XPC (NM_001145769) Human Tagged ORF Clone

CAT#: RG226839

XPC (GFP-tagged) - Human xeroderma pigmentosum, complementation group C (XPC), transcript variant 2

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: XPC
• Synonyms: RAD4; XP3; XPCC
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG226839 representing NM_001145769
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCTCGGAAACGCGCGGCCGGCGGGGAGCCGCGGGGACGCGAACTGCGCAGCCAGAAATCCAAGGCCA
  AGAGCAAGGCCCGGCGTGAGGAGGAGGAGGAGGATGCCTTTGAAGATGAGAAACCCCCAAAGAAGAGCCT
  TCTCTCCAAAGTTTCACAAGGAAAGAGGAAAAGAGGCTGCAGTCATCCTGGGGGTTCAGCAGATGGTCCA
  GCAAAAAAGAAAGTGGCCAAGGTGACTGTTAAATCTGAAAACCTCAAGGTTATAAAGGATGAAGCCCTCA
  GCGATGGGGATGACCTCAGGGACTTTCCAAGTGACCTCAAGAAGGCACACCATCTGAAGAGAGGGGCTAC
  CATGAATGAAGACAGCAATGAAGAAGAGGAAGAAAGTGAAAATGATTGGGAAGAGGCGAAGACAAGAGAA
  AGAAGTGAAAAGATAAAACTGGAGTTTGAGACATATCTTCGGAGGGCGATGAAACGTTTCAATAAAGGGG
  TCCATGAGGACACACACAAGGTTCACCTTCTCTGCCTGCTAGCAAATGGCTTCTATCGAAATAACATCTG
  CAGCCAGCCAGATCTGCATGCTATTGGCCTGTCCATCATCCCAGCCCGCTTTACCAGAGTGCTGCCTCGA
  GATGTGGACACCTACTACCTCTCAAACCTGGTGAAGTGGTTCATTGGAACATTTACAGTTAATGCAGAAC
  TTTCAGCCAGTGAACAAGATAACCTGCAGACTACATTGGAAAGGAGATTTGCTATTTACTCTGCTCGAGA
  TGATGAGGAATTGGTCCATATATTCTTACTGATTCTCCGGGCTCTGCAGCTCTTGACCCGGCTGGTATTG
  TCTCTACAGCCAATTCCTCTGAAGTCAGCAACAGCAAAGGGAAAGAAACCTTCCAAGGAAAGATTGACTG
  CGGATCCAGGAGGCTCCTCAGAAACTTCCAGCCAAGTTCTAGAAAACCACACCAAACCAAAGACCAGCAA
  AGGAACCAAACAAGAGGAAACCTTTGCTAAGGGCACCTGCAGGCCAAGTGCCAAAGGGAAGAGGAACAAG
  GGAGGCAGAAAGAAACGGAGCAAGCCCTCCTCCAGCGAGGAAGATGAGGGCCCAGGAGACAAGCAGGAGA
  AGGCAACCCAGCGACGTCCGCATGGCCGGGAGCGGCGGGTGGCCTCCAGGGTGTCTTATAAAGAGGAGAG
  TGGGAGTGATGAGGCTGGCAGCGGCTCTGATTTTGAGCTCTCCAGTGGAGAAGCCTCTGATCCCTCTGAT
  GAGGATTCCGAACCTGGCCCTCCAAAGCAGAGGAAAGCCCCCGCTCCTCAGAGGACAAAGGCTGGGTCCA
  AGAGTGCCTCCAGGACCCATCGTGGGAGCCATCGTAAGGACCCAAGCTTGCCAGCGGCATCCTCAAGCTC
  TTCAAGCAGTAAAAGAGGCAAGAAAATGTGCAGCGATGGTGAGAAGGCAGAAAAAAGAAGCATAGCTGGT
  ATAGACCAGTGGCTAGAGGTGTTCTGTGAGCAGGAGGAAAAGTGGGTATGTGTAGACTGTGTGCACGGTG
  TGGTGGGCCAGCCTCTGACCTGTTACAAGTACGCCACCAAGCCCATGACCTATGTGGTGGGCATTGACAG
  TGACGGCTGGGTCCGAGATGTCACACAGAGGTACGACCCAGTCTGGATGACAGTGACCCGCAAGTGCCGG
  GTTGATGCTGAGTGGTGGGCCGAGACCTTGAGACCATACCAGAGCCCATTTATGGACAGGGAGAAGAAAG
  AAGACTTGGAGTTTCAGGCAAAACACATGGACCAGCCTTTGCCCACTGCCATTGGCTTATATAAGAACCA
  CCCTCTGTATGCCCTGAAGCGGCATCTCCTGAAATATGAGGCCATCTATCCCGAGACAGCTGCCATCCTT
  GGGTATTGTCGTGGAGAAGCGGTCTACTCCAGGGATTGTGTGCACACTCTGCATTCCAGGGACACGTGGC
  TGAAGAAAGCAAGAGTGGTGAGGCTTGGAGAAGTACCCTACAAGATGGTGAAAGGCTTTTCTAACCGTGC
  TCGGAAAGCCCGACTTGCTGAGCCCCAGCTGCGGGAAGAAAATGACCTGGGCCTGTTTGGCTACTGGCAG
  ACAGAGGAGTATCAGCCCCCAGTGGCCGTGGACGGGAAGGTGCCCCGGAACGAGTTTGGGAATGTGTACC
  TCTTCCTGCCCAGCATGATGCCTATTGGCTGTGTCCAGCTGAACCTGCCCAATCTACACCGCGTGGCCCG
  CAAGCTGGACATCGACTGTGTCCAGGCCATCACTGGCTTTGATTTCCATGGCGGCTACTCCCATCCCGTG
  ACTGATGGATACATCGTCTGCGAGGAATTCAAAGACGTGCTCCTGACTGCCTGGGAAAATGAGCAGGCAG
  TCATTGAAAGGAAGGAGAAGGAGAAAAAGGAGAAGCGGGCTCTAGGGAACTGGAAGTTGCTGGCCAAAGG
  TCTGCTCATCAGGGAGAGGCTGAAGCGTCGCTACGGGCCCAAGAGTGAGGCAGCAGCTCCCCACACAGAT
  GCAGGAGGTGGACTCTCTTCTGATGAAGAGGAGGGGACCAGCTCTCAAGCAGAAGCGGCCAGGATACTGG
  CTGCCTCCTGGCCTCAAAACCGAGAAGATGAAGAAAAGCAGAAGCTGAAGGGTGGGCCCAAGAAGACCAA
  AAGGGAAAAGAAAGCAGCAGCTTCCCACCTGTTCCCATTTGAGCAGCTG
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG226839 representing NM_001145769
  Red=Cloning site Green=Tags(s)
  
