PDP1 (NM_001161778) Human Tagged ORF Clone

CAT#: RG228480

PDP1 (GFP-tagged) - Human pyruvate dehyrogenase phosphatase catalytic subunit 1 (PDP1), nuclear gene encoding mitochondrial protein, transcript variant 1

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: PDP1
• Synonyms: PDH; PDP; PDPC; PPM2C
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG228480 representing NM_001161778
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGCATCTCTGCCTTGCTTTCAATGGGCCGGTGCTGCTGTCGCTGCTGCTGCCCGCGCGGGTTGTGGA
  TGTTGTCGGCTCCGTGTTGTGATGACAGGAGAATGTGTGTGTGTCCCGGGCCCAGACGAATTGGAATCCC
  AGTCAGAAGTTCCAGCCTGCCACTGTTCTCTGATGCCATGCCAGCACCAACTCAACTGTTTTTTCCTCTC
  ATCCGTAACTGTGAACTGAGCAGGATCTATGGCACTGCATGTTACTGCCACCACAAACATCTCTGTTGTT
  CCTCATCGTACATTCCTCAGAGTCGACTGAGATACACACCTCATCCAGCATATGCTACCTTTTGCAGGCC
  AAAGGAGAACTGGTGGCAGTACACCCAAGGAAGGAGATATGCTTCCACACCACAGAAATTTTACCTCACA
  CCTCCACAAGTCAATAGCATCCTTAAAGCTAATGAATACAGTTTCAAAGTGCCAGAATTTGACGGCAAAA
  ATGTCAGTTCTATCCTTGGATTTGACAGCAATCAGCTGCCTGCAAATGCACCCATTGAGGACCGGAGAAG
  TGCAGCAACCTGCTTGCAGACCAGAGGGATGCTTTTGGGGGTTTTTGATGGCCATGCAGGTTGTGCTTGT
  TCCCAGGCAGTCAGTGAAAGACTCTTTTATTATATTGCTGTCTCTTTGTTACCCCATGAGACTTTGCTAG
  AGATTGAAAATGCAGTGGAGAGCGGCCGGGCACTGCTACCCATTCTCCAGTGGCACAAGCACCCCAATGA
  TTACTTTAGTAAGGAGGCATCCAAATTGTACTTTAACAGCTTGAGGACTTACTGGCAAGAGCTTATAGAC
  CTCAACACTGGTGAGTCGACTGATATTGATGTTAAGGAGGCTCTAATTAATGCCTTCAAGAGGCTTGATA
  ATGACATCTCCTTGGAGGCGCAAGTTGGTGATCCTAATTCTTTTCTCAACTACCTGGTGCTTCGAGTGGC
  ATTTTCTGGAGCCACTGCTTGTGTGGCCCATGTGGATGGTGTTGACCTTCATGTGGCCAATACTGGCGAT
  AGCAGAGCCATGCTGGGTGTGCAGGAAGAGGACGGCTCATGGTCAGCAGTCACGCTGTCTAATGACCACA
  ATGCTCAAAATGAAAGAGAACTAGAACGGCTGAAATTGGAACATCCAAAGAGTGAGGCCAAGAGTGTCGT
  GAAACAGGATCGGCTGCTTGGCTTGCTGATGCCATTTAGGGCATTTGGAGATGTAAAGTTCAAATGGAGC
  ATTGACCTTCAAAAGAGAGTGATAGAATCTGGCCCAGACCAGTTGAATGACAATGAATATACCAAGTTTA
  TTCCTCCTAATTATCACACACCTCCTTATCTCACTGCTGAGCCAGAGGTAACTTACCACCGATTAAGGCC
  ACAGGATAAGTTTCTGGTGTTGGCTACTGATGGGTTGTGGGAGACTATGCATAGGCAGGATGTGGTTAGG
  ATTGTGGGTGAGTACCTAACTGGCATGCATCACCAACAGCCAATAGCTGTTGGTGGCTACAAGGTGACTC
  TGGGACAGATGCATGGCCTTTTAACAGAAAGGAGAACCAAAATGTCCTCGGTATTTGAGGATCAGAACGC
  AGCAACCCATCTCATTCGCCACGCTGTGGGCAACAACGAGTTTGGGACTGTTGATCATGAGCGCCTCTCT
  AAAATGCTTAGTCTTCCTGAAGAGCTTGCTCGAATGTACAGAGATGACATTACAATCATTGTAGTTCAGT
  TCAATTCTCATGTTGTAGGGGCGTATCAAAACCAAGAA
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG228480 representing NM_001161778
  Red=Cloning site Green=Tags(s)
  
  MSISALLSMGRCCCRCCCPRGLWMLSAPCCDDRRMCVCPGPRRIGIPVRSSSLPLFSDAMPAPTQLFFPL
  IRNCELSRIYGTACYCHHKHLCCSSSYIPQSRLRYTPHPAYATFCRPKENWWQYTQGRRYASTPQKFYLT
  PPQVNSILKANEYSFKVPEFDGKNVSSILGFDSNQLPANAPIEDRRSAATCLQTRGMLLGVFDGHAGCAC
  SQAVSERLFYYIAVSLLPHETLLEIENAVESGRALLPILQWHKHPNDYFSKEASKLYFNSLRTYWQELID
  LNTGESTDIDVKEALINAFKRLDNDISLEAQVGDPNSFLNYLVLRVAFSGATACVAHVDGVDLHVANTGD
  SRAMLGVQEEDGSWSAVTLSNDHNAQNERELERLKLEHPKSEAKSVVKQDRLLGLLMPFRAFGDVKFKWS
  IDLQKRVIESGPDQLNDNEYTKFIPPNYHTPPYLTAEPEVTYHRLRPQDKFLVLATDGLWETMHRQDVVR
  IVGEYLTGMHHQQPIAVGGYKVTLGQMHGLLTERRTKMSSVFEDQNAATHLIRHAVGNNEFGTVDHERLS
  KMLSLPEELARMYRDDITIIVVQFNSHVVGAYQNQE
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001161778
• ORF Size: 1788 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_001161778.1, NP_001155250.1
• RefSeq Size: 4248
• RefSeq ORF: 1790
• Locus ID: 54704
• Protein Families: Druggable Genome, Phosphatase
• Gene Summary: Pyruvate dehydrogenase (E1) is one of the three components (E1, E2, and E3) of the large pyruvate dehydrogenase complex. Pyruvate dehydrogenase kinases catalyze phosphorylation of serine residues of E1 to inactivate the E1 component and inhibit the complex. Pyruvate dehydrogenase phosphatases catalyze the dephosphorylation and activation of the E1 component to reverse the effects of pyruvate dehydrogenase kinases. Pyruvate dehydrogenase phosphatase is a heterodimer consisting of catalytic and regulatory subunits. Two catalytic subunits have been reported; one is predominantly expressed in skeletal muscle and another one is is much more abundant in the liver. The catalytic subunit, encoded by this gene, is the former, and belongs to the protein phosphatase 2C (PP2C) superfamily. Along with the pyruvate dehydrogenase complex and pyruvate dehydrogenase kinases, this enzyme is located in the mitochondrial matrix. Mutation in this gene causes pyruvate dehydrogenase phosphatase deficiency. Multiple alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Jun 2009]