ARHGAP25 (NM_001007231) Human Tagged ORF Clone

CAT#: RG229321

ARHGAP25 (GFP-tagged) - Human Rho GTPase activating protein 25 (ARHGAP25), transcript variant 1

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: ARHGAP25
• Synonyms: HEL-S-308; KAIA0053
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG229321 representing NM_001007231
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCCCTAAAATTGCCAAGGAACTGGGATTTCAACCTGAAAGTGGAGGCTGCGAAAATAGCTCGGTCAA
  GGAGTGTGATGACTGGCGAGCAGATGGCTGCCTTCCATCCATCGTCCACCCCCAACCCGCTGGAGAGGCC
  CATCAAGATGGGCTGGCTGAAGAAGCAGAGGTCCATCGTGAAGAACTGGCAGCAGAGGTACTTTGTGCTG
  AGGGCGCAGCAGCTCTACTACTACAAGGATGAAGAGGACACGAAGCCCCAGGGCTGCATGTATCTACCAG
  GATGTACAATCAAGGAGATCGCCACAAACCCAGAAGAAGCTGGGAAGTTTGTCTTTGAAATCATTCCAGC
  CTCATGGGACCAGAATCGCATGGGACAGGACTCCTATGTCCTCATGGCCAGCTCTCAGGCGGAGATGGAG
  GAGTGGGTTAAATTCCTCAGGAGAGTTGCTGGCACACCCTGTGGAGCAGTGTTTGGCCAGCGCTTGGATG
  AGACTGTGGCCTATGAACAGAAATTCGGCCCCCATCTGGTGCCCATCCTGGTGGAGAAATGTGCAGAGTT
  CATCCTGGAGCACGGCCGGAATGAAGAGGGCATCTTCCGTCTGCCTGGGCAGGACAACCTGGTGAAGCAG
  CTGAGAGACGCTTTTGATGCTGGGGAGCGGCCCTCCTTTGACAGAGACACAGATGTGCACACTGTGGCTT
  CCCTGTTAAAGCTCTACCTCCGAGACCTCCCAGAGCCCGTGGTTCCCTGGAGCCAGTACGAAGGGTTCCT
  GCTCTGTGGGCAGCTCACGAATGCGGATGAGGCAAAGGCTCAGCAGGAGTTGATGAAGCAGCTCTCCATC
  CTTCCTCGTGACAACTATAGTCTCCTGAGCTACATCTGCAGGTTCCTACATGAAATACAGCTGAACTGTG
  CTGTTAACAAGATGAGTGTGGACAACCTGGCTACTGTGATTGGTGTGAATCTCATCAGGTCGAAGGTCGA
  AGACCCTGCCGTGATCATGAGAGGGACTCCTCAGATCCAAAGAGTGATGACTATGATGATCAGAGACCAT
  GAAGTCCTCTTCCCCAAGTCCAAGGATATACCCCTGTCACCCCCTGCCCAGAAAAATGACCCCAAGAAAG
  CTCCAGTGGCCCGAAGCTCTGTAGGCTGGGATGCCACTGAAGACCTCCGAATTTCTAGGACAGACAGCTT
  CAGTAGCATGACAAGCGACTCTGATACAACCAGCCCCACCGGACAGCAGCCGAGCGATGCGTTTCCGGAG
  GACAGCAGCAAAGTACCCAGGGAAAAGCCAGGAGACTGGAAAATGCAATCTCGTAAAAGGACTCAAACAC
  TCCCTAACCGGAAATGTTTCTTGACATCAGCTTTTCAGGGTGCCAACAGCAGCAAAATGGAGATCTTTAA
  AAATGAATTCTGGTCGCCTTCCTCAGAGGCTAAGGCAGGGGAAGGGCACAGGAGAACGATGTCTCAAGAC
  TTGCGCCAACTTTCTGACTCCCAACGGACTTCCACCTACGATAACGTCCCTTCCCTGCCAGGGTCCCCTG
  GGGAGGAAGCCAGTGCACTCTCTTCCCAAGCCTGTGACTCCAAGGGAGATACTCTTGCCAGTCCAAACTC
  TGAAACTGGGCCTGGAAAAAAGAACTCTGGAGAAGAGGAAATTGATTCTTTGCAGAGGATGGTCCAAGAG
  CTACGAAAGGAAATAGAAACACAGAAGCAAATGTATGAGGAACAGATTAAAAACCTTGAGAAGGAAAATT
  ATGACGTTTGGGCTAAAGTGGTGAGGCTCAATGAAGAACTGGAGAAGGAAAAGAAGAAGTCTGCAGCCCT
  AGAGATCAGCCTCCGCAACATGGAGCGCTCCCGGGAGGATGTTGAGAAGAGGAACAAGGCCTTGGAAGAA
  GAAGTCAAGGAATTTGTCAAATCCATGAAGGAACCCAAGACCGAGGCT
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG229321 representing NM_001007231
  Red=Cloning site Green=Tags(s)
  
  MSLKLPRNWDFNLKVEAAKIARSRSVMTGEQMAAFHPSSTPNPLERPIKMGWLKKQRSIVKNWQQRYFVL
  RAQQLYYYKDEEDTKPQGCMYLPGCTIKEIATNPEEAGKFVFEIIPASWDQNRMGQDSYVLMASSQAEME
  EWVKFLRRVAGTPCGAVFGQRLDETVAYEQKFGPHLVPILVEKCAEFILEHGRNEEGIFRLPGQDNLVKQ
  LRDAFDAGERPSFDRDTDVHTVASLLKLYLRDLPEPVVPWSQYEGFLLCGQLTNADEAKAQQELMKQLSI
  LPRDNYSLLSYICRFLHEIQLNCAVNKMSVDNLATVIGVNLIRSKVEDPAVIMRGTPQIQRVMTMMIRDH
  EVLFPKSKDIPLSPPAQKNDPKKAPVARSSVGWDATEDLRISRTDSFSSMTSDSDTTSPTGQQPSDAFPE
  DSSKVPREKPGDWKMQSRKRTQTLPNRKCFLTSAFQGANSSKMEIFKNEFWSPSSEAKAGEGHRRTMSQD
  LRQLSDSQRTSTYDNVPSLPGSPGEEASALSSQACDSKGDTLASPNSETGPGKKNSGEEEIDSLQRMVQE
  LRKEIETQKQMYEEQIKNLEKENYDVWAKVVRLNEELEKEKKKSAALEISLRNMERSREDVEKRNKALEE
  EVKEFVKSMKEPKTEA
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001007231
• ORF Size: 1938 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_001007231.2, NP_001007232.2
• RefSeq Size: 3020
• RefSeq ORF: 1941
• Locus ID: 9938
• Gene Summary: ARHGAPs, such as ARHGAP25, encode negative regulators of Rho GTPases (see ARHA; MIM 165390), which are implicated in actin remodeling, cell polarity, and cell migration (Katoh and Katoh, 2004 [PubMed 15254788]). [supplied by OMIM, Mar 2008]