KCNMA1 (NM_001161352) Human Tagged ORF Clone

CAT#: RG228657

KCNMA1 (GFP-tagged) - Human potassium large conductance calcium-activated channel, subfamily M, alpha member 1 (KCNMA1), transcript variant 3

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: KCNMA1
• Synonyms: bA205K10.1; BKTM; CADEDS; hSlo; KCa1.1; MaxiK; mSLO1; PNKD3; SAKCA; SLO; SLO-ALPHA; SLO1
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG228657 representing NM_001161352
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCAAATGGTGGCGGCGGCGGCGGCGGCAGCAGCGGCGGCGGCGGCGGCGGCGGAGGCAGCAGTCTTA
  GAATGAGTAGCAATATCCACGCGAACCATCTCAGCCTAGACGCGTCCTCCTCCTCCTCCTCCTCCTCTTC
  CTCTTCTTCTTCTTCCTCCTCCTCTTCCTCCTCGTCCTCGGTCCACGAGCCCAAGATGGATGCGCTCATC
  ATCCCGGTGACCATGGAGGTGCCGTGCGACAGCCGGGGCCAACGCATGTGGTGGGCTTTCCTGGCCTCCT
  CCATGGTGACTTTCTTCGGGGGCCTCTTCATCATCTTGCTCTGGCGGACGCTCAAGTACCTGTGGACCGT
  GTGCTGCCACTGCGGGGGCAAGACGAAGGAGGCCCAGAAGATTAACAATGGCTCAAGCCAGGCGGATGGC
  ACTCTCAAACCAGTGGATGAAAAAGAGGAGGCAGTGGCCGCCGAGGTCGGCTGGATGACCTCCGTGAAGG
  ACTGGGCGGGGGTGATGATATCCGCCCAGACACTGACTGGCAGAGTCCTGGTTGTCTTAGTCTTTGCTCT
  CAGCATCGGTGCACTTGTAATATACTTCATAGATTCATCAAACCCAATAGAATCCTGCCAGAATTTCTAC
  AAAGATTTCACATTACAGATCGACATGGCTTTCAACGTGTTCTTCCTTCTCTACTTCGGCTTGCGGTTTA
  TTGCAGCCAACGATAAATTGTGGTTCTGGCTGGAAGTGAACTCTGTAGTGGATTTCTTCACGGTGCCCCC
  CGTGTTTGTGTCTGTGTACTTAAACAGAAGTTGGCTTGGTTTGAGATTTTTAAGAGCTCTGAGACTGATA
  CAGTTTTCAGAAATTTTGCAGTTTCTGAATATTCTTAAAACAAGTAATTCCATCAAGCTGGTGAATCTGC
  TCTCCATATTTATCAGCACGTGGCTGACTGCAGCCGGGTTCATCCATTTGGTGGAGAATTCAGGGGACCC
  ATGGGAAAATTTCCAAAACAACCAGGCTCTCACCTACTGGGAATGTGTCTATTTACTCATGGTCACAATG
  TCCACCGTTGGTTATGGGGATGTTTATGCAAAAACCACACTTGGGCGCCTCTTCATGGTCTTCTTCATCC
  TCGGGGGACTGGCCATGTTTGCCAGCTACGTCCCTGAAATCATAGAGTTAATAGGAAACCGCAAGAAATA
  CGGGGGCTCCTATAGTGCGGTTAGTGGAAGAAAGCACATTGTGGTCTGCGGACACATCACTCTGGAGAGT
  GTTTCCAACTTCCTGAAGGACTTTCTGCACAAGGACCGGGATGACGTCAATGTGGAGATCGTTTTTCTTC
  ACAACATCTCCCCCAACCTGGAGCTTGAAGCTCTGTTCAAACGACATTTTACTCAGGTGGAATTTTATCA
  GGGTTCCGTCCTCAATCCACATGATCTTGCAAGAGTCAAGATAGAGTCAGCAGATGCATGCCTGATCCTT
  GCCAACAAGTACTGCGCTGACCCGGATGCGGAGGATGCCTCGAATATCATGAGAGTAATCTCCATAAAGA
  ACTACCATCCGAAGATAAGAATCATCACTCAAATGCTGCAGTATCACAACAAGGCCCATCTGCTAAACAT
