PDP1 (NM_001161778) Human Untagged Clone

CAT#: SC327115

PDP1 (untagged)-Human pyruvate dehyrogenase phosphatase catalytic subunit 1 (PDP1) nuclear gene encoding mitochondrial protein transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: PDP1
• Synonyms: PDH; PDP; PDPC; PPM2C
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_001161778, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGAGCATCTCTGCCTTGCTTTCAATGGGCCGGTGCTGCTGTCGCTGCTGCTGCCCGCGCGGGTTGTGGA
  TGTTGTCGGCTCCGTGTTGTGATGACAGGAGAATGTGTGTGTGTCCCGGGCCCAGACGAATTGGAATCCC
  AGTCAGAAGTTCCAGCCTGCCACTGTTCTCTGATGCCATGCCAGCACCAACTCAACTGTTTTTTCCTCTC
  ATCCGTAACTGTGAACTGAGCAGGATCTATGGCACTGCATGTTACTGCCACCACAAACATCTCTGTTGTT
  CCTCATCGTACATTCCTCAGAGTCGACTGAGATACACACCTCATCCAGCATATGCTACCTTTTGCAGGCC
  AAAGGAGAACTGGTGGCAGTACACCCAAGGAAGGAGATATGCTTCCACACCACAGAAATTTTACCTCACA
  CCTCCACAAGTCAATAGCATCCTTAAAGCTAATGAATACAGTTTCAAAGTGCCAGAATTTGACGGCAAAA
  ATGTCAGTTCTATCCTTGGATTTGACAGCAATCAGCTGCCTGCAAATGCACCCATTGAGGACCGGAGAAG
  TGCAGCAACCTGCTTGCAGACCAGAGGGATGCTTTTGGGGGTTTTTGATGGCCATGCAGGTTGTGCTTGT
  TCCCAGGCAGTCAGTGAAAGACTCTTTTATTATATTGCTGTCTCTTTGTTACCCCATGAGACTTTGCTAG
  AGATTGAAAATGCAGTGGAGAGCGGCCGGGCACTGCTACCCATTCTCCAGTGGCACAAGCACCCCAATGA
  TTACTTTAGTAAGGAGGCATCCAAATTGTACTTTAACAGCTTGAGGACTTACTGGCAAGAGCTTATAGAC
  CTCAACACTGGTGAGTCGACTGATATTGATGTTAAGGAGGCTCTAATTAATGCCTTCAAGAGGCTTGATA
  ATGACATCTCCTTGGAGGCGCAAGTTGGTGATCCTAATTCTTTTCTCAACTACCTGGTGCTTCGAGTGGC
  ATTTTCTGGAGCCACTGCTTGTGTGGCCCATGTGGATGGTGTTGACCTTCATGTGGCCAATACTGGCGAT
  AGCAGAGCCATGCTGGGTGTGCAGGAAGAGGACGGCTCATGGTCAGCAGTCACGCTGTCTAATGACCACA
  ATGCTCAAAATGAAAGAGAACTAGAACGGCTGAAATTGGAACATCCAAAGAGTGAGGCCAAGAGTGTCGT
  GAAACAGGATCGGCTGCTTGGCTTGCTGATGCCATTTAGGGCATTTGGAGATGTAAAGTTCAAATGGAGC
  ATTGACCTTCAAAAGAGAGTGATAGAATCTGGCCCAGACCAGTTGAATGACAATGAATATACCAAGTTTA
  TTCCTCCTAATTATCACACACCTCCTTATCTCACTGCTGAGCCAGAGGTAACTTACCACCGATTAAGGCC
  ACAGGATAAGTTTCTGGTGTTGGCTACTGATGGGTTGTGGGAGACTATGCATAGGCAGGATGTGGTTAGG
  ATTGTGGGTGAGTACCTAACTGGCATGCATCACCAACAGCCAATAGCTGTTGGTGGCTACAAGGTGACTC
  TGGGACAGATGCATGGCCTTTTAACAGAAAGGAGAACCAAAATGTCCTCGGTATTTGAGGATCAGAACGC
  AGCAACCCATCTCATTCGCCACGCTGTGGGCAACAACGAGTTTGGGACTGTTGATCATGAGCGCCTCTCT
  AAAATGCTTAGTCTTCCTGAAGAGCTTGCTCGAATGTACAGAGATGACATTACAATCATTGTAGTTCAGT
  TCAATTCTCATGTTGTAGGGGCGTATCAAAACCAAGAATAG
  
  
• Restriction Sites: SgfI-MluI
• ACCN: NM_001161778
• ORF Size: 1791 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.

Reference Data

• RefSeq: NM_001161778.1, NP_001155250.1
• RefSeq Size: 4248
• RefSeq ORF: 1791
• Locus ID: 54704
• Protein Families: Druggable Genome, Phosphatase
• Gene Summary: Pyruvate dehydrogenase (E1) is one of the three components (E1, E2, and E3) of the large pyruvate dehydrogenase complex. Pyruvate dehydrogenase kinases catalyze phosphorylation of serine residues of E1 to inactivate the E1 component and inhibit the complex. Pyruvate dehydrogenase phosphatases catalyze the dephosphorylation and activation of the E1 component to reverse the effects of pyruvate dehydrogenase kinases. Pyruvate dehydrogenase phosphatase is a heterodimer consisting of catalytic and regulatory subunits. Two catalytic subunits have been reported; one is predominantly expressed in skeletal muscle and another one is is much more abundant in the liver. The catalytic subunit, encoded by this gene, is the former, and belongs to the protein phosphatase 2C (PP2C) superfamily. Along with the pyruvate dehydrogenase complex and pyruvate dehydrogenase kinases, this enzyme is located in the mitochondrial matrix. Mutation in this gene causes pyruvate dehydrogenase phosphatase deficiency. Multiple alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Jun 2009]
  Transcript Variant: This variant (1) encodes the longest isoform (1).