PHF10 (NM_133325) Human Tagged ORF Clone

CAT#: RG229249

PHF10 (GFP-tagged) - Human PHD finger protein 10 (PHF10), transcript variant 2

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: PHF10
• Synonyms: BAF45A; XAP135
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG229249 representing NM_133325
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGGCGGCGGCCGGGCCCGGGGCTGCGCTGTCCCCGCGGCCGTGCGACAGCGACCCAGCCACCCCCG
  GAGCGCAGTCCCCGAAGGATGATAATGAAGATAATTCAAATGATGGGACCCAGCCATCCAAAAGGAGGCG
  AATGGGCTCAGGAGATAGTTCTAGGAGTTGTGAAACTTCAAGTCAAGATCTTGGTTTTAGTTACTATCCA
  GCAGAAAACTTGATAGAGTACAAATGGCCACCTGATGAAACAGGAGAATACTATATGCTTCAAGAACAAG
  TCAGTGAATATTTGGGTGTGACCTCCTTTAAAAGGAAATATCCAGAGCGACGAGATTTGTCTCACAAGGA
  GAAACTCTACCTGAGAGAGCTAAATGTCATTACTGAAACTCAGTGCACTCTAGGCTTAACAGCATTGCGC
  AGTGATGAAGTGATTGATTTAATGATAAAAGAATATCCAGCCAAACATGCTGAGTATTCTGTTATTCTAC
  AAGAAAAAGAACGTCAACGAATTACAGACCATTATAAAGAGTATTCCCAAATGCAACAACAGAATACTCA
  GAAAGTTGAAGCCAGTAAAGTGCCTGAGTATATTAAGAAAGCTGCCAAAAAAGCAGCAGAATTTAATAGC
  AACTTAAACCGGGAACGCATGGAAGAAAGAAGAGCTTATTTTGACTTGCAGACACATGTTATCCAGGTAC
  CTCAAGGGAAGTACAAAGTTTTGCCAACAGAGCGAACAAAGGTCAGTTCTTACCCAGTGGCTCTCATCCC
  CGGACAGTTCCAGGAATATTATAAGAGGTACTCACCAGATGAGCTGCGGTATCTGCCATTAAACACAGCC
  CTGTATGAGCCCCCTCTGGATCCTGAGCTCCCTGCTCTAGACAGTGATGGTGATTCAGATGATGGCGAAG
  ATGGTCGAGGTGATGAGAAACGGAAAAATAAAGGCACTTCGGACAGCTCCTCTGGCAATGTATCTGAAGG
  GGAAAGCCCTCCTGACAGCCAGGAGGACTCTTTCCAGGGAAGACAGAAATCAAAAGACAAAGCTGCCACT
  CCAAGAAAAGATGGTCCCAAACGTTCTGTACTGTCCAAGTCAGTTCCTGGGTACAAGCCAAAGGTCATTC
  CAAATGCTATATGTGGAATTTGTCTGAAGGGTAAGGAGTCCAACAAGAAAGGAAAGGCTGAATCACTTAT
  ACACTGCTCCCAATGTGAGAATAGTGGCCATCCTTCTTGCCTGGATATGACAATGGAGCTTGTTTCTATG
  ATTAAGACCTACCCATGGCAGTGTATGGAATGTAAAACATGCATTATATGTGGACAACCCCACCATGAAG
  AAGAAATGATGTTCTGTGATATGTGTGACAGAGGTTATCATACTTTTTGTGTGGGCCTTGGTGCTATTCC
  ATCAGGTCGCTGGATTTGTGACTGTTGTCAGCGGGCCCCCCCAACACCCAGGAAAGTGGGCAGAAGGGGG
  AAAAACAGCAAAGAGGGA
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG229249 representing NM_133325
  Red=Cloning site Green=Tags(s)
  
  MAAAAGPGAALSPRPCDSDPATPGAQSPKDDNEDNSNDGTQPSKRRRMGSGDSSRSCETSSQDLGFSYYP
  AENLIEYKWPPDETGEYYMLQEQVSEYLGVTSFKRKYPERRDLSHKEKLYLRELNVITETQCTLGLTALR
  SDEVIDLMIKEYPAKHAEYSVILQEKERQRITDHYKEYSQMQQQNTQKVEASKVPEYIKKAAKKAAEFNS
  NLNRERMEERRAYFDLQTHVIQVPQGKYKVLPTERTKVSSYPVALIPGQFQEYYKRYSPDELRYLPLNTA
  LYEPPLDPELPALDSDGDSDDGEDGRGDEKRKNKGTSDSSSGNVSEGESPPDSQEDSFQGRQKSKDKAAT
  PRKDGPKRSVLSKSVPGYKPKVIPNAICGICLKGKESNKKGKAESLIHCSQCENSGHPSCLDMTMELVSM
  IKTYPWQCMECKTCIICGQPHHEEEMMFCDMCDRGYHTFCVGLGAIPSGRWICDCCQRAPPTPRKVGRRG
  KNSKEG
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_133325
• ORF Size: 1488 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_133325.2, NP_579866.2
• RefSeq Size: 1686
• RefSeq ORF: 1491
• Locus ID: 55274
• Domains: PHD
• Protein Families: Druggable Genome, Stem cell - Pluripotency, Transcription Factors
• Gene Summary: This gene contains a predicted ORF that encodes a protein with two zinc finger domains. The function of the encoded protein is not known. Sequence analysis suggests that multiple alternatively spliced transcript variants are derived from this gene but the full-length nature of only two of them is known. These two splice variants encode different isoforms. A pseudogene for this gene is located on Xq28. [provided by RefSeq, Jul 2008]