RASGRP 4 (RASGRP4) (NM_001146203) Human Tagged ORF Clone
CAT#: RG228472
- TrueORF®
RASGRP4 (GFP-tagged) - Human RAS guanyl releasing protein 4 (RASGRP4), transcript variant c
"NM_001146203" in other vectors (4)
Product Images
![](https://cdn.origene.com/img/defaults-img-expression-plasmids.jpg)
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | TurboGFP |
Symbol | RASGRP4 |
Synonyms | guanyl nucleotide releasing protein 4; RAS guanyl releasing protein 4 |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RG228472 representing NM_001146203
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAACAGAAAAGACAGTAAGAGGAAGTCCCACCAGGAATGCACCGGAAAAATAGGAGGGCGAGGCCGGC CCCGCCAAGTGCGCCGCCACAAGACATGCCCCAGCCCTCGGGAAATCAGCAAGGTCATGGCTTCCATGAA CCTGGGCCTGCTGAGTGAGGGCGGCTGCAGCGAAGATGAGCTGCTGGAGAAATGCATCCAGTCCTTCGAT TCAGCTGGCAGCCTGTGCCACGAGGACCACATGCTCAACATGGTGCTGGCCATGCACAGCTGGGTGCTGC CGTCCGCCGACCTGGCTGCCCGCCTGCTGACCTCATACCAGAAGGCCACAGGGGACACCCAGGAGCTGAG ACGGCTGCAGATCTGTCACCTGGTCAGGTACTGGCTGATGCGACACCCTGAGGTGATGCACCAGGATCCC CAGCTAGAAGAAGTCATAGGTCGTTTCTGGGCCACCGTGGCCCGGGAGGGCAACTCAGCCCAGAGAAGAC TGGGAGACTCTTCTGACCTCCTGAGCCCTGGTGGCCCTGGCCCCCCACTCCCAATGAGCAGCCCAGGCCT GGGCAAAAAGCGCAAAGTGTCCTTGCTTTTCGACCACTTGGAGACGGGGGAGCTGGCTCAGCACCTCACC TACCTGGAGTTCCGGTCCTTCCAGGCTATCACGCCCCAGGACCTGCGGAGCTACGTTTTGCAGGGCTCAG TACGAGGCTGCCCGGCCCTGGAGGGCTCCGTAGGTCTCAGCAACAGCGTGTCCCGCTGGGTGCAGGTGAT GGTGCTGAGCCGTCCCGGGCCCCTACAGCGTGCACAGGTGCTGGACAAGTTCATTCACGTGGCACAGAGG CTCCACCAGCTGCAGAATTTCAACACGCTGATGGCAGTCACAGGGGGCCTGTGTCACAGTGCCATCTCCA GACTCAAGGACTCCCATGCCCACCTGAGCCCTGACAGCACCAAGCTCTCCCTGGACCTCTTCTACACGGA AGACGAGATCTATGAGCTTTCTTATGCCCGGGAGCCGCGTTGTCCCAAGAGCCTGCCACCCTCCCCCTTC AATGCACCTCTGGTGGTGGAGTGGGCCCCTGGTGTGACACCCAAGCCGGACAGGGTCACACTGGGTCGGC ATGTGGAGCAGCTGGTGGAGTCTGTGTTCAAGAATTATGACCCTGAAGGCCGAGGAACAATCTCTCAGGA GGACTTTGAGCGACTCTCGGGCAATTTTCCCTTCGCCTGCCATGGGCTTCACCCACCCCCACGCCAGGGG AGAGGATCCTTCAGCAGAGAGGAGCTGACAGGGTACCTGCTCCGGGCCAGCGCCATCTGCTCCAAGTTGG GCCTGGCCTTCCTGCACACCTTCCATGAGGTCACCTTCCGAAAGCCTACCTTCTGCGACAGCTGCAGTGG CTTCCTCTGGGGTGTCACCAAGCAAGGCTACCGCTGTCGGGAGTGCGGGCTGTGTTGCCACAAACACTGC AGAGACCAGGTGAAGGTAGAATGTAAGAAGAGGCCAGGGGCCAAGGGCGATGCAGGACCCCCCGGAGCTC CTGTCCCATCCACACCAGCTCCCCATGCCAGCTGTGGCTCCGAGGAAAATCACTCCTACACGCTATCCCT GGAGCCTGAGACTGGGTGCCAGCTTCGCCATGCCTGGACCCAGACTGAATCCCCACACCCTTCCTGGGAA ACAGATACGGTCCCCTGCCCGGTGATGGACCCACCATCAACTGCATCCTCCAAGCTGGATTCC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG228472 representing NM_001146203
