NGLY1 (NM_001145293) Human Untagged Clone

CAT#: SC326128

NGLY1 (untagged)-Human N-glycanase 1 (NGLY1), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: NGLY1
• Synonyms: CDDG; CDG1V; PNG1; PNGase
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_001145293, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGGCGGCGGCGGCATTGGGCAGCTCCTCAGGCTCGGCGTCCCCGGCCGTGGCTGAGCTCTGCCAGAACA
  CCCCGGAGACCTTTTTGGAGGCCTCCAAGCTGCTGCTCACCTATGCTGACAACATCCTCAGAAACCCTAA
  TGATGAAAAATATAGATCCATCCGGATTGGAAACACAGCCTTTTCTACTAGACTCTTGCCTGTCAGAGGA
  GCTGTTGAATGTTTATTTGAAATGGGCTTTGAAGAGGGAGAAACACATCTCATCTTTCCTAAAAAAGCTT
  CAGTGGAGCAGCTGCAAAAAATTCGTGACCTGATTGCCATAGAGAGAAGTAGCAGACTGGATGGCTCAAA
  TAAGAGCCACAAAGTAAAGTCATCTCAGCAACCTGCAGCCAGTACCCAGCTTCCTACAACACCATCTTCA
  AATCCCAGTGGGTTAAACCAGCACACAAGGAACCGTCAAGGGCAGTCATCAGATCCACCATCTGCTTCAA
  CGGTTGCTGCTGACTCAGCCATTCTAGAAGTTCTTCAGTCCAACATTCAGCATGTGCTGGTCTATGAAAA
  TCCTGCTCTTCAGGAGAAAGCGTTGGCTTGTATTCCGGTCCAAGAACTAAAAAGGAAATCACAAGAAAAG
  TTATCGAGAGCTAGAAAATTGGATAAAGGTATCAATATAAGTGATGAGGATTTTCTTTTGCTGGAGCTTT
  TGCACTGGTTTAAGGAAGAATTTTTTCACTGGGTGAATAACGTTTTGTGCAGCAAATGTGGTGGACAGAC
  TAGGTCTAGAGATAGATCATTACTGCCCAGTGATGATGAGCTGAAGTGGGGTGCAAAGGAAGTGGAAGAT
  CATTACTGTGATGCCTGCCAGTTCAGCAATCGATTCCCAAGATATAATAACCCTGAGAAACTTTTGGAAA
  CAAGATGTGGACGGTGTGGCGAGTGGGCCAATTGTTTTACACTGTGCTGCCGAGCTGTAGGGTTTGAAGC
  TCGCTATGTTTGGGATTACACAGAGTTACAGCGCACATTAAGCCTAAAACTGACCACGTTAAAGGAGATT
  GGAAAAACATTTTTAAGGATTTCAAAGAGACAGAAATTAATACAGGTAGTTGATGTCACTTGGCGATATT
  CCTGCAAACATGAAGAGGTGATTGCCAGAAGAACTAAGGTTAAAGAAGCATTACTTCGAGACACTATTAA
  TGGGCTTAATAAGCAGAGGCAACTGTTTTTGTCAGAAAACAGAAGGAAAGAACTTCTCCAGAGGATAATT
  GTGGAGCTTGTTGAATTTATATCTCCCAAAACCCCTAAACCTGGAGAACTTGGGGGAAGAATATCTGGGT
  CAGTGGCTTGGAGAGTAGCCCGAGGTGAAATGGGTCTACAGAGAAAAGAAACCTTGTTTATTCCCTGTGA
  AAATGAGAAGATTTCTAAACAGCTCCACCTTTGTTACAATATTGTGAAAGATCGTTATGTTCGAGTTTCA
  AATAACAATCAAACCATTTCTGGATGGGAGAATGGCGTGTGGAAAATGGAATCTATATTCAGAAAAGTTG
  AAACAGACTGGCACATGGTATATTTGGCCCGAAAGGAAGGATCATCTTTTGCTTATATTTCCTGGAAGTT
  TGAGTGTGGGTCAGTTGGCCTAAAAGTAGATAGCATTTCTATTAGAACAAGTAGTCAAACTTTTCAGACT
  GGAACAGTAGAATGGAAATTGCGATCTGATACAGCACAAGTAGAACTGACAGGCGATAACAGTCTTCACT
  CCTATGCTGATTTTTCTGGTGCCACTGAAGTTATTTTGGAAGCAGAATTAAGCAGAGGAGATGGTGATGT
  CGCTTGGCAACACACCCAGCTGTTTAGACAAAGCTTAAATGACCATGAAGAAAATTGTTTGGAGATAATT
  ATAAAATTCAGTGACCTTTGA
  
  
• Restriction Sites: SgfI-MluI
• ACCN: NM_001145293
• ORF Size: 1911 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.

Reference Data

• RefSeq: NM_001145293.1, NP_001138765.1
• RefSeq Size: 2545
• RefSeq ORF: 1911
• Locus ID: 55768
• Gene Summary: This gene encodes an enzyme that catalyzes hydrolysis of an N(4)-(acetyl-beta-D-glucosaminyl) asparagine residue to N-acetyl-beta-D-glucosaminylamine and a peptide containing an aspartate residue. The encoded enzyme may play a role in the proteasome-mediated degradation of misfolded glycoproteins. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Feb 2009]
  Transcript Variant: This variant (2) includes an alternate exon that causes a frameshift and lacks an alternate exon which restores the reading frame, compared to variant 1. The resulting protein (isoform 2) is shorter compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.