OTUD5 (NM_001136159) Human Tagged ORF Clone

CAT#: RG226729

OTUD5 (GFP-tagged) - Human OTU domain containing 5 (OTUD5), transcript variant 4

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: OTUD5
• Synonyms: DUBA
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG226729 representing NM_001136159
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAAGGAGGATGGCGCCTGTCTCTTCCGGGCTGTAGCTGACCAGGTGTATGGAGACCAGGACATGCATG
  AGGTTGTGCGAAAGCATTGCATGGACTATCTGATGAAGAATGCCGACTACTTCTCCAACTATGTCACAGA
  GGACTTTACCACCTACATTAACAGGAAGCGGAAAAACAATTGCCATGGCAACCACATTGAGATGCAGGCC
  ATGGCAGAGATGTACAACCGTCCTGTGGAGGTGTACCAGTACAGCACAGAACCCATCAACACATTCCATG
  GGATACATCAAAACGAGGACGAACCCATTCGTGTTAGCTACCATCGGAATATCCACTATAATTCAGTGGT
  GAATCCTAACAAGGCCACCATTGGTGTGGGGCTGGGCCTGCCATCATTCAAACCAGGGTTTGCAGAGCAG
  TCTCTGATGAAGAATGCCATAAAAACATCGGAGGAGTCATGGATTGAACAGCAGATGCTAGAAGACAAGA
  AACGGGCCACAGACTGGGAGGCCACAAATGAAGCCATCGAGGAGCAGGTGGCTCGGGAATCCTACCTGCA
  GTGGTTGCGGGATCAGGAGAAACAGGCTCGCCAGGTCCGAGGCCCCAGCCAGCCCCGGAAAGCCAGCGCC
  ACATGCAGTTCGGCCACAGCAGCAGCCTCCAGTGGCCTGGAGGAGTGGACTAGCCGGTCCCCGCGGCAGC
  GGAGTTCAGCCTCGTCACCTGAGCACCCTGAGCTGCATGCTGAATTGGGCATGAAGCCCCCTTCCCCAGG
  CACTGTTTTAGCTCTTGCCAAACCTCCTTCGCCCTGTGCGCCAGGTACAAGCAGTCAGTTCTCGGCAGGG
  GCCGACCGGGCAACTTCCCCCCTTGTGTCCCTCTACCCTGCTTTGGAGTGCCGGGCCCTCATTCAGCAGA
  TGTCCCCCTCTGCCTTTGGTCTGAATGACTGGGATGATGATGAGATCCTAGCTTCGGTGCTGGCAGTGTC
  CCAACAGGAATACCTAGACAGTATGAAGAAAAACAAAGTGCACAGAGACCCGCCCCCAGACAAGAGT
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG226729 representing NM_001136159
  Red=Cloning site Green=Tags(s)
  
  MKEDGACLFRAVADQVYGDQDMHEVVRKHCMDYLMKNADYFSNYVTEDFTTYINRKRKNNCHGNHIEMQA
  MAEMYNRPVEVYQYSTEPINTFHGIHQNEDEPIRVSYHRNIHYNSVVNPNKATIGVGLGLPSFKPGFAEQ
  SLMKNAIKTSEESWIEQQMLEDKKRATDWEATNEAIEEQVARESYLQWLRDQEKQARQVRGPSQPRKASA
  TCSSATAAASSGLEEWTSRSPRQRSSASSPEHPELHAELGMKPPSPGTVLALAKPPSPCAPGTSSQFSAG
  ADRATSPLVSLYPALECRALIQQMSPSAFGLNDWDDDEILASVLAVSQQEYLDSMKKNKVHRDPPPDKS
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001136159
• ORF Size: 1047 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_001136159.1, NP_001129631.1
• RefSeq Size: 2426
• RefSeq ORF: 1050
• Locus ID: 55593
• Protein Families: Protease
• Protein Pathways: RIG-I-like receptor signaling pathway
• Gene Summary: This gene encodes a member of the OTU (ovarian tumor) domain-containing cysteine protease superfamily. The OTU domain confers deubiquitinase activity and the encoded protein has been shown to suppress the type I interferon-dependent innate immune response by cleaving the polyubiquitin chain from an essential type I interferon adaptor protein. Cleavage results in disassociation of the adaptor protein from a downstream signaling complex and disruption of the type I interferon signaling cascade. Alternatively spliced transcript variants encoding different isoforms have been described. [provided by RefSeq, Oct 2008]