCTAAACTTCATGATATGGAAATGGA ACGAGATATGGATAGAAAAAAGATTGAAGAATTAATGGAAGAAAATATGACTTTGGAAATGGCACAGAAA CAAAGTATGGATGAATCATTACATCTTGGCTGGGAACTGGAACAGATATCCAGAACTAGTGAACTTTCCG AAGCACCCCAGAAATCCCTGGGCCATGAGGTGAATGAGTTGACATCAAGTAGATTATTGAAGCTAGAGAT GGAAAATCAAAGTTTGACAAAAACCGTAGAAGAGCTTCGGACTACTGTGGATTCTGTAGAAGGCAATGCT TCCAAAATCCTGAAAATGGAAAAAGAAAATCAAAGGCTCAGTAAAAAGGTTGAGATTCTTGAAAATGAGA TTGTTCAAGAAAAGCAAAGTCTTCAGAATTGTCAGAATTTAAGCAAGGATCTAATGAAGGAGAAAGCTCA GCTTGAAAAAACAATAGAAACACTGAGAGAAAATTCAGAGAGACAGATTAAGATACTGGAACAGGAAAAT GAACATCTGAATCAAACAGTGTCTTCCTTAAGGCAGCGGTCCCAGATAAGTGCAGAAGCAAGAGTGAAAG ACATTGAAAAAGAAAACAAAATTCTTCATGAATCTATCAAAGAAACAAGTAGCAAGCTAAGCAAGATTGA ATTTGAAAAAAGACAAATTAAAAAAGAATTGGAACATTATAAAGAAAAAGGAGAACGAGCTGAAGAACTT GAAAATGAATTGCATCATCTTGAAAAAGAAAATGAATTATTACAGAAAAAAATAACTAATTTAAAAATTA CTTGTGAAAAAATTGAGGCCTTAGAACAAGAAAATTCAGAGCTAGAAAGAGAAAATAGAAAATTAAAAAA AACATTGGATAGCTTTAAAAATCTGACCTTTCAGTTAGAATCCCTAGAAAAAGAGAATTCCCAACTTGAT GAGGAAAACTTAGAACTGCGAAGGAATGTAGAATCTTTGAAGTGTGCAAGCATGAAAATGGCTCAGCTAC AGCTAGAAAACAAAGAACTGGAAAGTGAAAAAGAGCAACTTAAGAAGGGTTTGGAGCTCCTGAAAGCATC TTTCAAGAAAACAGAACGCTTAGAAGTTAGCTACCAGGGTTTAGATATAGAAAATCAAAGACTGCAAAAA ACTTTAGAGAACAGCAATAAAAAAATCCAGCAATTAGAGAGTGAACTACAAGACTTAGAGATGGAAAATC AAACATTGCAGAAAAACCTAGAAGAACTAAAAATATCTAGCAAAAGACTAGAACAGCTGGAAAAAGAAAA TAAATCATTAGAGCAAGAGACTTCTCAACTGGAAAAGGATAAGAAACAATTGGAGAAGGAAAATAAGAGA CTCCGACAACAAGCAGAAATTAAAGATACCACATTAGAAGAAAATAATGTGAAGATTGGAAATTTGGAAA AAGAAAACAAAACCCTATCCAAAGAAATTGGTATATATAAAGAATCTTGTGTCCGTCTGAAAGAACTAGA AAAAGAAAATAAGGAGCTTGTGAAAAGAGCAACTATTGATATAAAAACGTTGGTTACACTACGTGAGGAT TTGGTGAGTGAAAAGTTGAAGACCCAACAGATGAACAATGATCTCGAAAAATTAACTCATGAGCTTGAGA AGATAGGGTTAAATAAGGAGCGACTCTTACATGATGAACAAAGTACTGATGACAGTAGGTATAAACTTTT GGAATCAAAATTAGAATCCACTCTTAAGAAGTCTCTTGAAATAAAAGAAGAAAAAATTGCTGCTTTAGAA GCTCGATTAGAAGAATCCACGAATTATAACCAGCAATTGCGCCAAGAACTTAAAACAGTGAAAAAAAATT ATGAAGCTCTCAAACAGAGACAAGATGAGGAAAGGATGGTACAGAGCTCTCCTCCAATATCTGGTGAAGA CAACAAATGGGAGCGAGAAAGTCAAGAAACGACTAGAGAACTTCTGAAAGTTAAAGACAGATTAATTGAA GTAGAAAGAAATAATGCTACACTGCAAGCAGAGAAGCAAGCGTTGAAAACTCAACTGAAGCAACTTGAGA CACAGAACAATAATTTGCAGGCTCAGATTCTTGCACTTCAGAGGCAGACAGTGTCATTACAAGAACAGAA TACCACTCTTCAAACACAGAATGCCAAGCTTCAGGTTGAAAATTCCACCCTTAATTCCCAAAGTACCTCA CTCATGAACCAGAATGCCCAACTCCTAATCCAGCAGTCTTCCTTAGAAAATGAAAATGAATCTGTAATCA AAGAGCGAGAAGACCTAAAATCTCTCTATGATTCTCTGATCAAAGATCATGAAAAGCTGGAACTTCTTCA TGAACGTCAGGCTTCAGAGTATGAATCTCTTATCTCTAAACATGGAACTCTGAAGTCTGCCCACAAAAAT CTTGAGGTGGAACATAGAGACCTTGAAGACCGTTACAATCAGTTATTAAAACAGAAAGGACAGTTGGAAG