MDFIC (NM_001166346) Human Tagged ORF Clone

CAT#: RG229596

MDFIC (GFP-tagged) - Human MyoD family inhibitor domain containing (MDFIC), transcript variant 2

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: MDFIC
• Synonyms: HIC; MDFIC1
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG229596 representing NM_001166346
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  GTGCGCGGAGTCAGAGCCGCCACCGCTGCCGCAGTTGCCGCCACTGCGGCGTCTGGGCTGAGCCGGAGGG
  AGGCGGGAGGACGCGCAGGGGCGGCCGCCGCCGTCGTCAGGCCACCGGGGCGAAAATGCGGCCGCTGCCG
  GAGGCTCGCTAACTTTCCGGGGCGGAAGAGGAGGAGGAGGAGGAGGAAGGGGCTTGGAGCGACTACGGGG
  GGATGCGGAGAAGCAGTCAGTTCCCTGCACCCAGCACCTCACAGCCCTTCCTCCGTGCGCCCTGCCGGGC
  GGCGAGCTAGGCGGCAGCGGCGCGGCGCGGGCTCGGCGGAGCGGCCCATGTCCGGCGCGGGCGAAGCCCT
  CGCTCCCGGGCCCGTGGGGCCGCAGCGCGTGGCCGAGGCGGGCGGCGGCCAGCTGGGCTCCACAGCCCAG
  GGTCTCTTCAGCAGATCCAGGACTGCCGCAGCTCTC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG229596 representing NM_001166346
  Red=Cloning site Green=Tags(s)
  
  VRGVRAATAAAVAATAASGLSRREAGGRAGAAAAVVRPPGRKCGRCRRLANFPGRKRRRRRRKGLGATTG
  GCGEAVSSLHPAPHSPSSVRPAGRRARRQRRGAGSAERPMSGAGEALAPGPVGPQRVAEAGGGQLGSTAQ
  GLFSRSRTAAAL
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001166346
• ORF Size: 456 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_001166346.1, NP_001159818.3
• RefSeq Size: 891
• RefSeq ORF: 459
• Locus ID: 29969
• Gene Summary: This gene product is a member of a family of proteins characterized by a specific cysteine-rich C-terminal domain, which is involved in transcriptional regulation of viral genome expression. Alternative translation initiation from an upstream non-AUG (GUG), and an in-frame, downstream AUG codon, results in the production of two isoforms, p40 and p32, respectively, which have different subcellular localization; p32 is mainly found in the cytoplasm, whereas p40 is targeted to the nucleolus. Both isoforms have transcriptional regulatory activity that is attributable to the cysteine-rich C-terminal domain. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Oct 2009]