NELL2 (NM_001145109) Human Tagged ORF Clone

CAT#: RG227788

NELL2 (GFP-tagged) - Human NEL-like 2 (chicken) (NELL2), transcript variant 4

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: NELL2
• Synonyms: NRP2
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG227788 representing NM_001145109
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAAACGGGACTGGGTGCTCCGCTATTTAAAGCTTGGCTGCTGATCTCAGTTTGGGGGCTTGGTGTGG
  ACCCTTCCCTACAGATTGACGTCTTAACAGAGTTAGAACTTGGGGAGTCCACGACCGGAGTGCGTCAGGT
  CCCGGGGCTGCATAATGGGACGAAAGCCTTTCTCTTTCAAGATACTCCCAGAAGCATAAAAGCATCCACT
  GCTACAGCTGAACAGTTTTTTCAGAAGCTGAGAAATAAACATGAATTTACTATTTTGGTGACCCTAAAAC
  AGACCCACTTAAATTCAGGAGTTATTCTCTCAATTCACCACTTGGATCACAGGTACCTGGAACTGGAAAG
  TAGTGGCCATCGGAATGAAGTCAGACTGCATTACCGCTCAGGCAGTCACCGCCCTCACACAGAAGTGTTT
  CCTTACATTTTGGCTGATGACAAGTGGCACAAGCTCTCCTTAGCCATCAGTGCTTCCCATTTGATTTTAC
  ACATTGACTGCAATAAAATTTATGAAAGGGTAGTAGAAAAGCCCTCCACAGACTTGCCTCTAGGCACAAC
  ATTTTGGCTAGGACAGAGAAATAATGCGCATGGATATTTTAAGGGTATAATGCAAGATGTCCAATTACTT
  GTCATGCCCCAGGGATTTATTGCTCAGTGCCCAGATCTTAATCGCACCTGTCCAACTTGCAATGACTTCC
  ATGGACTTGTGCAGAAAATCATGGAGCTACAGGATATTTTAGCCAAAACATCAGCCAAGCTGTCTCGAGC
  TGAACAGCGAATGAATAGATTGGATCAGTGCTATTGTGAAAGGACTTGCACCATGAAGGGAACCACCTAC
  CGAGAATTTGAGTCCTGGATAGACGGCTGTAAGAACTGCACATGCCTGAATGGAACCATCCAGTGTGAAA
  CTCTAATCTGCCCAAATCCTGACTGCCCACTTAAGTCGGCTCTTGCGTATGTGGATGGCAAATGCTGTAA
  GGAATGCAAATCGATATGCCAATTTCAAGGACGAACCTACTTTGAAGGAGAAAGAAATACAGTCTATTCC
  TCTTCTGGAGTATGTGTTCTCTATGAGTGCAAGGACCAGACCATGAAACTTGTTGAGAGTTCAGGCTGTC
  CAGCTTTGGATTGTCCAGAGTCTCATCAGATAACCTTGTCTCACAGCTGTTGCAAAGTTTGTAAAGGTTA
  TGACTTTTGTTCTGAAAGGCATAACTGCATGGAGAATTCCATCTGCAGAAATCTGAATGACAGGGCTGTT
  TGTAGCTGTCGAGATGGTTTTAGGGCTCTTCGAGAGGATAATGCCTACTGTGAAGACATCGATGAGTGTG
  CTGAAGGGCGCCATTACTGTCGTGAAAATACAATGTGTGTCAACACCCCGGGTTCTTTTATGTGCATCTG
  CAAAACTGGATACATCAGAATTGATGATTATTCATGTACAGAACATGATGAGTGTATCACAAATCAGCAC
  AACTGTGATGAAAATGCTTTATGCTTCAACACTGTTGGAGGACACAACTGTGTTTGCAAGCCGGGCTATA
  CAGGGAATGGAACGACATGCAAAGCATTTTGCAAAGATGGCTGTAGGAATGGAGGAGCCTGTATTGCCGC
  TAATGTGTGTGCCTGCCCACAAGGCTTCACTGGACCCAGCTGTGAAACGGACATTGATGAATGCTCTGAT
  GGTTTTGTTCAATGTGACAGTCGTGCTAATTGCATTAACCTGCCTGGATGGTACCACTGTGAGTGCAGAG
  ATGGCTACCATGACAATGGGATGTTTTCACCAAGTGGAGAATCGTGTGAAGATATTGATGAGTGTGGGAC
  CGGGAGGCACAGCTGTGCCAATGATACCATTTGCTTCAATTTGGATGGCGGATATGATTGTCGATGTCCT
  CATGGAAAGAATTGCACAGGGGACTGCATCCATGATGGAAAAGTTAAGCACAATGGTCAGATTTGGGTGT
  TGGAAAATGACAGGTGCTCTGTGTGCTCATGTCAGAATGGATTCGTTATGTGTCGACGGATGGTCTGTGA
  CTGTGAGAATCCCACAGTTGATCTTTTTTGCTGCCCTGAATGTGACCCAAGGCTTAGTAGTCAGTGCCTC
  CATCAAAATGGGGAAACTTTGTATAACAGTGGTGACACCTGGGTCCAGAATTGTCAACAGTGCCGCTGCT
  TGCAAGGGGAAGTTGATTGTTGGCCCCTGCCTTGCCCAGATGTGGAGTGTGAATTCAGCATTCTCCCAGA
  GAATGAGTGCTGCCCGCGCTGTGTCACAGACCCTTGCCAGGCTGACACCATCCGCAATGACATCACCAAG
  ACTTGCCTGGACGAAATGAATGTGGTTCGCTTCACCGGGTCCTCTTGGATCAAACATGGCACTGAGTGTA
  CTCTCTGCCAGTGCAAGAATGGCCACATCTGTTGCTCAGTGGATCCACAGTGCCTTCAGGAACTG
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG227788 representing NM_001145109
  Red=Cloning site Green=Tags(s)
  
