HHAT (NM_001170588) Human Tagged ORF Clone

CAT#: RG230065

HHAT (GFP-tagged) - Human hedgehog acyltransferase (HHAT), transcript variant 4

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: HHAT
• Synonyms: MART2; SKI1; Skn
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG230065 representing NM_001170588
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCTGCCCCGATGGGAACTGGCACTTTACCTACTTGCCTCACTAGGCTTCCACTTCTATTCCTTCTATG
  AAGTTTACAAAGTCTCCAGAGAACACGAAGAGGAGCTGGACCAGGAATTTGAGCTGGAGACTGACACTTT
  ATTTGGAGGATTAAAGAAGGATGCGACCGACTTTGAGTGGAGCTTCTGGATGGAATGGGGGAAGCAGTGG
  CTGGTGTGGCTTCTCCTTGGCCACATGGTAGTGTCTCAAATGGCCACACTGCTGGCAAGAAAGAGAAGGT
  GGTACAAGACAGAAAACGAGTACTACCTGCTGCAGTTCACGCTGACCGTTCGCTGCCTGTACTACACCAG
  CTTCAGCCTGGAGCTCTGCTGGCAGCAGCTGCCTGCTGCATCGACCTCCTACTCCTTTCCCTGGATGCTG
  GCCTATGTCTTTTATTATCCAGTCTTACACAATGGGCCCATCCTCAGCTTCTCGGAGTTCATCAAACAGA
  TGCAGCAGCAGGAGCATGACTCCCTGAAGGCCAGCCTGTGTGTCCTGGCCCTGGGGCTGGGCCGCCTTCT
  TTGCTGGTGGTGGCTGGCCGAGCTGATGGCTCACCTGATGTACATGCATGCCATCTACAGCAGCATCCCC
  CTCCTGGAGACTGTCTCTTGTTGGACCTTAGGAGGACTGGCGTTAGCCCAGGTGCTCTTTTTCTACGTGA
  AGTACTTGGTGCTCTTTGGCGTGCCTGCTCTGCTCATGCGCCTGGATGGACTCACTCCACCCGCCCTCCC
  CCGCTGCGTGAGCACCATGTTCAGTTTCACCGGGATGTGGAGGTATTTTGATGTTGGACTGCATAATTTC
  TTAATCAGGTATGTGTACATTCCAGTGGGCGGGTCCCAGCATGGCCTGCTGGGGACACTGTTTTCCACGG
  CGATGACATTTGCATTTGTGAGCTACTGGCATGGCGGCTACGACTACCTCTGGTGCTGGGCAGCGCTCAA
  CTGGCTGGGAGTCACTGTGGAGAATGGAGTCCGGAGGCTGGTGGAGACTCCCTGCATCCAGGACAGTCTG
  GCCCGATACTTCTCCCCACAAGCTCGCCGTCGATTCCACGCTGCCCTTGCTTCTTGTTCCACCTCGATGC
  TGATCCTGTCCAACCTGGTATTTCTTGGGGGCAATGAGGTTGGGAAAACCTACTGGAATAGGATCTTCAT
  ACAAGGCTGGCCTTGGGTGACCCTCTCTGTCCTGGGATTCCTGTACTGCTACTCCCACGTGGGCATTGCC
  TGGGCCCAGACCTACGCCACGGAC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG230065 representing NM_001170588
  Red=Cloning site Green=Tags(s)
  
  MLPRWELALYLLASLGFHFYSFYEVYKVSREHEEELDQEFELETDTLFGGLKKDATDFEWSFWMEWGKQW
  LVWLLLGHMVVSQMATLLARKRRWYKTENEYYLLQFTLTVRCLYYTSFSLELCWQQLPAASTSYSFPWML
  AYVFYYPVLHNGPILSFSEFIKQMQQQEHDSLKASLCVLALGLGRLLCWWWLAELMAHLMYMHAIYSSIP
  LLETVSCWTLGGLALAQVLFFYVKYLVLFGVPALLMRLDGLTPPALPRCVSTMFSFTGMWRYFDVGLHNF
  LIRYVYIPVGGSQHGLLGTLFSTAMTFAFVSYWHGGYDYLWCWAALNWLGVTVENGVRRLVETPCIQDSL
  ARYFSPQARRRFHAALASCSTSMLILSNLVFLGGNEVGKTYWNRIFIQGWPWVTLSVLGFLYCYSHVGIA
  WAQTYATD
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001170588
• ORF Size: 1284 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_001170588.1, NP_001164059.1
• RefSeq Size: 3446
• RefSeq ORF: 1287
• Locus ID: 55733
• Protein Families: Transmembrane
• Gene Summary: 'Skinny hedgehog' (SKI1) encodes an enzyme that acts within the secretory pathway to catalyze amino-terminal palmitoylation of 'hedgehog' (see MIM 600725). [supplied by OMIM, Jul 2002]