ATTTGGAAAAAATGCTCAAAGTAGAACAGGAAAAAATGCTGCTTGAAAATAAAAATCATGAAACAGTAGC TGCAGAATACAAGAAACTTTGTGGTGAAAATGATAGGCTGAATCATACCTATAGTCAACTTTTAAAAGAG ACTGAAGTTTTACAAACTGACCATAAAAATTTGAAAAGTCTTCTGAATAATTCCAAACTGGAACAAACAA GATTAGAAGCTGAATTTTCAAAACTAAAGGAACAATACCAACAATTGGATATTACATCAACCAAGCTGAA TAACCAGTGTGAGTTGCTAAGCCAACTTAAAGGAAATTTAGAAGAAGAAAATCGGCATCTACTAGATCAA ATTCAGACATTAATGCTACAGAACAGAACACTTTTGGAGCAGAATATGGAAAGCAAGGATCTTTTTCATG TTGAACAAAGACAGTACATTGATAAGTTAAATGAATTAAGACGTCAGAAGGAGAAACTAGAAGAGAAAAT TATGGATCAATACAAATTTTATGACCCATCTCCTCCTAGAAGGAGAGGCAACTGGATTACTCTAAAAATG AGAAAATTGATAAAGTCTAAGAAAGATATTAATCGGGAACGCCAGAAATCTCTAACATTAACACCCACCC GCTCAGACTCCAGTGAAGGATTTCTTCAGCTCCCTCATCAAGACAGTCAAGATAGTTCTTCAGTAGGTTC AAACTCTTTAGAAGATGGCCAGACCTTGGGGACCAAGAAAAGCAGCATGGTTGCACTGAAAAGACTGCCC TTTTTGAGGAACAGACCGAAGGATAAAGACAAAATGAAGGCCTGCTACCGTCGTTCCATGTCCATGAATG ACCTGGTGCAGTCCATGGTCCTAGCAGGACAGTGGACAGGTAGTACTGAGAATTTGGAGGTTCCTGATGA TATTTCAACGGGTAAAAGGAGAAAAGAATTGGGAGCTATGGCCTTCTCTACTACAGCCATCAACTTTTCA ACTGTCAACTCTTCTGCAGGCTTCAGATCCAAGCAGTTGGTTAATAATAAAGATACTACATCCTTTGAAG ACATAAGTCCACAAGGTGTTAGTGATGATTCTAGTACGGGATCAAGAGTTCATGCTTCAAGACCAGCCAG CCTTGATAGTGGCAGAACATCCACTAGCAATAGCAATAATAATGCTTCACTACATGAAGTCAAAGCAGGT GCAGTTAATAACCAAAGCAGGCCACAAAGCCACAGCAGTGGAGAATTTAGCCTGCTTCATGACCATGAGG CTTGGTCCAGCAGTGGTAGCAGTCCAATCCAGTACTTGAAAAGACAGACCAGATCAAGCCCAGTGCTCCA GCACAAAATATCTGAAACACTGGAGAGTCGACATCACAAGATCAAAACTGGTTCCCCTGGAAGTGAAGTT GTTACTCTACAACAGTTTTTGGAAGAAAGCAATAAGCTTACCTCAGTACAGATAAAGTCCTCAAGTCAAG AGAATCTTTTAGATGAAGTAATGAAAAGTTTGTCTGTCTCTTCTGACTTTTTGGGAAAAGACAAACCAGT TAGCTGTGGTCTGGCCAGGTCAGTAAGTGGAAAAACCCCAGGGGACTTCTATGATAGACGGACAACTAAG CCTGAGTTTTTGAGACCTGGTCCTCGAAAAACTGAAGATACCTACTTCATTAGTTCTGCGGGAAAACCTA CACCAGGCACTCAAGGAAAAATAAAATTAGTAAAAGAATCTTCTCTGTCACGACAATCAAAAGATAGTAA CCCTTATGCAACTTTACCTCGTGCAAGCAGCGTGATCTCAACTGCCGAAGGAACTACACGAAGGACAAGC ATCCATGATTTTTTGACCAAGGACAGTAGACTGCCTATATCAGTTGATTCACCACCAGCTGCTGCTGACA GCAACACCACTGCAGCATCTAATGTGGACAAAGTACAAGAAAGCAGAAATTCAAAAAGCAGGTCTAGGGA GCAACAAAGCTCC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG226549 representing NM_001135597
Red=Cloning site Green=Tags(s) MENEIFTPLLEQFMTSPLVTWVKTFGPLAAGNGTNLDEYVALVDGVFLNQVMLQINPKLESQRVNKKVNN DASLRMHNLSILVRQIKFYYQETLQQLIMMSLPNVLIIGKNPFSEQGTEEVKKLLLLLLGCAVQCQKKEE FIERIQGLDFDTKAAVAAHIQEVTHNQENVFDLQWMEVTDMSQEDIEPLLKNMALHLKRLIDERDEHSET IIELSEERDGLHFLPHASSSAQSPCGSPGMKRTESRQHLSVELADAKAKIRRLRQELEEKTEQLLDCKQE LEQMEIELKRLQQENMNLLSDARSARMYRDELDALREKAVRVDKLESEVSRYKERLHDIEFYKARVEELK