RAD54 (RAD54L) (NM_001142548) Human Tagged ORF Clone

CAT#: RG227119

RAD54L (GFP-tagged) - Human RAD54-like (S. cerevisiae) (RAD54L), transcript variant 2

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: RAD54L
• Synonyms: hHR54; HR54; hRAD54; RAD54A
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG227119 representing NM_001142548
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGGAGGAGCTTGGCTCCCAGCCAGCTGGCCAAGAGAAAACCTGAAGGCAGGTCCTGTGATGATGAAG
  ACTGGCAACCTGGCCTAGTGACTCCTAGGAAACGGAAATCCAGCAGTGAGACCCAGATCCAGGAGTGTTT
  CCTGTCTCCTTTTCGGAAACCTTTGAGTCAGCTAACCAATCAACCACCTTGTCTGGACAGCAGTCAGCAT
  GAAGCATTTATTCGAAGCATTTTGTCAAAGCCTTTCAAAGTCCCCATTCCAAATTATCAAGGTCCTCTGG
  GCTCTCGAGCATTGGGCCTGAAAAGGGCTGGGGTCCGCCGGGCCCTCCATGACCCCCTGGAAAAAGATGC
  CTTGGTTCTGTATGAGCCTCCCCCGCTGAGCGCTCATGACCAGCTGAAGCTTGACAAGGAGAAACTCCCT
  GTCCATGTGGTTGTTGACCCTATTCTCAGTAAGGTTTTGCGGCCTCATCAGAGAGAGGGAGTGAAATTCC
  TGTGGGAGTGTGTCACCAGTCGGCGCATCCCTGGCAGCCATGGCTGCATCATGGCTGATGAGATGGGCCT
  AGGAAAGACGCTGCAGTGCATCACATTGATGTGGACACTTTTACGCCAGAGTCCAGAGTGCAAGCCAGAA
  ATTGACAAGGCAGTGGTGGTGTCGCCTTCCAGCCTGGTGAAGAACTGGTACAATGAGGTTGGGAAATGGC
  TCGGAGGGAGGATCCAACCTCTGGCCATCGATGGAGGATCTAAGGATGAAATAGACCAAAAGCTGGAAGG
  ATTCATGAACCAGCGTGGAGCCAGGGTGTCTTCTCCCATCCTCATCATTTCCTATGAGACCTTCCGCCTT
  CATGTTGGAGTCCTCCAGAAAGGAAGTGTTGGTCTGGTCATATGTGACGAGGGACACAGGCTCAAGAACT
  CTGAGAATCAGACTTACCAAGCCCTGGACAGCTTGAACACCAGCCGGCGGGTGCTCATCTCCGGAACTCC
  CATCCAGAATGATCTGCTTGAGTATTTCAGCTTGGTACATTTTGTTAATTCCGGCATCCTAGGGACTGCC
  CATGAATTCAAGAAGCATTTTGAATTGCCAATTTTGAAGGGTCGAGACGCTGCTGCTAGTGAGGCAGACA
  GGCAGCTAGGAGAGGAGCGGCTGCGGGAGCTCACCAGCATTGTGAATAGATGCCTGATACGGAGGACTTC
  TGATATCCTTTCTAAATATCTGCCTGTGAAGATTGAGCAGGTCGTTTGTTGTAGGCTGACACCCCTTCAG
  ACTGAGTTATACAAGAGGTTTCTGAGACAAGCCAAACCGGCAGAAGAATTGCTTGAGGGCAAGATGAGTG
  TGTCTTCCCTTTCTTCCATCACCTCGCTAAAGAAGCTTTGTAATCATCCAGCTCTAATCTATGATAAGTG
  TGTGGAAGAGGAGGATGGCTTTGTGGGTGCCTTGGACCTCTTCCCTCCTGGTTACAGCTCTAAGGCCCTG
  GAGCCCCAGCTGTCAGGTAAGATGCTGGTCCTGGATTATATTCTGGCGGTGACCCGAAGCCGTAGCAGTG
  ACAAAGTAGTGCTGGTGTCGAATTACACCCAGACTTTGGATCTCTTTGAGAAGCTGTGCCGTGCCCGAAG
  GTACTTATACGTCCGCCTGGATGGCACGATGTCCATTAAGAAGCGAGCCAAGGTTGTAGAACGCTTCAAT
  AGTCCATCGAGCCCTGACTTTGTCTTCATGCTGAGCAGCAAAGCTGGGGGCTGTGGCCTCAATCTCATTG
  GGGCTAACCGGCTGGTCATGTTTGACCCTGACTGGAACCCAGCCAATGATGAACAAGCCATGGCCCGGGT
  CTGGCGAGATGGTCAAAAGAAGACTTGCTATATCTACCGCCTGCTGTCTGCAGGGACCATTGAGGAGAAG
  ATCTTCCAGCGTCAGAGCCACAAGAAGGCACTGAGCAGCTGTGTGGTGGATGAGGAGCAGGATGTAGAGC
  GCCACTTCTCTCTGGGCGAGTTGAAGGAGCTGTTTATCCTGGATGAAGCTAGCCTCAGTGACACACATGA
  CAGGTTGCACTGCCGACGTTGTGTCAACAGCCGTCAGATCCGGCCACCCCCTGATGGTTCTGACTGCACT
  TCAGACCTGGCAGGGTGGAACCACTGCACTGATAAGTGGGGGCTCCGGGATGAGGTACTCCAGGCTGCCT
  GGGATGCTGCCTCCACTGCTATCACCTTCGTCTTCCACCAGCGTTCTCATGAGGAGCAGCGGGGCCTCCG
  C
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG227119 representing NM_001142548
  Red=Cloning site Green=Tags(s)
  