  METGLGAPLFKAWLLISVWGLGVDPSLQIDVLTELELGESTTGVRQVPGLHNGTKAFLFQDTPRSIKAST
  ATAEQFFQKLRNKHEFTILVTLKQTHLNSGVILSIHHLDHRYLELESSGHRNEVRLHYRSGSHRPHTEVF
  PYILADDKWHKLSLAISASHLILHIDCNKIYERVVEKPSTDLPLGTTFWLGQRNNAHGYFKGIMQDVQLL
  VMPQGFIAQCPDLNRTCPTCNDFHGLVQKIMELQDILAKTSAKLSRAEQRMNRLDQCYCERTCTMKGTTY
  REFESWIDGCKNCTCLNGTIQCETLICPNPDCPLKSALAYVDGKCCKECKSICQFQGRTYFEGERNTVYS
  SSGVCVLYECKDQTMKLVESSGCPALDCPESHQITLSHSCCKVCKGYDFCSERHNCMENSICRNLNDRAV
  CSCRDGFRALREDNAYCEDIDECAEGRHYCRENTMCVNTPGSFMCICKTGYIRIDDYSCTEHDECITNQH
  NCDENALCFNTVGGHNCVCKPGYTGNGTTCKAFCKDGCRNGGACIAANVCACPQGFTGPSCETDIDECSD
  GFVQCDSRANCINLPGWYHCECRDGYHDNGMFSPSGESCEDIDECGTGRHSCANDTICFNLDGGYDCRCP
  HGKNCTGDCIHDGKVKHNGQIWVLENDRCSVCSCQNGFVMCRRMVCDCENPTVDLFCCPECDPRLSSQCL
  HQNGETLYNSGDTWVQNCQQCRCLQGEVDCWPLPCPDVECEFSILPENECCPRCVTDPCQADTIRNDITK
  TCLDEMNVVRFTGSSWIKHGTECTLCQCKNGHICCSVDPQCLQEL
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001145109
• ORF Size: 2445 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_001145109.1, NP_001138581.1
• RefSeq Size: 3210
• RefSeq ORF: 2448
• Locus ID: 4753
• Protein Families: Secreted Protein, Transmembrane
• Gene Summary: The protein encoded by this gene is a glycoprotein containing several von Willebrand factor C domains and epidermal growth factor (EGF)-like domains. The encoded protein acts as a homotrimer and is found in the cytoplasm. Several variants encoding a few different isoforms exist, and at least one isoform appears to be a secreted protein. Studies in mouse suggest that this protein plays a role in neural cell growth and differentiation as well as in oncogenesis. [provided by RefSeq, Feb 2009]