PPP2R2A (NM_001177591) Human Tagged ORF Clone

CAT#: RG230117

PPP2R2A (GFP-tagged) - Human protein phosphatase 2, regulatory subunit B, alpha (PPP2R2A), transcript variant 2

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: PPP2R2A
• Synonyms: B55A; B55ALPHA; PR52A; PR55A; PR55alpha
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG230117 representing NM_001177591
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTTCCCGAAGTTTTCTCTTCGTTCTATGTTTCATGGAGCTGGAGGAGGGAATGATATTCAGTGGTGTT
  TTTCTCAGGTGAAAGGAGCAGTAGATGATGATGTAGCAGAAGCAGATATAATTTCTACAGTAGAATTTAA
  TCATTCTGGAGAATTACTAGCAACAGGAGATAAAGGTGGTAGAGTTGTCATCTTTCAACAGGAGCAGGAG
  AACAAAATCCAGTCTCATAGCAGAGGAGAATATAATGTTTACAGCACCTTCCAGAGCCATGAACCAGAGT
  TTGACTACTTGAAAAGTTTAGAAATAGAAGAAAAGATCAACAAAATTAGGTGGTTACCCCAGAAAAATGC
  TGCTCAGTTTTTATTGTCTACCAATGATAAAACAATAAAATTATGGAAAATCAGTGAAAGGGACAAAAGA
  CCAGAAGGGTATAACTTGAAAGAGGAGGATGGAAGGTATAGAGATCCTACTACAGTTACTACACTACGAG
  TGCCAGTCTTTAGGCCTATGGATCTAATGGTTGAGGCCAGTCCACGAAGAATATTTGCCAATGCTCATAC
  ATATCACATCAACTCAATTTCTATTAATAGTGATTATGAAACATATTTATCTGCAGATGATTTGCGGATT
  AATCTTTGGCATCTGGAAATTACAGACAGGAGTTTTAACATTGTGGATATCAAGCCTGCCAATATGGAAG
  AGCTAACAGAGGTGATTACAGCAGCAGAATTTCATCCAAACAGCTGTAACACATTTGTATACAGCAGCAG
  TAAAGGAACTATTCGGCTATGTGACATGAGGGCATCTGCCCTCTGTGATAGACATTCTAAATTGTTTGAA
  GAACCTGAAGATCCCAGTAACAGGTCATTTTTTTCCGAAATCATCTCCTCTATTTCGGATGTAAAATTCA
  GCCATAGTGGTCGATATATGATGACTAGAGACTATTTGTCAGTCAAAATTTGGGACTTAAATATGGAAAA
  CAGGCCTGTGGAAACATACCAGGTGCATGAATACCTCAGAAGTAAACTCTGTTCACTGTATGAAAATGAC
  TGCATATTTGACAAATTTGAATGTTGTTGGAATGGATCTGACAGTGTTGTCATGACTGGATCTTACAATA
  ATTTCTTCAGAATGTTTGACAGAAACACAAAGCGAGACATAACCCTAGAAGCATCGCGGGAAAACAATAA
  GCCTCGCACAGTTCTGAAGCCTCGCAAAGTCTGTGCAAGTGGCAAGCGAAAGAAAGATGAAATAAGTGTT
  GACAGCCTAGACTTCAATAAGAAAATCCTTCACACAGCCTGGCACCCCAAGGAAAATATCATTGCCGTAG
  CTACTACAAACAATCTGTATATATTTCAAGACAAAGTGAAT
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG230117 representing NM_001177591
  Red=Cloning site Green=Tags(s)
  
  MFPKFSLRSMFHGAGGGNDIQWCFSQVKGAVDDDVAEADIISTVEFNHSGELLATGDKGGRVVIFQQEQE
  NKIQSHSRGEYNVYSTFQSHEPEFDYLKSLEIEEKINKIRWLPQKNAAQFLLSTNDKTIKLWKISERDKR
  PEGYNLKEEDGRYRDPTTVTTLRVPVFRPMDLMVEASPRRIFANAHTYHINSISINSDYETYLSADDLRI
  NLWHLEITDRSFNIVDIKPANMEELTEVITAAEFHPNSCNTFVYSSSKGTIRLCDMRASALCDRHSKLFE
  EPEDPSNRSFFSEIISSISDVKFSHSGRYMMTRDYLSVKIWDLNMENRPVETYQVHEYLRSKLCSLYEND
  CIFDKFECCWNGSDSVVMTGSYNNFFRMFDRNTKRDITLEASRENNKPRTVLKPRKVCASGKRKKDEISV
  DSLDFNKKILHTAWHPKENIIAVATTNNLYIFQDKVN
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001177591
• ORF Size: 1371 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001177591.1, NP_001171062.1
• RefSeq Size: 3677 bp
• RefSeq ORF: 1374 bp
• Locus ID: 5520
• Cytogenetics: 8p21.2
• Protein Families: Druggable Genome, Phosphatase
• Protein Pathways: Tight junction
• Gene Summary: 'The product of this gene belongs to the phosphatase 2 regulatory subunit B family. Protein phosphatase 2 is one of the four major Ser/Thr phosphatases, and it is implicated in the negative control of cell growth and division. It consists of a common heteromeric core enzyme, which is composed of a catalytic subunit and a constant regulatory subunit, that associates with a variety of regulatory subunits. The B regulatory subunit might modulate substrate selectivity and catalytic activity. This gene encodes an alpha isoform of the regulatory subunit B55 subfamily. Alternatively spliced transcript variants have been described. [provided by RefSeq, Apr 2010]'