  MARKRAAGGEPRGRELRSQKSKAKSKARREEEEEDAFEDEKPPKKSLLSKVSQGKRKRGCSHPGGSADGP
  AKKKVAKVTVKSENLKVIKDEALSDGDDLRDFPSDLKKAHHLKRGATMNEDSNEEEEESENDWEEAKTRE
  RSEKIKLEFETYLRRAMKRFNKGVHEDTHKVHLLCLLANGFYRNNICSQPDLHAIGLSIIPARFTRVLPR
  DVDTYYLSNLVKWFIGTFTVNAELSASEQDNLQTTLERRFAIYSARDDEELVHIFLLILRALQLLTRLVL
  SLQPIPLKSATAKGKKPSKERLTADPGGSSETSSQVLENHTKPKTSKGTKQEETFAKGTCRPSAKGKRNK
  GGRKKRSKPSSSEEDEGPGDKQEKATQRRPHGRERRVASRVSYKEESGSDEAGSGSDFELSSGEASDPSD
  EDSEPGPPKQRKAPAPQRTKAGSKSASRTHRGSHRKDPSLPAASSSSSSSKRGKKMCSDGEKAEKRSIAG
  IDQWLEVFCEQEEKWVCVDCVHGVVGQPLTCYKYATKPMTYVVGIDSDGWVRDVTQRYDPVWMTVTRKCR
  VDAEWWAETLRPYQSPFMDREKKEDLEFQAKHMDQPLPTAIGLYKNHPLYALKRHLLKYEAIYPETAAIL
  GYCRGEAVYSRDCVHTLHSRDTWLKKARVVRLGEVPYKMVKGFSNRARKARLAEPQLREENDLGLFGYWQ
  TEEYQPPVAVDGKVPRNEFGNVYLFLPSMMPIGCVQLNLPNLHRVARKLDIDCVQAITGFDFHGGYSHPV
  TDGYIVCEEFKDVLLTAWENEQAVIERKEKEKKEKRALGNWKLLAKGLLIRERLKRRYGPKSEAAAPHTD
  AGGGLSSDEEEGTSSQAEAARILAASWPQNREDEEKQKLKGGPKKTKREKKAAASHLFPFEQL
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001145769
• ORF Size: 2709 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001145769.1, NP_001139241.1
• RefSeq Size: 3618 bp
• RefSeq ORF: 2712 bp
• Locus ID: 7508
• Cytogenetics: 3p25.1
• Protein Families: Druggable Genome
• Protein Pathways: Nucleotide excision repair
• Gene Summary: 'The protein encoded by this gene is a key component of the XPC complex, which plays an important role in the early steps of global genome nucleotide excision repair (NER). The encoded protein is important for damage sensing and DNA binding, and shows a preference for single-stranded DNA. Mutations in this gene or some other NER components can result in Xeroderma pigmentosum, a rare autosomal recessive disorder characterized by increased sensitivity to sunlight with the development of carcinomas at an early age. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Aug 2017]'