ATP1B4 (NM_001142447) Human Tagged ORF Clone

CAT#: RG227725

ATP1B4 (GFP-tagged) - Human ATPase, Na+/K+ transporting, beta 4 polypeptide (ATP1B4), transcript variant 1

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: ATP1B4
• Synonyms: ATPase, (Na+)/K+ transporting, beta 4 polypeptide; OTTHUMP00000023940; X,K-ATPase beta-m subunit
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG227725 representing NM_001142447
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGAAGGCAACTCCGGTCCAGAAGGGCTCCATCCTTTCCTTACAGTTATCGCTACAGACTCGATGATC
  CGGATGAAGCGAACCAGAACTACTTAGCAGATGAAGAGGAGGAAGCAGAAGAAGAGGCTCGGGTGACGGT
  GGTGCCCAAATCGGAGGAGGAGGAAGAAGAGGAGGAGAAAGAAGAGGAGGAAGAGGAGGAAAAGGAGGAG
  GAAGAGGGTCAAGGTCAGCCAACAGGCAATGCCTGGTGGCAGAAATTGCAGATCATGAGTGAATACCTGT
  GGGATCCAGAGAGAAGGATGTTTCTGGCCCGAACAGGTCAGAGTTGGAGCCTGATCTTACTCATTTACTT
  CTTCTTCTATGCCTCCTTGGCTGCTGTGATCACCCTCTGCATGTACACACTATTTCTGACCATCAGTCCC
  TATATACCAACCTTCACGGAGCGGGTAAAGCCTCCTGGAGTTATGATCAGACCCTTCGCCCATAGCCTTA
  ACTTCAACTTCAACGTTTCTGAACCCGACACTTGGCAGCATTATGTGATTAGCCTAAATGGCTTTCTCCA
  GGGTTATAATGACAGTCTTCAAGAGGAAATGAATGTAGATTGTCCCCCGGGGCAGTACTTCATCCAAGAT
  GGCAATGAGGATGAGGACAAGAAGGCCTGCCAATTTAAGCGCTCCTTCCTAAAGAACTGCTCTGGTCTGG
  AGGACCCAACTTTTGGATACTCTACTGGACAGCCCTGCATCCTTCTAAAGATGAACCGGATTGTAGGCTT
  TCGTCCTGAGCTTGGAGATCCTGTGAAGGTTTCCTGCAAAGTTCAGAGAGGTGATGAAAATGACATCCGA
  TCCATCAGTTACTACCCAGAGTCGGCTTCTTTTGACCTCCGCTACTACCCTTACTACGGCAAACTGACTC
  ACGTTAACTACACATCCCCCTTGGTGGCAATGCACTTTACAGACGTGGTGAAGAACCAAGCAGTGCCTGT
  GCAGTGCCAACTGAAGGGCAAAGGCGTCATAAATGATGTCATCAATGATCGTTTTGTGGGCAGGGTAATC
  TTTACCCTGAACATAGAAACT
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG227725 representing NM_001142447
  Red=Cloning site Green=Tags(s)
  
  MRRQLRSRRAPSFPYSYRYRLDDPDEANQNYLADEEEEAEEEARVTVVPKSEEEEEEEEKEEEEEEEKEE
  EEGQGQPTGNAWWQKLQIMSEYLWDPERRMFLARTGQSWSLILLIYFFFYASLAAVITLCMYTLFLTISP
  YIPTFTERVKPPGVMIRPFAHSLNFNFNVSEPDTWQHYVISLNGFLQGYNDSLQEEMNVDCPPGQYFIQD
  GNEDEDKKACQFKRSFLKNCSGLEDPTFGYSTGQPCILLKMNRIVGFRPELGDPVKVSCKVQRGDENDIR
  SISYYPESASFDLRYYPYYGKLTHVNYTSPLVAMHFTDVVKNQAVPVQCQLKGKGVINDVINDRFVGRVI
  FTLNIET
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001142447
• ORF Size: 1071 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_001142447.2, NP_001135919.1
• RefSeq Size: 4773
• RefSeq ORF: 1074
• Locus ID: 23439
• Protein Families: Transmembrane
• Protein Pathways: Cardiac muscle contraction
• Gene Summary: This gene has been found in all vertebrate genomes sequenced to date. However, this gene has undergone a change in function in placental mammals compared to other species. Specifically, in fish, avian, and amphibian species, this gene encodes plasma membrane-bound beta-subunits of Na,K-ATPase. In placental mammals, the encoded protein interacts with the nuclear transcriptional coregulator SKIP and may be involved in the regulation of TGF-beta signaling. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Mar 2010]