  MRRSLAPSQLAKRKPEGRSCDDEDWQPGLVTPRKRKSSSETQIQECFLSPFRKPLSQLTNQPPCLDSSQH
  EAFIRSILSKPFKVPIPNYQGPLGSRALGLKRAGVRRALHDPLEKDALVLYEPPPLSAHDQLKLDKEKLP
  VHVVVDPILSKVLRPHQREGVKFLWECVTSRRIPGSHGCIMADEMGLGKTLQCITLMWTLLRQSPECKPE
  IDKAVVVSPSSLVKNWYNEVGKWLGGRIQPLAIDGGSKDEIDQKLEGFMNQRGARVSSPILIISYETFRL
  HVGVLQKGSVGLVICDEGHRLKNSENQTYQALDSLNTSRRVLISGTPIQNDLLEYFSLVHFVNSGILGTA
  HEFKKHFELPILKGRDAAASEADRQLGEERLRELTSIVNRCLIRRTSDILSKYLPVKIEQVVCCRLTPLQ
  TELYKRFLRQAKPAEELLEGKMSVSSLSSITSLKKLCNHPALIYDKCVEEEDGFVGALDLFPPGYSSKAL
  EPQLSGKMLVLDYILAVTRSRSSDKVVLVSNYTQTLDLFEKLCRARRYLYVRLDGTMSIKKRAKVVERFN
  SPSSPDFVFMLSSKAGGCGLNLIGANRLVMFDPDWNPANDEQAMARVWRDGQKKTCYIYRLLSAGTIEEK
  IFQRQSHKKALSSCVVDEEQDVERHFSLGELKELFILDEASLSDTHDRLHCRRCVNSRQIRPPPDGSDCT
  SDLAGWNHCTDKWGLRDEVLQAAWDAASTAITFVFHQRSHEEQRGLR
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001142548
• ORF Size: 2241 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_001142548.1, NP_001136020.1
• RefSeq Size: 2567
• RefSeq ORF: 2244
• Locus ID: 8438
• Protein Families: Druggable Genome, Stem cell - Pluripotency
• Protein Pathways: Homologous recombination
• Gene Summary: The protein encoded by this gene belongs to the DEAD-like helicase superfamily, and shares similarity with Saccharomyces cerevisiae Rad54, a protein known to be involved in the homologous recombination and repair of DNA. This protein has been shown to play a role in homologous recombination related repair of DNA double-strand breaks. The binding of this protein to double-strand DNA induces a DNA topological change, which is thought to facilitate homologous DNA paring, and stimulate DNA recombination. Alternative splicing results in multiple transcript variants encoding the same protein. [provided by RefSeq, Dec 2008]