P2X6 (P2RX6) (NM_001159554) Human Tagged ORF Clone

CAT#: RG228328

P2RX6 (GFP-tagged) - Human purinergic receptor P2X, ligand-gated ion channel, 6 (P2RX6), transcript variant 2

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: P2RX6
• Synonyms: P2RXL1; P2X6; P2XM
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG228328 representing NM_001159554
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTGCCCGCAGCTAGCAGGAGCTGGCAGCATGGGCTCCCCAGGGGCTACGACAGGCTGGGGGCTTCTGG
  ATTATAAGACGGAGAAGTGGGCTCTCCTCGCCAAAAAAGGCTACCAGGAGCGGGACCTGGAACCCCAGTT
  TTCCATCATCACCAAACTCAAAGGGGTTTCCGTCACTCAGATCAAGGAGCTTGGAAACCGGCTGTGGGAT
  GTGGCCGACTTCGTGAAGCCACCTCAGGGAGAGAACGTGTTCTTCTTGGTGACCAACTTCCTTGTGACGC
  CAGCCCAAGTTCAGGGCAGATGCCCAGAGCACCCGTCCGTCCCACTGGCTAACTGCTGGGTCGACGAGGA
  CTGCCCCGAAGGGGAGGGAGGCACACACAGCCACGGTGTAAAAACAGGCCAGTGTGTGGTGTTCAATGGG
  ACCCACAGGACCTGTGAGATCTGGAGTTGGTGCCCCGTGGAGAGTGGCGTTGTGCCCTCGAGGCCCCTGC
  TGGCCCAGGCCCAGAACTTCACACTGTTCATCAAAAACACAGTCACCTTCAGCAAGTTCAACTTCTCTAA
  GTCCAATGCCTTGGAGACCTGGGACCCCACCTATTTTAAGCACTGCCGCTATGAACCACAATTCAGCCCC
  TACTGTCCCGTGTTCCGCATTGGGGACCTCGTGGCCAAGGCTGGAGGGACCTTCGAGGACCTGGCGTTGC
  TGGGTGGCTCTGTAGGCATCAGAGTTCACTGGGATTGTGACCTGGACACCGGGGACTCTGGCTGCTGGCC
  TCACTACTCCTTCCAGCTGCAGGAGAAGAGCTACAACTTCAGGACAGCCACTCACTGGTGGGAGCAACCG
  GGTGTGGAGGCCCGCACCCTGCTCAAGCTCTATGGAATCCGCTTCGACATCCTCGTCACCGGGCAGGCAG
  GGAAGTTCGGGCTCATCCCCACGGCCGTCACACTGGGCACCGGGGCAGCTTGGCTGGGCGTGGTCACCTT
  TTTCTGTGACCTGCTACTGCTGTATGTGGATAGAGAAGCCCATTTCTACTGGAGGACAAAGTATGAGGAG
  GCCAAGGCCCCGAAAGCAACCGCCAACTCTGTGTGGAGGGAGCTGGCCCTTGCATCCCAAGCCCGACTGG
  CCGAGTGCCTCAGACGGAGCTCAGCACCTGCACCCACGGCCACTGCTGCTGGGAGTCAGACACAGACACC
  AGGATGGCCCTGTCCAAGTTCTGACACCCACTTGCCAACCCATTCCGGGAGCCTG
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG228328 representing NM_001159554
  Red=Cloning site Green=Tags(s)
  
  MCPQLAGAGSMGSPGATTGWGLLDYKTEKWALLAKKGYQERDLEPQFSIITKLKGVSVTQIKELGNRLWD
  VADFVKPPQGENVFFLVTNFLVTPAQVQGRCPEHPSVPLANCWVDEDCPEGEGGTHSHGVKTGQCVVFNG
  THRTCEIWSWCPVESGVVPSRPLLAQAQNFTLFIKNTVTFSKFNFSKSNALETWDPTYFKHCRYEPQFSP
  YCPVFRIGDLVAKAGGTFEDLALLGGSVGIRVHWDCDLDTGDSGCWPHYSFQLQEKSYNFRTATHWWEQP
  GVEARTLLKLYGIRFDILVTGQAGKFGLIPTAVTLGTGAAWLGVVTFFCDLLLLYVDREAHFYWRTKYEE
  AKAPKATANSVWRELALASQARLAECLRRSSAPAPTATAAGSQTQTPGWPCPSSDTHLPTHSGSL
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001159554
• ORF Size: 1245 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_001159554.1, NP_001153026.1
• RefSeq Size: 2698
• RefSeq ORF: 1248
• Locus ID: 9127
• Protein Families: Druggable Genome, Ion Channels: ATP Receptors, Transmembrane
• Protein Pathways: Calcium signaling pathway, Neuroactive ligand-receptor interaction
• Gene Summary: The protein encoded by this gene belongs to the family of P2X receptors, which are ATP-gated ion channels and mediate rapid and selective permeability to cations. This gene is predominantly expressed in skeletal muscle, and regulated by p53. The encoded protein is associated with VE-cadherin at the adherens junctions of human umbilical vein endothelial cells. Alternative splicing results in multiple transcript variants. A related pseudogene, which is also located on chromosome 22, has been identified. [provided by RefSeq, Apr 2009]