EDNQVLLETKTMLEDQLEGTRARSDKLHELEKENLQLKAKLHDMEMERDMDRKKIEELMEENMTLEMAQK QSMDESLHLGWELEQISRTSELSEAPQKSLGHEVNELTSSRLLKLEMENQSLTKTVEELRTTVDSVEGNA SKILKMEKENQRLSKKVEILENEIVQEKQSLQNCQNLSKDLMKEKAQLEKTIETLRENSERQIKILEQEN EHLNQTVSSLRQRSQISAEARVKDIEKENKILHESIKETSSKLSKIEFEKRQIKKELEHYKEKGERAEEL ENELHHLEKENELLQKKITNLKITCEKIEALEQENSELERENRKLKKTLDSFKNLTFQLESLEKENSQLD EENLELRRNVESLKCASMKMAQLQLENKELESEKEQLKKGLELLKASFKKTERLEVSYQGLDIENQRLQK TLENSNKKIQQLESELQDLEMENQTLQKNLEELKISSKRLEQLEKENKSLEQETSQLEKDKKQLEKENKR LRQQAEIKDTTLEENNVKIGNLEKENKTLSKEIGIYKESCVRLKELEKENKELVKRATIDIKTLVTLRED LVSEKLKTQQMNNDLEKLTHELEKIGLNKERLLHDEQSTDDSRYKLLESKLESTLKKSLEIKEEKIAALE ARLEESTNYNQQLRQELKTVKKNYEALKQRQDEERMVQSSPPISGEDNKWERESQETTRELLKVKDRLIE VERNNATLQAEKQALKTQLKQLETQNNNLQAQILALQRQTVSLQEQNTTLQTQNAKLQVENSTLNSQSTS LMNQNAQLLIQQSSLENENESVIKEREDLKSLYDSLIKDHEKLELLHERQASEYESLISKHGTLKSAHKN LEVEHRDLEDRYNQLLKQKGQLEDLEKMLKVEQEKMLLENKNHETVAAEYKKLCGENDRLNHTYSQLLKE TEVLQTDHKNLKSLLNNSKLEQTRLEAEFSKLKEQYQQLDITSTKLNNQCELLSQLKGNLEEENRHLLDQ IQTLMLQNRTLLEQNMESKDLFHVEQRQYIDKLNELRRQKEKLEEKIMDQYKFYDPSPPRRRGNWITLKM RKLIKSKKDINRERQKSLTLTPTRSDSSEGFLQLPHQDSQDSSSVGSNSLEDGQTLGTKKSSMVALKRLP FLRNRPKDKDKMKACYRRSMSMNDLVQSMVLAGQWTGSTENLEVPDDISTGKRRKELGAMAFSTTAINFS TVNSSAGFRSKQLVNNKDTTSFEDISPQGVSDDSSTGSRVHASRPASLDSGRTSTSNSNNNASLHEVKAG AVNNQSRPQSHSSGEFSLLHDHEAWSSSGSSPIQYLKRQTRSSPVLQHKISETLESRHHKIKTGSPGSEV VTLQQFLEESNKLTSVQIKSSSQENLLDEVMKSLSVSSDFLGKDKPVSCGLARSVSGKTPGDFYDRRTTK PEFLRPGPRKTEDTYFISSAGKPTPGTQGKIKLVKESSLSRQSKDSNPYATLPRASSVISTAEGTTRRTS IHDFLTKDSRLPISVDSPPAAADSNTTAASNVDKVQESRNSKSRSREQQSS TRTRRLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001135597 |
| ORF Size | 5613 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Reference Data | |
| RefSeq | NM_001135597.1, NP_001129069.1 |
| RefSeq Size | 9827 |
| RefSeq ORF | 5613 |
| Locus ID | 55704 |
| Gene Summary | This gene encodes a member of the Girdin family of coiled-coil domain containing proteins. The encoded protein is an actin-binding protein that is activated by the serine/threonine kinase Akt and plays a role in cytoskeleton remodeling and cell migration. The encoded protein also enhances Akt signaling by mediating phosphoinositide 3-kinase (PI3K)-dependent activation of Akt by growth factor receptor tyrosine kinases and G protein-coupled receptors. Increased expression of this gene and phosphorylation of the encoded protein may play a role in cancer metastasis. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Dec 2011] |
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