CROT (NM_001143935) Human Tagged ORF Clone

CAT#: RG226801

CROT (GFP-tagged) - Human carnitine O-octanoyltransferase (CROT), transcript variant 1

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: CROT
• Synonyms: COT
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG226801 representing NM_001143935
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAAAATCAATTGGCTAAATCAACTGAAGAACGAACATTTCAGTACCAGGATTCTCTTCCATCACTGC
  CTGTTCCTTCACTTGAAGAATCATTAAAAAAATACCTTGAATCAGTCACCAGAACATGCTACCAGATAAG
  GGGTCTTGATCCAGATGCTAAGAGAGGGTTCTTGGATCTCACGCGGGAAGGAATTCAAGTGAAACCATTT
  GCAAATCAAGAAGAATATAAGAAAACTGAAGAAATAGTTCAAAAATTTCAAAGTGGGATTGGAGAAAAAT
  TGCACCAGAAATTGCTTGAAAGAGCAAAAGGAAAAAGAAATTGGCTGGAAGAGTGGTGGCTGAATGTTGC
  CTATCTGGATGTTCGTATACCATCACAATTGAATGTCAACTTTGCGGGTCCTGCAGCTCATTTTGAACAC
  TACTGGCCTCCAAAGGAAGGGACTCAATTAGAAAGAGGAAGTATAACTCTTTGGCATAACTTGAACTACT
  GGCAGCTATTAAGAAAAGAAAAAGTGCCTGTTCATAAAGTTGGAAATACTCCTCTAGATATGAATCAATT
  CCGAATGCTATTTTCTACCTGCAAGGTTCCAGGAATTACTAGAGACTCCATTATGAATTATTTTAGGACT
  GAGAGTGAAGGGCGTTCCCCAAACCACATTGTAGTGCTGTGTCGAGGCCGAGCTTTTGTCTTTGATGTAA
  TACATGAAGGATGTTTGGTCACCCCGCCAGAGCTTCTCAGACAACTGACATATATCCACAAGAAGTGCCA
  TAGTGAACCTGATGGACCTGGGATTGCAGCATTAACTAGTGAGGAGCGAACTCGATGGGCTAAGGCACGA
  GAATATCTGATTGGTCTTGATCCAGAGAACTTGGCTTTGTTAGAAAAAATTCAGAGTAGTTTACTGGTAT
  ATTCCATGGAGGATAGCAGTCCACATGTAACACCAGAGGATTATTCTGAGATTATTGCAGCCATCCTTAT
  TGGAGATCCAACAGTACGCTGGGGTGACAAATCCTATAACTTGATTTCCTTTTCTAATGGAGTATTTGGC
  TGTAATTGTGATCATGCTCCTTTTGATGCAATGATTATGGTGAACATCAGTTATTATGTGGATGAGAAAA
  TTTTTCAGAATGAAGGAAGATGGAAGGGTTCAGAGAAGGTACGAGATATACCACTTCCAGAAGAGCTCAT
  TTTCATTGTGGATGAGAAAGTTTTAAATGACATCAACCAAGCTAAAGCCCAGTATCTCAGGGAGGCATCT
  GATCTACAGATTGCGGCTTATGCCTTTACATCTTTTGGCAAAAAGCTAACCAAGAACAAGATGCTTCACC
  CGGATACGTTTATTCAGCTTGCACTTCAGCTGGCCTATTACAGACTTCATGGACACCCTGGTTGTTGCTA
  TGAAACAGCTATGACAAGACATTTTTATCATGGCCGTACAGAGACTATGCGATCATGCACAGTTGAAGCA
  GTGAGGTGGTGCCAGTCCATGCAGGATCCTTCTGTCAATCTTCGTGAGCGGCAGCAAAAGATGTTACAAG
  CTTTTGCAAAGCATAATAAAATGATGAAAGATTGTTCAGCTGGAAAAGGATTTGATCGTCACCTTTTAGG
  TCTCTTACTCATAGCAAAAGAGGAAGGTCTTCCTGTTCCAGAACTCTTTACGGACCCACTTTTTTCCAAA
  AGCGGAGGAGGTGGAAATTTTGTTCTCTCAACAAGTCTGGTTGGCTATTTACGAGTCCAGGGAGTGGTAG
  TTCCCATGGTACACAATGGTTATGGATTTTTCTACCATATCAGAGATGACAGGTTTGTTGTGGCCTGTTC
  AGCCTGGAAATCCTGTCCCGAGACTGATGCGGAAAAGCTAGTTCAGCTGACTTTTTGTGCTTTTCATGAT
  ATGATACAGCTGATGAACTCTACTCATCTT
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG226801 representing NM_001143935
  Red=Cloning site Green=Tags(s)
  
  MENQLAKSTEERTFQYQDSLPSLPVPSLEESLKKYLESVTRTCYQIRGLDPDAKRGFLDLTREGIQVKPF
  ANQEEYKKTEEIVQKFQSGIGEKLHQKLLERAKGKRNWLEEWWLNVAYLDVRIPSQLNVNFAGPAAHFEH
  YWPPKEGTQLERGSITLWHNLNYWQLLRKEKVPVHKVGNTPLDMNQFRMLFSTCKVPGITRDSIMNYFRT
  ESEGRSPNHIVVLCRGRAFVFDVIHEGCLVTPPELLRQLTYIHKKCHSEPDGPGIAALTSEERTRWAKAR
  EYLIGLDPENLALLEKIQSSLLVYSMEDSSPHVTPEDYSEIIAAILIGDPTVRWGDKSYNLISFSNGVFG
  CNCDHAPFDAMIMVNISYYVDEKIFQNEGRWKGSEKVRDIPLPEELIFIVDEKVLNDINQAKAQYLREAS
  DLQIAAYAFTSFGKKLTKNKMLHPDTFIQLALQLAYYRLHGHPGCCYETAMTRHFYHGRTETMRSCTVEA
  VRWCQSMQDPSVNLRERQQKMLQAFAKHNKMMKDCSAGKGFDRHLLGLLLIAKEEGLPVPELFTDPLFSK
  SGGGGNFVLSTSLVGYLRVQGVVVPMVHNGYGFFYHIRDDRFVVACSAWKSCPETDAEKLVQLTFCAFHD
  MIQLMNSTHL
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001143935
• ORF Size: 1920 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_001143935.1, NP_001137407.1
• RefSeq Size: 3342
• RefSeq ORF: 1923
• Locus ID: 54677
• Protein Families: Druggable Genome
• Gene Summary: This gene encodes a member of the carnitine/choline acetyltransferase family. The encoded protein converts 4,8-dimethylnonanoyl-CoA to its corresponding carnitine ester. This transesterification occurs in the peroxisome and is necessary for transport of medium- and long- chain acyl-CoA molecules out of the peroxisome to the cytosol and mitochondria. The protein thus plays a role in lipid metabolism and fatty acid beta-oxidation. Alternatively spliced transcript variants have been described. [provided by RefSeq, Jan 2009]