SARS2 (NM_001145901) Human Tagged ORF Clone

CAT#: RG228425

SARS2 (GFP-tagged) - Human seryl-tRNA synthetase 2, mitochondrial (SARS2), nuclear gene encoding mitochondrial protein, transcript variant 1

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: SARS2
• Synonyms: mtSerRS; SARS; SARSM; SerRS; SerRSmt; SERS; SYS
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG228425 representing NM_001145901
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCTGCGTCCATGGCGCGGCGCTTGTGGCCTTTGCTGACTCGTCGGGGGTTCCGGCCCCGGGGAGGCT
  GCATCTCCAACGATAGTCCAAGGAGAAGTTTCACTACAGAGAAACGAAACCGGAACCTCCTGTACGAGTA
  TGCGCGCGAGGGCTACAGCGCACTCCCTCAGCTGGACATAGAGCGGTTCTGCGCATGCCCAGAAGAGGCC
  GCACACGCCCTGGAGCTCCGCAAGGGGGAGCTGCGCTCGGCGGACCTGCCCGCGATCATCTCGACATGGC
  AGGAGCTGAGGCAGCTGCAGGAGCAGATCCGGAGCCTGGAGGAAGAGAAGGCAGCTGTGACTGAGGCAGT
  GCGGGCCCTGCTGGCAAACCAGGACAGTGGTGAAGTGCAGCAGGTGCGGCTGGATCCAGGTGCTGGCTCC
  ATATTTGGTCCTACGTTCCTCCCATTCCCAGGCCAGCTTTCTCTCCTTGTGGAGGCCCAGCTTGAGGAGC
  AGTTCTACCTGCAGGCGCTGAAGCTGCCCAACCAGACCCACCCAGACGTGCCCGTCGGGGATGAGAGCCA
  GGCTCGAGTGCTCCACATGGTCGGAGACAAGCCAGTTTTCTCCTTCCAACCTCGGGGCCACCTGGAAATT
  GGCGAGAAACTCGACATCATCCGTCAGAAGCGCCTGTCCCACGTGTCTGGCCACCGGTCCTATTACCTGC
  GCGGGGCTGGAGCCCTCCTGCAGCACGGCCTGGTCAACTTCACATTCAACAAGCTTCTCCGCCGGGGCTT
  CACCCCCATGACGGTGCCAGACCTTCTCCGCGGAGCAGTGTTTGAAGGCTGTGGGATGACACCAAATGCC
  AACCCATCCCAAATTTACAACATCGACCCTGCCCGCTTCAAAGATCTCAACCTGGCTGGAACAGCGGAGG
  TGGGGCTTGCAGGCTACTTCATGGACCACACCGTGGCCTTCAGGGACCTGCCAGTCAGGATGGTTTGCTC
  CAGCACCTGCTACCGGGCAGAGACAAACACGGGACAGGAACCCCGGGGGCTGTATCGAGTACACCACTTC
  ACCAAGGTGGAGATGTTTGGGGTGACAGGCCCTGGGCTGGAGCAGAGCTCACAGCTGCTGGAGGAGTTCC
  TGTCCCTTCAGATGGAGATCTTGACAGAGCTGGGCTTGCACTTCCGGGTCCTGGATATGCCCACCCAAGA
  ACTGGGCCTCCCCGCCTACCGCAAGTTTGACATTGAGGCCTGGATGCCAGGCCGAGGCCGCTTTGGAGAG
  GTCACCAGTGCTTCCAACTGCACAGACTTCCAGAGCCGCCGCCTCCACATCATGTTCCAGACCGAGGCTG
  GGGAGCTGCAGTTTGCCCACACGGTGAACGCCACCGCCTGTGCTGTCCCCCGCCTTCTCATCGCGCTCCT
  GGAGAGTAACCAGCAGAAGGACGGCTCAGTGCTCGTGCCCCCTGCCCTCCAGTCCTACCTCGGCACTGAT
  CGGATCACAGCCCCTACCCACGTGCCTCTCCAGTACATCGGCCCCAACCAGCCCCGGAAGCCTGGGCTGC
  CTGGCCAGCCTGCTGTAAGC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG228425 representing NM_001145901
  Red=Cloning site Green=Tags(s)
  
  MAASMARRLWPLLTRRGFRPRGGCISNDSPRRSFTTEKRNRNLLYEYAREGYSALPQLDIERFCACPEEA
  AHALELRKGELRSADLPAIISTWQELRQLQEQIRSLEEEKAAVTEAVRALLANQDSGEVQQVRLDPGAGS
  IFGPTFLPFPGQLSLLVEAQLEEQFYLQALKLPNQTHPDVPVGDESQARVLHMVGDKPVFSFQPRGHLEI
  GEKLDIIRQKRLSHVSGHRSYYLRGAGALLQHGLVNFTFNKLLRRGFTPMTVPDLLRGAVFEGCGMTPNA
  NPSQIYNIDPARFKDLNLAGTAEVGLAGYFMDHTVAFRDLPVRMVCSSTCYRAETNTGQEPRGLYRVHHF
  TKVEMFGVTGPGLEQSSQLLEEFLSLQMEILTELGLHFRVLDMPTQELGLPAYRKFDIEAWMPGRGRFGE
  VTSASNCTDFQSRRLHIMFQTEAGELQFAHTVNATACAVPRLLIALLESNQQKDGSVLVPPALQSYLGTD
  RITAPTHVPLQYIGPNQPRKPGLPGQPAVS
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001145901
• ORF Size: 1560 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_001145901.1, NP_001139373.1
• RefSeq Size: 2083
• RefSeq ORF: 1563
• Locus ID: 54938
• Protein Pathways: Aminoacyl-tRNA biosynthesis
• Gene Summary: This gene encodes the mitochondrial seryl-tRNA synthethase precursor, a member of the class II tRNA synthetase family. The mature enzyme catalyzes the ligation of Serine to tRNA(Ser) and participates in the biosynthesis of selenocysteinyl-tRNA(sec) in mitochondria. The enzyme contains an N-terminal tRNA binding domain and a core catalytic domain. It functions in a homodimeric form, which is stabilized by tRNA binding. This gene is regulated by a bidirectional promoter that also controls the expression of mitochondrial ribosomal protein S12. Both genes are within the critical interval for the autosomal dominant deafness locus DFNA4 and might be linked to this disease. Multiple transcript variants encoding different isoforms have been identified for this gene. [provided by RefSeq, Mar 2009]