TCTN1 (NM_001173976) Human Tagged ORF Clone

CAT#: RG230156

TCTN1 (GFP-tagged) - Human tectonic family member 1 (TCTN1), transcript variant 5

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: TCTN1
• Synonyms: JBTS13; TECT1
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG230156 representing NM_001173976
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGATCACAGCTCACTGCAGCCTCGACCTCCTAGGCTCAGTTGCTGTTCTCTGTGTCTGTGACTTATCCC
  CAGCACAGTGTGACATCAACTGCTGCTGTGATCCCGACTGCAGCTCCGTGGATTTCAGTGTCTTTTCTGC
  CTGCTCAGTTCCAGTTGTCACGGGCGACAGCCAGTTTTGTAGTCAAAAAGCAGTCATCTATTCATTGAAT
  TTTACAGCAAACCCACCTCAAAGAGTATTTGAACTTGTTGACCAGATTAATCCATCTATTTTCTGCATTC
  ATATTACAAACTATAAACCTGCATTATCCTTTATTAATCCAGAAGTACCTGATGAAAACAATTTTGATAC
  ATTGATGAAAACATCTGATGGTTTTACATTGAATGCTGAATCATATGTTTCCTTCACAACCAAACTGGAT
  ATTCCTACTGCTGCTAAATATGAGTATGGGGTTCCTCTGCAGACTTCAGATTCGTTTCTGAGATTTCCTT
  CGTCCCTGACATCATCTCTGTGCACTGATAATAACCCTGCAGCGTTTCTGGTGAACCAGGCTGTTAAGTG
  CACCAGAAAAATAAATTTAGAACAGTGTGAAGAAATTGAAGCCCTCAGCATGGCTTTTTACAGCAGCCCG
  GAAATTCTGAGGGTACCTGATTCAAGAAAAAAGGTCCCTATCACTGTTCAGTCCATCGTCATTCAGTCTC
  TAAATAAAACGCTCACCCGACGGGAGGACACTGATGTGCTGCAGCCGACTCTCGTCAACGCTGGACACTT
  TAGCCTTTGCGTGAATGTTGTTCTTGAGGTAAAGTACAGCCTCACATACACAGATGCAGGTGAAGTCACC
  AAAGCTGATCTCTCATTCGTTCTGGGGACAGTTAGCAGCGTAGTGGTCCCACTGCAGCAAAAGTTTGAAA
  TTCATTTTCTTCAGGAAAATACCCAGCCAGTCCCTCTCAGTGGAAACCCTGGTTATGTCGTGGGGCTCCC
  ATTAGCTGCTGGATTCCAGCCTCATAAGGGTGGAGCTCTCCCGTGTCAGCTCGTAGCACAGAAGGTGAAG
  AGCCTGCTGTGGGGCCAGGGCTTCCCAGATTACGTGGCCCCTTTTGGAAATTCCCAGGCCCAGGACATGC
  TGGACTGGGTGCCCATCCACTTCATCACCCAGTCATTCAACAGGAAGCATTTTGTTTTGCAGGATTCCTG
  CCAGCTCCCAGGGGCTTTGGTTATAGAAGTGAAGTGGACTAAATACGGATCCCTGCTGAATCCACAGGCC
  AAAATAGTCAATGTAACTGCAAATCTAATTTCATCCTCCTTTCCTGAGGCCAACTCAGGAAATGAAAGGA
  CGATTCTTATTTCCACTGCGGTTACTTTTGTGGATGTGTCTGCACCTGCAGAGGCAGGCTTCAGAGCTCC
  ACCAGCCATCAATGCCAGGCTGCCCTTTAACTTCTTCTTCCCGTTTGTT
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG230156 representing NM_001173976
  Red=Cloning site Green=Tags(s)
  
  MITAHCSLDLLGSVAVLCVCDLSPAQCDINCCCDPDCSSVDFSVFSACSVPVVTGDSQFCSQKAVIYSLN
  FTANPPQRVFELVDQINPSIFCIHITNYKPALSFINPEVPDENNFDTLMKTSDGFTLNAESYVSFTTKLD
  IPTAAKYEYGVPLQTSDSFLRFPSSLTSSLCTDNNPAAFLVNQAVKCTRKINLEQCEEIEALSMAFYSSP
  EILRVPDSRKKVPITVQSIVIQSLNKTLTRREDTDVLQPTLVNAGHFSLCVNVVLEVKYSLTYTDAGEVT
  KADLSFVLGTVSSVVVPLQQKFEIHFLQENTQPVPLSGNPGYVVGLPLAAGFQPHKGGALPCQLVAQKVK
  SLLWGQGFPDYVAPFGNSQAQDMLDWVPIHFITQSFNRKHFVLQDSCQLPGALVIEVKWTKYGSLLNPQA
  KIVNVTANLISSSFPEANSGNERTILISTAVTFVDVSAPAEAGFRAPPAINARLPFNFFFPFV
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001173976
• ORF Size: 1449 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_001173976.1, NP_001167447.1
• RefSeq Size: 1969
• RefSeq ORF: 1452
• Locus ID: 79600
• Protein Families: Secreted Protein
• Gene Summary: This gene encodes a member of a family of secreted and transmembrane proteins. The orthologous gene in mouse functions downstream of smoothened and rab23 to modulate hedgehog signal transduction. This protein is a component of the tectonic-like complex, which forms a barrier between the ciliary axoneme and the basal body. A mutation in this gene was found in a family with Joubert syndrome-13. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Feb 2016]