  CCCGAGCTGGAATTGGAAAGAAGGTGATGACGCAATCTGCCTCGCAGAGTTGAAGTTGGGCTTCATAGCC
  CAGAGCTGCCTGGCTCAAGGCCTCTCCACCATGCTTGCCAACCTCTTCTCCATGAGGTCATTCATAAAGA
  TTGAGGAAGACACATGGCAGAAATACTACTTGGAAGGAGTCTCAAATGAAATGTACACAGAATATCTCTC
  CAGTGCCTTCGTGGGTCTGTCCTTCCCTACTGTTTGTGAGCTGTGTTTTGTGAAGCTCAAGCTCCTAATG
  ATAGCCATTGAGTACAAGTCTGCCAACCGAGAGAGCCGTATATTAATTAATCCTGGAAACCATCTTAAGA
  TCCAAGAAGGTACTTTAGGATTTTTCATCGCAAGTGATGCCAAAGAAGTTAAAAGGGCATTTTTTTACTG
  CAAGGCCTGTCATGATGACATCACAGATCCCAAAAGAATAAAAAAATGTGGCTGCAAACGGCCCAAGATG
  TCCATCTACAAGAGAATGAGACGGGCATGTTGTTTTGATTGCGGACGTTCTGAGCGTGACTGCTCATGCA
  TGTCAGGCCGTGTGCGTGGTAACGTGGACACCCTTGAGAGAGCCTTCCCACTTTCTTCTGTCTCTGTTAA
  TGATTGCTCCACCAGTTTCCGTGCCTTTGAAGATGAGCAGCCGTCAACACTATCACCAAAAAAAAAGCAA
  CGGAATGGAGGCATGCGGAACTCACCCAACACCTCGCCTAAGCTGATGAGGCATGACCCCTTGTTAATTC
  CTGGCAATGATCAGATTGACAACATGGACTCCAATGTGAAGAAGTACGACTCTACTGGGATGTTTCACTG
  GTGTGCACCCAAGGAGATAGAGAAAGTCATCCTGACTCGAAGTGAAGCTGCCATGACCGTCCTGAGTGGC
  CATGTCGTGGTCTGCATCTTTGGCGACGTCAGCTCAGCCCTGATCGGCCTCCGGAACCTGGTGATGCCGC
  TCCGTGCCAGCAACTTTCATTACCATGAGCTCAAGCACATTGTGTTTGTGGGCTCTATTGAGTACCTCAA
  GCGGGAATGGGAGACGCTTCATAACTTCCCCAAAGTGTCCATATTGCCTGGTACGCCATTAAGTCGGGCT
  GATTTAAGGGCTGTCAACATCAACCTCTGTGACATGTGCGTTATCCTGTCAGCCAATCAGAATAATATTG
  ATGATACTTCGCTGCAGGACAAGGAATGCATCTTGGCGTCACTCAACATCAAATCTATGCAGTTTGATGA
  CAGCATCGGAGTCTTGCAGGCTAATTCCCAAGGGTTCACACCTCCAGGAATGGATAGATCCTCTCCAGAT
  AACAGCCCAGTGCACGGGATGTTACGTCAACCATCCATCACAACTGGGGTCAACATCCCCATCATCACTG
  AACTAGTGAACGATACTAATGTTCAGTTTTTGGACCAAGACGATGATGATGACCCTGATACAGAACTGTA
  CCTCACGCAGCCCTTTGCCTGTGGGACAGCATTTGCCGTCAGTGTCCTGGACTCACTCATGAGCGCGACG
  TACTTCAATGACAATATCCTCACCCTGATACGGACCCTGGTGACCGGAGGAGCCACGCCGGAGCTGGAGG
  CTCTGATTGCTGAGGAAAACGCCCTTAGAGGTGGCTACAGCACCCCGCAGACACTGGCCAATAGGGACCG
  CTGCCGCGTGGCCCAGTTAGCTCTGCTCGATGGGCCATTTGCGGACTTAGGGGATGGTGGTTGTTATGGT
  GATCTGTTCTGCAAAGCTCTGAAAACATATAATATGCTTTGTTTTGGAATTTACCGGCTGAGAGATGCTC
  ACCTCAGCACCCCCAGTCAGTGCACAAAGAGGTATGTCATCACCAACCCGCCCTATGAGTTTGAGCTCGT
  GCCGACGGACCTGATCTTCTGCTTAATGCAGTTTGACCACAATGCCGGCCAGTCCCGGGCCAGCCTGTCC
  CATTCCTCCCACTCGTCGCAGTCCTCCAGCAAGAAGAGCTCCTCTGTTCACTCCATCCCATCCACAGCAA
  ACCGACAGAACCGGCCCAAGTCCAGGGAGTCCCGGGACAAACAGAAGTACGTGCAGGAAGAGCGGCTT
  
  AGCGGACCGACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG228657 representing NM_001161352
  Red=Cloning site Green=Tags(s)
  
  MANGGGGGGGSSGGGGGGGGSSLRMSSNIHANHLSLDASSSSSSSSSSSSSSSSSSSSSSVHEPKMDALI
  IPVTMEVPCDSRGQRMWWAFLASSMVTFFGGLFIILLWRTLKYLWTVCCHCGGKTKEAQKINNGSSQADG
  TLKPVDEKEEAVAAEVGWMTSVKDWAGVMISAQTLTGRVLVVLVFALSIGALVIYFIDSSNPIESCQNFY
  KDFTLQIDMAFNVFFLLYFGLRFIAANDKLWFWLEVNSVVDFFTVPPVFVSVYLNRSWLGLRFLRALRLI
  QFSEILQFLNILKTSNSIKLVNLLSIFISTWLTAAGFIHLVENSGDPWENFQNNQALTYWECVYLLMVTM
  STVGYGDVYAKTTLGRLFMVFFILGGLAMFASYVPEIIELIGNRKKYGGSYSAVSGRKHIVVCGHITLES
  VSNFLKDFLHKDRDDVNVEIVFLHNISPNLELEALFKRHFTQVEFYQGSVLNPHDLARVKIESADACLIL
  ANKYCADPDAEDASNIMRVISIKNYHPKIRIITQMLQYHNKAHLLNIPSWNWKEGDDAICLAELKLGFIA
  QSCLAQGLSTMLANLFSMRSFIKIEEDTWQKYYLEGVSNEMYTEYLSSAFVGLSFPTVCELCFVKLKLLM
  IAIEYKSANRESRILINPGNHLKIQEGTLGFFIASDAKEVKRAFFYCKACHDDITDPKRIKKCGCKRPKM
  SIYKRMRRACCFDCGRSERDCSCMSGRVRGNVDTLERAFPLSSVSVNDCSTSFRAFEDEQPSTLSPKKKQ
  RNGGMRNSPNTSPKLMRHDPLLIPGNDQIDNMDSNVKKYDSTGMFHWCAPKEIEKVILTRSEAAMTVLSG
  HVVVCIFGDVSSALIGLRNLVMPLRASNFHYHELKHIVFVGSIEYLKREWETLHNFPKVSILPGTPLSRA
  DLRAVNINLCDMCVILSANQNNIDDTSLQDKECILASLNIKSMQFDDSIGVLQANSQGFTPPGMDRSSPD
  NSPVHGMLRQPSITTGVNIPIITELVNDTNVQFLDQDDDDDPDTELYLTQPFACGTAFAVSVLDSLMSAT
  YFNDNILTLIRTLVTGGATPELEALIAEENALRGGYSTPQTLANRDRCRVAQLALLDGPFADLGDGGCYG
  DLFCKALKTYNMLCFGIYRLRDAHLSTPSQCTKRYVITNPPYEFELVPTDLIFCLMQFDHNAGQSRASLS
  HSSHSSQSSSKKSSSVHSIPSTANRQNRPKSRESRDKQKYVQEERL
  
  SGPTRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-RsrII Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001161352
• ORF Size: 3708 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001161352.1, NP_001154824.1
• RefSeq Size: 6294 bp
• RefSeq ORF: 3711 bp
• Locus ID: 3778
• Cytogenetics: 10q22.3
• Protein Families: Druggable Genome, Ion Channels: Potassium, Transmembrane
• Protein Pathways: Vascular smooth muscle contraction
• Gene Summary: 'MaxiK channels are large conductance, voltage and calcium-sensitive potassium channels which are fundamental to the control of smooth muscle tone and neuronal excitability. MaxiK channels can be formed by 2 subunits: the pore-forming alpha subunit, which is the product of this gene, and the modulatory beta subunit. Intracellular calcium regulates the physical association between the alpha and beta subunits. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Jul 2008]'