DC SIGN (CD209) (NM_001144894) Human Tagged ORF Clone

CAT#: RG227875

CD209 (GFP-tagged) - Human CD209 molecule (CD209), transcript variant 6. Note: ORF is codon optimized

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: CD209
• Synonyms: CDSIGN; CLEC4L; DC-SIGN; DC-SIGN1
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG227875 ORF sequence, codon optimized.
  Due to the complexity of NM_001144894, the ORF clone is codon optimized for mammalian Expression.
  The nucleotide sequence differs from the reference sequence, yet the amino acid sequence remains identical.
  
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCCGACAGCAAGGAGCCCAGACTGCAGCAGCTGGGTCTGCTGGTGTCAAAGGTCCCATCCTCCATAA
  GTCAGGAACAGAGCCGGCAAGACGCTATTTATCAGAATCTGACCCAGCTGAAGGCCGCAGTGGGCGAACT
  TTCCGAAAAGAGCAAGCTCCAAGAAATTTATCAGGAGCTTACTCAGCTCAAAGCAGCCGTCGGGGAGCTT
  CCAGAAAAGAGTAAACTTCAGGAGATCTATCAGGAGCTCACCAGGCTGAAAGCCGCCGTGGGAGAACTGC
  CAGAAAAAAGTAAGCTTCAAGAGATCTATCAAGAACTTACATGGCTTAAGGCAGCTGTCGGAGAGCTGCC
  AGAGAAGTCAAAGATGCAGGAGATATACCAGGAGTTGACAAGGCTGAAGGCAGCCGTGGGCGAGCTCCCC
  GAGAAGTCTAAGCAGCAGGAAATCTATCAGGAGCTGACTAGACTGAAAGCCGCGGTGGGCGAACTTCCCG
  AGAAGAGTAAACAGCAGGAAATTTACCAAGAACTGACACGCCTGAAAGCAGCGGTCGGTGAGCTCCCTGA
  GAAATCTAAACAACAAGAAATATACCAGGAACTCACTCAGCTGAAAGCCGCTGTGGAACGGCTGTGCCAT
  CCATGCCCGTGGGAATGGACATTCTTCCAGGGAAATTGTTACTTCATGAGCAACTCTCAACGAAACTGGC
  ATGATTCTATCACCGCTTGCAAAGAGGTTGGGGCCCAGCTGGTGGTAATAAAGTCCGCCGAGGAACAGAA
  TTTCCTGCAGCTGCAGTCCTCCAGATCTAACCGCTTCACTTGGATGGGGCTGAGCGACTTGAATCAGGAG
  GGGACTTGGCAGTGGGTGGACGGCAGCCCCCTGCTGCCCTCCTTTAAACAATACTGGAACAGAGGGGAAC
  CTAACAATGTGGGAGAAGAAGATTGCGCCGAGTTCAGTGGCAACGGTTGGAACGACGACAAATGCAACCT
  GGCGAAGTTTTGGATCTGCAAGAAGAGTGCCGCAAGTTGCTCCCGCGACGAAGAGCAATTCCTCAGTCCT
  GCTCCAGCCACACCCAACCCTCCCCCTGCA
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG227875 representing NM_001144894
  Red=Cloning site Green=Tags(s)
  
  MSDSKEPRLQQLGLLVSKVPSSISQEQSRQDAIYQNLTQLKAAVGELSEKSKLQEIYQELTQLKAAVGEL
  PEKSKLQEIYQELTRLKAAVGELPEKSKLQEIYQELTWLKAAVGELPEKSKMQEIYQELTRLKAAVGELP
  EKSKQQEIYQELTRLKAAVGELPEKSKQQEIYQELTRLKAAVGELPEKSKQQEIYQELTQLKAAVERLCH
  PCPWEWTFFQGNCYFMSNSQRNWHDSITACKEVGAQLVVIKSAEEQNFLQLQSSRSNRFTWMGLSDLNQE
  GTWQWVDGSPLLPSFKQYWNRGEPNNVGEEDCAEFSGNGWNDDKCNLAKFWICKKSAASCSRDEEQFLSP
  APATPNPPPA
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001144894
• ORF Size: 1080 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_001144894.1, NP_001138366.1
• RefSeq Size: 4196
• RefSeq ORF: 1083
• Locus ID: 30835
• Protein Families: Druggable Genome
• Gene Summary: This gene encodes a transmembrane receptor and is often referred to as DC-SIGN because of its expression on the surface of dendritic cells and macrophages. The encoded protein is involved in the innate immune system and recognizes numerous evolutionarily divergent pathogens ranging from parasites to viruses with a large impact on public health. The protein is organized into three distinct domains: an N-terminal transmembrane domain, a tandem-repeat neck domain and C-type lectin carbohydrate recognition domain. The extracellular region consisting of the C-type lectin and neck domains has a dual function as a pathogen recognition receptor and a cell adhesion receptor by binding carbohydrate ligands on the surface of microbes and endogenous cells. The neck region is important for homo-oligomerization which allows the receptor to bind multivalent ligands with high avidity. Variations in the number of 23 amino acid repeats in the neck domain of this protein are rare but have a significant impact on ligand binding ability. This gene is closely related in terms of both sequence and function to a neighboring gene (GeneID 10332; often referred to as L-SIGN). DC-SIGN and L-SIGN differ in their ligand-binding properties and distribution. Alternative splicing results in multiple variants. [provided by RefSeq, Feb 2009]