NGLY1 (NM_001145293) Human Tagged ORF Clone
CAT#: RG227404
- TrueORF®
NGLY1 (GFP-tagged) - Human N-glycanase 1 (NGLY1), transcript variant 2
"NM_001145293" in other vectors (4)
Product Images
![](https://cdn.origene.com/img/defaults-img-expression-plasmids.jpg)
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | TurboGFP |
Symbol | NGLY1 |
Synonyms | CDDG; CDG1V; PNG1; PNGase |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RG227404 representing NM_001145293
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGGCGGCGGCATTGGGCAGCTCCTCAGGCTCGGCGTCCCCGGCCGTGGCTGAGCTCTGCCAGAACA CCCCGGAGACCTTTTTGGAGGCCTCCAAGCTGCTGCTCACCTATGCTGACAACATCCTCAGAAACCCTAA TGATGAAAAATATAGATCCATCCGGATTGGAAACACAGCCTTTTCTACTAGACTCTTGCCTGTCAGAGGA GCTGTTGAATGTTTATTTGAAATGGGCTTTGAAGAGGGAGAAACACATCTCATCTTTCCTAAAAAAGCTT CAGTGGAGCAGCTGCAAAAAATTCGTGACCTGATTGCCATAGAGAGAAGTAGCAGACTGGATGGCTCAAA TAAGAGCCACAAAGTAAAGTCATCTCAGCAACCTGCAGCCAGTACCCAGCTTCCTACAACACCATCTTCA AATCCCAGTGGGTTAAACCAGCACACAAGGAACCGTCAAGGGCAGTCATCAGATCCACCATCTGCTTCAA CGGTTGCTGCTGACTCAGCCATTCTAGAAGTTCTTCAGTCCAACATTCAGCATGTGCTGGTCTATGAAAA TCCTGCTCTTCAGGAGAAAGCGTTGGCTTGTATTCCGGTCCAAGAACTAAAAAGGAAATCACAAGAAAAG TTATCGAGAGCTAGAAAATTGGATAAAGGTATCAATATAAGTGATGAGGATTTTCTTTTGCTGGAGCTTT TGCACTGGTTTAAGGAAGAATTTTTTCACTGGGTGAATAACGTTTTGTGCAGCAAATGTGGTGGACAGAC TAGGTCTAGAGATAGATCATTACTGCCCAGTGATGATGAGCTGAAGTGGGGTGCAAAGGAAGTGGAAGAT CATTACTGTGATGCCTGCCAGTTCAGCAATCGATTCCCAAGATATAATAACCCTGAGAAACTTTTGGAAA CAAGATGTGGACGGTGTGGCGAGTGGGCCAATTGTTTTACACTGTGCTGCCGAGCTGTAGGGTTTGAAGC TCGCTATGTTTGGGATTACACAGAGTTACAGCGCACATTAAGCCTAAAACTGACCACGTTAAAGGAGATT GGAAAAACATTTTTAAGGATTTCAAAGAGACAGAAATTAATACAGGTAGTTGATGTCACTTGGCGATATT CCTGCAAACATGAAGAGGTGATTGCCAGAAGAACTAAGGTTAAAGAAGCATTACTTCGAGACACTATTAA TGGGCTTAATAAGCAGAGGCAACTGTTTTTGTCAGAAAACAGAAGGAAAGAACTTCTCCAGAGGATAATT GTGGAGCTTGTTGAATTTATATCTCCCAAAACCCCTAAACCTGGAGAACTTGGGGGAAGAATATCTGGGT CAGTGGCTTGGAGAGTAGCCCGAGGTGAAATGGGTCTACAGAGAAAAGAAACCTTGTTTATTCCCTGTGA AAATGAGAAGATTTCTAAACAGCTCCACCTTTGTTACAATATTGTGAAAGATCGTTATGTTCGAGTTTCA AATAACAATCAAACCATTTCTGGATGGGAGAATGGCGTGTGGAAAATGGAATCTATATTCAGAAAAGTTG AAACAGACTGGCACATGGTATATTTGGCCCGAAAGGAAGGATCATCTTTTGCTTATATTTCCTGGAAGTT TGAGTGTGGGTCAGTTGGCCTAAAAGTAGATAGCATTTCTATTAGAACAAGTAGTCAAACTTTTCAGACT GGAACAGTAGAATGGAAATTGCGATCTGATACAGCACAAGTAGAACTGACAGGCGATAACAGTCTTCACT CCTATGCTGATTTTTCTGGTGCCACTGAAGTTATTTTGGAAGCAGAATTAAGCAGAGGAGATGGTGATGT CGCTTGGCAACACACCCAGCTGTTTAGACAAAGCTTAAATGACCATGAAGAAAATTGTTTGGAGATAATT ATAAAATTCAGTGACCTT ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG227404 representing NM_001145293
Red=Cloning site Green=Tags(s) MAAAALGSSSGSASPAVAELCQNTPETFLEASKLLLTYADNILRNPNDEKYRSIRIGNTAFSTRLLPVRG AVECLFEMGFEEGETHLIFPKKASVEQLQKIRDLIAIERSSRLDGSNKSHKVKSSQQPAASTQLPTTPSS NPSGLNQHTRNRQGQSSDPPSASTVAADSAILEVLQSNIQHVLVYENPALQEKALACIPVQELKRKSQEK LSRARKLDKGINISDEDFLLLELLHWFKEEFFHWVNNVLCSKCGGQTRSRDRSLLPSDDELKWGAKEVED HYCDACQFSNRFPRYNNPEKLLETRCGRCGEWANCFTLCCRAVGFEARYVWDYTELQRTLSLKLTTLKEI GKTFLRISKRQKLIQVVDVTWRYSCKHEEVIARRTKVKEALLRDTINGLNKQRQLFLSENRRKELLQRII VELVEFISPKTPKPGELGGRISGSVAWRVARGEMGLQRKETLFIPCENEKISKQLHLCYNIVKDRYVRVS NNNQTISGWENGVWKMESIFRKVETDWHMVYLARKEGSSFAYISWKFECGSVGLKVDSISIRTSSQTFQT GTVEWKLRSDTAQVELTGDNSLHSYADFSGATEVILEAELSRGDGDVAWQHTQLFRQSLNDHEENCLEII IKFSDL TRTRRLE - GFP Tag - V |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_001145293 |
ORF Size | 1908 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Reference Data | |
RefSeq | NM_001145293.1, NP_001138765.1 |
RefSeq Size | 2545 |
RefSeq ORF | 1911 |
Locus ID | 55768 |
Gene Summary | This gene encodes an enzyme that catalyzes hydrolysis of an N(4)-(acetyl-beta-D-glucosaminyl) asparagine residue to N-acetyl-beta-D-glucosaminylamine and a peptide containing an aspartate residue. The encoded enzyme may play a role in the proteasome-mediated degradation of misfolded glycoproteins. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Feb 2009] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
SC326128 | NGLY1 (untagged)-Human N-glycanase 1 (NGLY1), transcript variant 2 |
USD 1,080.00 |
|
RC227404 | NGLY1 (Myc-DDK-tagged)-Human N-glycanase 1 (NGLY1), transcript variant 2 |
USD 98.00 |
|
RC227404L3 | Lenti-ORF clone of NGLY1 (Myc-DDK-tagged)-Human N-glycanase 1 (NGLY1), transcript variant 2 |
USD 620.00 |
|
RC227404L4 | Lenti-ORF clone of NGLY1 (mGFP-tagged)-Human N-glycanase 1 (NGLY1), transcript variant 2 |
USD 620.00 |
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