TLE2 (NM_001144762) Human Tagged ORF Clone

CAT#: RG227899

TLE2 (GFP-tagged) - Human transducin-like enhancer of split 2 (E(sp1) homolog, Drosophila) (TLE2), transcript variant 3

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: TLE2
• Synonyms: ESG; ESG2; GRG2
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG227899 representing NM_001144762
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCAGCGACATTATGTCATGTATTATGAGATGTCGTACGGGCTCAACATTGAAATGCATAAGCAGGCGG
  AGATTGTGAAGCGTCTGAGCGGTATCTGCGCTCAGATTATCCCCTTCCTGACCCAGGAGCATCAGCAGCA
  GGTGCTCCAGGCCGTAGAACGCGCCAAGCAGGTCACCGTGGGGGAGCTGAACAGCCTCATCGGGAGTGCA
  TCTCCCTCGCCCCCTGAGAGTCTCGTGGAGGAGGAGCGACCGAGTGGCCCTGGTGGTGGCGGGAAGCAGA
  GAGCAGATGAGAAGGAGCCATCAGGACCTTATGAAAGCGACGAAGACAAGAGTGATTACAATCTGGTGGT
  GGACGAGGACCAACCCTCAGAGCCCCCCAGCCCGGCTACCACCCCCTGCGGAAAGGTACCCATCTGCATT
  CCTGCCCGTCGGGACCTGGTGGACAGTCCAGCCTCCTTGGCCTCTAGCCTTGGCTCACCGCTGCCTAGAG
  CCAAGGAGCTCATCCTGAATGACCTTCCCGCCAGCACTCCTGCCTCCAAATCCTGTGACTCCTCCCCGCC
  CCAGGACGCTTCCACCCCCGGGCCCAGCTCGGCCAGTCACCTCTGCCAGCTTGCTGCCAAGCCAGCACCT
  TCCACGGACAGCGTCGCCCTGAGGAGCCCCCTGACTCTGTCCAGTCCCTTCACCACGTCCTTCAGCCTGG
  GCTCCCACAGCACTCTCAACGGAGACCTCTCCGTGCCCAGCTCCTACGTCAGCCTCCACCTGTCCCCCCA
  GGTCAGCAGCTCTGTGGTGTACGGACGCTCCCCCGTGATGGCATTTGAGTCTCATCCCCATCTCCGAGGG
  TCATCCGTCTCTTCCTCCCTACCCAGCATCCCTGGGGGAAAGCCGGCCTACTCCTTCCACGTGTCTGCGG
  ACGGGCAGATGCAGCCGGTTCCCTTCCCCTCGGATGCACTGGTAGGCGCGGGCATCCCGCGGCACGCCCG
  GCAGCTGCACACGCTGGCCCATGGCGAGGTGGTCTGCGCGGTCACCATCAGCGGCTCCACACAGCATGTG
  TACACGGGCGGCAAGGGCTGTGTGAAGGTGTGGGACGTGGGCCAGCCTGGGGCCAAGACGCCCGTGGCCC
  AGCTCGACTGCCTGAACCGAGACAACTACATTCGTTCCTGCAAGTTGCTGCCGGATGGCCGGAGTCTGAT
  CGTGGGCGGTGAGGCCAGCACCTTGTCCATTTGGGACCTGGCGGCGCCCACCCCCCGTATCAAGGCCGAG
  CTGACTTCCTCAGCCCCAGCCTGCTACGCCCTGGCCGTCAGCCCCGACGCCAAGGTTTGCTTCTCCTGCT
  GCAGCGATGGCAACATTGTGGTCTGGGACCTGCAGAATCAGACTATGGTCAGGCAGTTCCAGGGCCACAC
  GGACGGCGCCAGCTGCATTGATATTTCCGATTACGGCACTCGGCTCTGGACAGGGGGCCTGGACAACACG
  GTGCGCTGCTGGGACCTGCGGGAGGGCCGCCAGCTGCAGCAGCATGACTTCAGCTCCCAGATTTTCTCCC
  TGGGCCACTGCCCTAACCAGGACTGGCTGGCGGTCGGAATGGAGAGTAGCAACGTGGAGATCCTGCACGT
  CCGCAAGCCGGAGAAATACCAGCTGCACCTCCACGAGAGCTGCGTGCTGTCCCTGAAGTTTGCCTCCTGC
  GGACGGTGGTTTGTGAGCACCGGGAAGGACAACCTGCTCAACGCCTGGAGGACGCCGTACGGGGCCAGCA
  TTTTCCAGTCCAAGGAGTCGTCCTCAGTCCTGAGTTGTGACATCTCCAGAAATAACAAATACATCGTGAC
  AGGCTCGGGGGACAAGAAGGCCACCGTGTATGAGGTGGTCTAC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG227899 representing NM_001144762
  Red=Cloning site Green=Tags(s)
  
  MQRHYVMYYEMSYGLNIEMHKQAEIVKRLSGICAQIIPFLTQEHQQQVLQAVERAKQVTVGELNSLIGSA
  SPSPPESLVEEERPSGPGGGGKQRADEKEPSGPYESDEDKSDYNLVVDEDQPSEPPSPATTPCGKVPICI
  PARRDLVDSPASLASSLGSPLPRAKELILNDLPASTPASKSCDSSPPQDASTPGPSSASHLCQLAAKPAP
  STDSVALRSPLTLSSPFTTSFSLGSHSTLNGDLSVPSSYVSLHLSPQVSSSVVYGRSPVMAFESHPHLRG
  SSVSSSLPSIPGGKPAYSFHVSADGQMQPVPFPSDALVGAGIPRHARQLHTLAHGEVVCAVTISGSTQHV
  YTGGKGCVKVWDVGQPGAKTPVAQLDCLNRDNYIRSCKLLPDGRSLIVGGEASTLSIWDLAAPTPRIKAE
  LTSSAPACYALAVSPDAKVCFSCCSDGNIVVWDLQNQTMVRQFQGHTDGASCIDISDYGTRLWTGGLDNT
  VRCWDLREGRQLQQHDFSSQIFSLGHCPNQDWLAVGMESSNVEILHVRKPEKYQLHLHESCVLSLKFASC
  GRWFVSTGKDNLLNAWRTPYGASIFQSKESSSVLSCDISRNNKYIVTGSGDKKATVYEVVY
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001144762
• ORF Size: 1863 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001144762.1, NP_001138234.1
• RefSeq Size: 2299 bp
• RefSeq ORF: 1866 bp
• Locus ID: 7089
• Cytogenetics: 19p13.3
• Protein Families: Transcription Factors
• Gene Summary: ''