Prickle (PRICKLE1) (NM_001144883) Human Tagged ORF Clone

CAT#: RG227340

PRICKLE1 (GFP-tagged) - Human prickle homolog 1 (Drosophila) (PRICKLE1), transcript variant 4

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: PRICKLE1
• Synonyms: EPM1B; RILP
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG227340 representing NM_001144883
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCCTTTGGAGATGGAGCCCAAGATGAGCAAACTGGCCTTTGGCTGTCAGAGAAGTTCCACATCAGATG
  ATGACTCTGGCTGTGCATTGGAGGAGTACGCCTGGGTCCCCCCGGGCCTGAGACCAGAGCAGATCCAGCT
  CTATTTTGCTTGCTTACCAGAGGAAAAAGTTCCTTACGTTAACAGCCCCGGAGAGAAGCATCGGATTAAA
  CAGCTTTTGTACCAGTTACCACCACATGATAATGAGGTACGGTATTGCCAGTCTTTGAGTGAAGAGGAGA
  AAAAAGAGTTGCAGGTGTTCAGTGCTCAGCGGAAGAAAGAAGCACTGGGAAGAGGAACAATTAAGCTTCT
  GTCCAGAGCAGTCATGCATGCTGTGTGTGAGCAGTGTGGTTTGAAGATAAATGGAGGTGAAGTTGCAGTG
  TTCGCCTCCCGTGCGGGCCCTGGTGTGTGCTGGCACCCATCCTGTTTTGTCTGTTTCACGTGTAATGAGC
  TGCTGGTCGACCTCATCTATTTTTATCAGGATGGAAAAATTCACTGTGGCAGGCACCATGCAGAACTGCT
  CAAACCACGGTGCTCAGCATGTGACGAGATAATTTTTGCTGATGAGTGCACAGAAGCTGAGGGTCGCCAT
  TGGCACATGAAACACTTCTGCTGCCTTGAGTGTGAAACGGTCCTGGGAGGACAGAGGTATATCATGAAGG
  ACGGCCGCCCCTTCTGCTGTGGCTGTTTTGAGTCTCTCTATGCGGAGTACTGTGAAACCTGTGGGGAACA
  TATTGGTGTGGACCATGCACAGATGACCTATGACGGGCAGCACTGGCACGCCACGGAAGCCTGCTTTTCT
  TGTGCCCAGTGTAAAGCCTCTTTGTTGGGATGTCCCTTCCTTCCCAAACAGGGTCAGATTTACTGCTCAA
  AAACGTGCAGTCTTGGTGAAGACGTCCATGCCTCTGATTCTTCCGACTCTGCATTTCAGTCAGCTCGATC
  AAGAGACTCCCGAAGAAGTGTCCGAATGGGCAAGAGCAGCCGGTCAGCAGATCAGTGTAGACAGTCTCTC
  CTCTTATCGCCTGCTCTGAACTACAAGTTTCCTGGCCTCTCAGGCAATGCTGATGACACCCTTTCTCGAA
  AATTGGATGATCTGAGTCTCTCCAGACAAGGAACAAGTTTTGCCAGTGAAGAATTTTGGAAAGGCAGAGT
  AGAGCAGGAAACTCCAGAAGACCCTGAAGAATGGGCTGATCATGAAGATTATATGACGCAGCTCCTCCTC
  AAGTTTGGTGATAAAAGCCTCTTTCAGCCACAGCCCAATGAGATGGATATTCGAGCCAGTGAGCACTGGA
  TATCTGATAACATGGTTAAAAGTAAGACCGAGTTAAAGCAAAATAACCAGAGCCTTGCAAGTAAAAAATA
  CCAGTCTGATATGTACTGGGCACAGTCACAAGATGGACTGGGCGATTCTGCTTATGGCAGCCACCCAGGC
  CCTGCAAGCAGTAGAAGGCTTCAGGAATTGGAACTGGACCATGGGGCTTCAGGGTATAATCATGATGAAA
  CACAGTGGTATGAAGATTCCCTGGAGTGTCTGTCAGACCTGAAACCAGAGCAAAGTGTTCGGGATTCGAT
  GGATTCTTTGGCATTGTCCAATATCACAGGGGCTTCGGTGGATGGAGAAAACAAGCCAAGGCCATCATTG
  TATTCTCTGCAAAATTTTGAGGAGATGGAAACAGAAGATTGTGAGAAGATGAGCAATATGGGAACTTTGA
  ACTCTTCCATGCTGCACAGGAGTGCAGAGTCCTTAAAGAGTCTAAGTTCAGAGTTGTGTCCAGAGAAAAT
  CCTGCCTGAAGAGAAGCCAGTACATCTGCCAGTGCTCAGAAGGTCCAAGTCTCAATCCAGACCCCAGCAG
  GTCAAGTTTTCTGATGATGTCATTGACAATGGGAACTATGACATTGAAATCCGGCAGCCTCCGATGAGTG
  AAAGGACTCGGAGACGCGTCTACAATTTTGAAGAGAGGGGATCCAGGTCTCATCACCACCGCCGCCGGAG
  AAGTAGAAAGTCCCGCTCCGACAATGCCCTGAATCTTGTTACAGAAAGAAAATACTCTCCCAAGGACAGA
  CTGCGGCTGTACACCCCCGATAACTATGAGAAATTTATACAGAATAAAAGTGCCCGGGAGATCCAAGCAT
  ACATCCAGAATGCTGATCTCTACGGACAGTACGCCCATGCCACTTCCGATTATGGCCTGCAGAACCCAGG
  AATGAATCGGTTTCTGGGACTCTACGGCGAGGATGATGATTCCTGGTGTTCTTCCTCCTCCTCCTCTTCC
  GACTCGGAAGAAGAAGGATATTTTCTTGGACAACCAATCCCTCAACCCCGGCCACAGAGATTTGCCTACT
  ATACAGATGACCTTTCTAGTCCACCATCTGCACTTCCCACCCCTCAGTTTGGTCAGAGGACAACAAAATC
  CAAGAAGAAAAAGGGACACAAGGGCAAAAATTGTATTATTTCT
  
  AGCGGACCGACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG227340 representing NM_001144883
  Red=Cloning site Green=Tags(s)
  
  MPLEMEPKMSKLAFGCQRSSTSDDDSGCALEEYAWVPPGLRPEQIQLYFACLPEEKVPYVNSPGEKHRIK
  QLLYQLPPHDNEVRYCQSLSEEEKKELQVFSAQRKKEALGRGTIKLLSRAVMHAVCEQCGLKINGGEVAV
  FASRAGPGVCWHPSCFVCFTCNELLVDLIYFYQDGKIHCGRHHAELLKPRCSACDEIIFADECTEAEGRH
  WHMKHFCCLECETVLGGQRYIMKDGRPFCCGCFESLYAEYCETCGEHIGVDHAQMTYDGQHWHATEACFS
  CAQCKASLLGCPFLPKQGQIYCSKTCSLGEDVHASDSSDSAFQSARSRDSRRSVRMGKSSRSADQCRQSL
  LLSPALNYKFPGLSGNADDTLSRKLDDLSLSRQGTSFASEEFWKGRVEQETPEDPEEWADHEDYMTQLLL
  KFGDKSLFQPQPNEMDIRASEHWISDNMVKSKTELKQNNQSLASKKYQSDMYWAQSQDGLGDSAYGSHPG
  PASSRRLQELELDHGASGYNHDETQWYEDSLECLSDLKPEQSVRDSMDSLALSNITGASVDGENKPRPSL
  YSLQNFEEMETEDCEKMSNMGTLNSSMLHRSAESLKSLSSELCPEKILPEEKPVHLPVLRRSKSQSRPQQ
  VKFSDDVIDNGNYDIEIRQPPMSERTRRRVYNFEERGSRSHHHRRRRSRKSRSDNALNLVTERKYSPKDR
  LRLYTPDNYEKFIQNKSAREIQAYIQNADLYGQYAHATSDYGLQNPGMNRFLGLYGEDDDSWCSSSSSSS
  DSEEEGYFLGQPIPQPRPQRFAYYTDDLSSPPSALPTPQFGQRTTKSKKKKGHKGKNCIIS
  
  SGPTRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-RsrII Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001144883
• ORF Size: 2493 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_001144883.1, NP_001138355.1
• RefSeq Size: 4250
• RefSeq ORF: 2496
• Locus ID: 144165
• Protein Families: Druggable Genome
• Protein Pathways: Wnt signaling pathway
• Gene Summary: This gene encodes a nuclear receptor that may be a negative regulator of the Wnt/beta-catenin signaling pathway. The encoded protein localizes to the nuclear membrane and has been implicated in the nuclear trafficking of the transcription repressors REST/NRSF and REST4. Mutations in this gene have been linked to progressive myoclonus epilepsy. Alternate splicing results in multiple transcript variants. A pseudogene of this gene is found on chromosome 3. [provided by RefSeq, Sep 2009]