C2 (NM_001145903) Human Tagged ORF Clone

CAT#: RG227665

C2 (GFP-tagged) - Human complement component 2 (C2), transcript variant 2

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: C2
• Synonyms: ARMD14; CO2
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG227665 representing NM_001145903
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGCCCACTGATGGTTCTTTTTTGCCTGCTGTTCCTGTACCCAGCTGGCCACTGCCCCAACCCAGGCA
  TTTCACTGGGCGCAGTGCGGACAGGCTTCCGCTTTGGTCATGGGGACAAGGTCCGCTATCGCTGCTCCTC
  GAATCTTGTGCTCACGGGGTCTTCGGAGCGGGAGTGCCAGGGCAACGGGGTCTGGAGTGGAACGGAGCCC
  ATCTGCCGCCAACCCTACTCTTATGACTTCCCTGAGGACGTGGCCCCTGCCCTGGGCACTTCCTTCTCCC
  ACATGCTTGGGGCCACCAATCCCACCCAGAAGACAAAGGAAAGCCTGGGCCGTAAAATCCAAATCCAGCG
  CTCTGGTCATCTGAACCTCTACCTGCTCCTGGACTGTTCGCAGAGTGTGTCGGAAAATGACTTTCTCATC
  TTCAAGGAGAGCGCCTCCCTCATGGTGGACAGGATCTTCAGCTTTGAGATCAATGTGAGCGTTGCCATTA
  TCACCTTTGCCTCAGAGCCCAAAGTCCTCATGTCTGTCCTGAACGACAACTCCCGGGATATGACTGAGGT
  GATCAGCAGCCTGGAAAATGCCAACTATAAAGATCATGAAAATGGAACTGGGACTAACACCTATGCGGCC
  TTAAACAGTGTCTATCTCATGATGAACAACCAAATGCGACTCCTCGGCATGGAAACGATGGCCTGGCAGG
  AAATCCGACATGCCATCATCCTTCTGACAGATGGAAAGTCCAATATGGGTGGCTCTCCCAAGACAGCTGT
  TGACCATATCAGAGAGATCCTGAACATCAACCAGAAGAGGAATGACTATCTGGACATCTATGCCATCGGG
  GTGGGCAAGCTGGATGTGGACTGGAGAGAACTGAATGAGCTAGGGTCCAAGAAGGATGGTGAGAGGCATG
  CCTTCATTCTGCAGGACACAAAGGCTCTGCACCAGGTCTTTGAACATATGCTGGATGTCTCCAAGCTCAC
  AGACACCATCTGCGGGGTGGGGAACATGTCAGCAAACGCCTCTGACCAGGAGAGGACACCCTGGCATGTC
  ACTATTAAGCCCAAGAGCCAAGAGACCTGCCGGGGGGCCCTCATCTCCGACCAATGGGTCCTGACAGCAG
  CTCATTGCTTCCGCGATGGCAACGACCACTCCCTGTGGAGGGTCAATGTGGGAGACCCCAAATCCCAGTG
  GGGCAAAGAATTCCTTATTGAGAAGGCGGTGATCTCCCCAGGGTTTGATGTCTTTGCCAAAAAGAACCAG
  GGAATCCTGGAGTTCTATGGTGATGACATAGCTCTGCTGAAGCTGGCCCAGAAAGTAAAGATGTCCACCC
  ATGCCAGGCCCATCTGCCTTCCCTGCACGATGGAGGCCAATCTGGCTCTGCGGAGACCTCAAGGCAGCAC
  CTGTAGGGACCATGAGAATGAACTGCTGAACAAACAGAGTGTTCCTGCTCATTTTGTCGCCTTGAATGGG
  AGCAAACTGAACATTAACCTTAAGATGGGAGTGGAGTGGACAAGCTGTGCCGAGGTTGTCTCCCAAGAAA
  AAACCATGTTCCCCAACTTGACAGATGTCAGGGAGGTGGTGACAGACCAGTTCCTATGCAGTGGGACCCA
  GGAGGATGAGAGTCCCTGCAAGGGAGAATCTGGGGGAGCAGTTTTCCTTGAGCGGAGATTCAGGTTTTTT
  CAGGTGGGTCTGGTGAGCTGGGGTCTTTACAACCCCTGCCTTGGCTCTGCTGACAAAAACTCCCGCAAAA
  GGGCCCCTCGTAGCAAGGTCCCGCCGCCACGAGACTTTCACATCAATCTCTTCCGCATGCAGCCCTGGCT
  GAGGCAGCACCTGGGGGATGTCCTGAATTTTTTACCCCTC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG227665 representing NM_001145903
  Red=Cloning site Green=Tags(s)
  
  MGPLMVLFCLLFLYPAGHCPNPGISLGAVRTGFRFGHGDKVRYRCSSNLVLTGSSERECQGNGVWSGTEP
  ICRQPYSYDFPEDVAPALGTSFSHMLGATNPTQKTKESLGRKIQIQRSGHLNLYLLLDCSQSVSENDFLI
  FKESASLMVDRIFSFEINVSVAIITFASEPKVLMSVLNDNSRDMTEVISSLENANYKDHENGTGTNTYAA
  LNSVYLMMNNQMRLLGMETMAWQEIRHAIILLTDGKSNMGGSPKTAVDHIREILNINQKRNDYLDIYAIG
  VGKLDVDWRELNELGSKKDGERHAFILQDTKALHQVFEHMLDVSKLTDTICGVGNMSANASDQERTPWHV
  TIKPKSQETCRGALISDQWVLTAAHCFRDGNDHSLWRVNVGDPKSQWGKEFLIEKAVISPGFDVFAKKNQ
  GILEFYGDDIALLKLAQKVKMSTHARPICLPCTMEANLALRRPQGSTCRDHENELLNKQSVPAHFVALNG
  SKLNINLKMGVEWTSCAEVVSQEKTMFPNLTDVREVVTDQFLCSGTQEDESPCKGESGGAVFLERRFRFF
  QVGLVSWGLYNPCLGSADKNSRKRAPRSKVPPPRDFHINLFRMQPWLRQHLGDVLNFLPL
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001145903
• ORF Size: 1860 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001145903.1, NP_001139375.1
• RefSeq Size: 2471 bp
• RefSeq ORF: 1863 bp
• Locus ID: 717
• Cytogenetics: 6p21.33
• Protein Families: Druggable Genome, Protease, Secreted Protein
• Protein Pathways: Complement and coagulation cascades, Systemic lupus erythematosus
• Gene Summary: 'Component C2 is a serum glycoprotein that functions as part of the classical pathway of the complement system. Activated C1 cleaves C2 into C2a and C2b. The serine proteinase C2a then combines with complement factor 4b to create the C3 or C5 convertase. Deficiency of C2 has been reported to associated with certain autoimmune diseases and SNPs in this gene have been associated with altered susceptibility to age-related macular degeneration. This gene localizes within the class III region of the MHC on the short arm of chromosome 6. Alternative splicing results in multiple transcript variants encoding distinct isoforms. Additional transcript variants have been described in publications but their full-length sequence has not been determined.[provided by RefSeq, Mar 2009]'