NFYC (NM_001142589) Human Tagged ORF Clone

CAT#: RG226680

NFYC (GFP-tagged) - Human nuclear transcription factor Y, gamma (NFYC), transcript variant 4

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: NFYC
• Synonyms: CBF-C; CBFC; H1TF2A; HAP5; HSM; NF-YC
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG226680 representing NM_001142589
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCCACAGAAGGAGGATTTGGTGGTACTAGCAGCAGTGATGCCCAGCAAAGCCTACAGTCGTTCTGGC
  CTCGGGTCATGGAAGAAATCCGGAATTTAACAGTGAAAGACTTCCGAGTGCAGGAACTCCCACTGGCTCG
  TATTAAGAAGATTATGAAACTGGATGAAGATGTGAAGAGAAATGATATCGCCATGGCAATTACAAAATTT
  GATCAGTTTGATTTTCTCATCGATATTGTTCCAAGAGATGAACTGAAACCTCCAAAGCGTCAGGAGGAGG
  TGCGCCAGTCTGTAACTCCTGCCGAGCCAGTCCAGTACTATTTCACGCTGGCTCAGCAACCCACCGCTGT
  CCAAGTCCAGGGCCAGCAGCAAGGCCAGCAGACCACCAGCTCCACGACCACCATCCAGCCTGGGCAGATC
  ATCATCGCACAGCCTCAGCAGGGCCAGACCACACCTGTGACAATGCAGGTTGGAGAAGGTCAGCAGGTGC
  AGATTGTCCAGGCTCAGCCACAGGGTCAAGCCCAACAGGCCCAGAGTGGCACTGGACAGACCATGCAGGT
  GATGCAGCAGATCATCACTAACACAGGAGAGATCCAGCAGATCCCGGTGCAGCTGAATGCCGGCCAGCTG
  CAGTATATCCGCTTAGCCCAGCCTGTATCAGGCACTCAAGTTGTGCAGGGACAGATCCAGACACTTGCCA
  CCAATGCTCAACAGATTACACAGACAGAGGTCCAGCAAGGACAGCAGCAGTTCAGCCAGTTCACAGATGG
  ACAGCAGCTCTACCAGATCCAGCAAGTCACCATGCCTGCGGGCCAGGACCTCGCCCAGCCCATGTTCATC
  CAGTCAGCCAACCAGCCCTCCGACGGGCAGGCCCCCCAGGTGACCGGCGAC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG226680 representing NM_001142589
  Red=Cloning site Green=Tags(s)
  
  MSTEGGFGGTSSSDAQQSLQSFWPRVMEEIRNLTVKDFRVQELPLARIKKIMKLDEDVKRNDIAMAITKF
  DQFDFLIDIVPRDELKPPKRQEEVRQSVTPAEPVQYYFTLAQQPTAVQVQGQQQGQQTTSSTTTIQPGQI
  IIAQPQQGQTTPVTMQVGEGQQVQIVQAQPQGQAQQAQSGTGQTMQVMQQIITNTGEIQQIPVQLNAGQL
  QYIRLAQPVSGTQVVQGQIQTLATNAQQITQTEVQQGQQQFSQFTDGQQLYQIQQVTMPAGQDLAQPMFI
  QSANQPSDGQAPQVTGD
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001142589
• ORF Size: 891 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001142589.1, NP_001136061.1
• RefSeq Size: 1991 bp
• RefSeq ORF: 894 bp
• Locus ID: 4802
• Cytogenetics: 1p34.2
• Protein Families: Transcription Factors
• Protein Pathways: Antigen processing and presentation
• Gene Summary: 'This gene encodes one subunit of a trimeric complex forming a highly conserved transcription factor that binds with high specificity to CCAAT motifs in the promoters of a variety of genes. The encoded protein, subunit C, forms a tight dimer with the B subunit, a prerequisite for subunit A association. The resulting trimer binds to DNA with high specificity and affinity. Subunits B and C each contain a histone-like motif. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Dec 2008]'