Red=Cloning site Green=Tags(s) MNRKDSKRKSHQECTGKIGGRGRPRQVRRHKTCPSPREISKVMASMNLGLLSEGGCSEDELLEKCIQSFD SAGSLCHEDHMLNMVLAMHSWVLPSADLAARLLTSYQKATGDTQELRRLQICHLVRYWLMRHPEVMHQDP QLEEVIGRFWATVAREGNSAQRRLGDSSDLLSPGGPGPPLPMSSPGLGKKRKVSLLFDHLETGELAQHLT YLEFRSFQAITPQDLRSYVLQGSVRGCPALEGSVGLSNSVSRWVQVMVLSRPGPLQRAQVLDKFIHVAQR LHQLQNFNTLMAVTGGLCHSAISRLKDSHAHLSPDSTKLSLDLFYTEDEIYELSYAREPRCPKSLPPSPF NAPLVVEWAPGVTPKPDRVTLGRHVEQLVESVFKNYDPEGRGTISQEDFERLSGNFPFACHGLHPPPRQG RGSFSREELTGYLLRASAICSKLGLAFLHTFHEVTFRKPTFCDSCSGFLWGVTKQGYRCRECGLCCHKHC RDQVKVECKKRPGAKGDAGPPGAPVPSTPAPHASCGSEENHSYTLSLEPETGCQLRHAWTQTESPHPSWE TDTVPCPVMDPPSTASSKLDS TRTRRLE - GFP Tag - V |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_001146203 |
ORF Size | 1743 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Reference Data | |
RefSeq | NM_001146203.1, NP_001139675.1 |
RefSeq Size | 2959 |
RefSeq ORF | 1746 |
Locus ID | 115727 |
Protein Families | Druggable Genome |
Protein Pathways | MAPK signaling pathway |
Gene Summary | The protein encoded by this gene is a member of the Ras guanyl nucleotide-releasing protein (RasGRP) family of Ras guanine nucleotide exchange factors. It contains a Ras exchange motif, a diacylglycerol-binding domain, and two calcium-binding EF hands. This protein was shown to activate H-Ras in a cation-dependent manner in vitro. Expression of this protein in myeloid cell lines was found to be correlated with elevated level of activated RAS protein, and the RAS activation can be greatly enhanced by phorbol ester treatment, which suggested a role of this protein in diacylglycerol regulated cell signaling pathways. Studies of a mast cell leukemia cell line expressing substantial amounts of abnormal transcripts of this gene indicated that this gene may play an important role in the final stages of mast cell development. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Apr 2009] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
SC327107 | RASGRP4 (untagged)-Human RAS guanyl releasing protein 4 (RASGRP4) transcript variant c |
USD 980.00 |
|
RC228472 | RASGRP4 (Myc-DDK-tagged)-Human RAS guanyl releasing protein 4 (RASGRP4), transcript variant c |
USD 540.00 |
|
RC228472L3 | Lenti-ORF clone of RASGRP4 (Myc-DDK-tagged)-Human RAS guanyl releasing protein 4 (RASGRP4), transcript variant c |
USD 740.00 |
|
RC228472L4 | Lenti-ORF clone of RASGRP4 (mGFP-tagged)-Human RAS guanyl releasing protein 4 (RASGRP4), transcript variant c |
USD 740.00 |
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