FUS (NM_001170937) Human Tagged ORF Clone

CAT#: RG230222

FUS (GFP-tagged) - Human fused in sarcoma (FUS), transcript variant 4. Note: ORF is codon optimized

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: FUS
• Synonyms: ALS6; ETM4; FUS1; HNRNPP2; POMP75; TLS
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG230222 ORF sequence, codon optimized.
  Due to the complexity of NM_001170937, the ORF clone is codon optimized for mammalian Expression.
  The nucleotide sequence differs from the reference sequence, yet the amino acid sequence remains identical.
  
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCAGCAATGACTATACGCAGCAGGCCACCCAATCCTACGGCGCCTACCCAACTCAGCCCGGTCAGG
  GTTACTCCCAGCAGTCCTCCCAACCATACGGACAGCAGAGCTATAGTGGATATAGCCAAAGCACGGATAC
  ATCTGGGTATGGCCAGTCATCATACAGCAGTTACGGGCAATCACAGAACACCGGCTACGGAACGCAGAGT
  ACCCCACAGGGTTACGGATCTACAGGCGGCTATGGCTCTAGCCAGTCATCCCAAAGTAGCTATGGGCAGC
  AGAGCTCTTACCCGGGGTACGGGCAGCAGCCTGCCCCGAGTTCAACCAGCGGATCCTACGGCTCTTCCAG
  CCAGTCTTCTAGCTACGGCCAGCCCCAGTCCGGCTCCTACTCTCAGCAGCCTTCCTATGGCGGTCAGCAG
  CAGTCATACGGCCAGCAGCAAAGTTATAACCCACCACAAGGTTACGGACAGCAGAACCAGTATAATTCTT
  CCTCAGGGGGAGGGGGCGGCGGGAATTACGGACAGGACCAGAGCTCCATGTCTAGCGGGGGGGGCTCTGG
  CGGCGGATATGGCAATCAGGATCAGTCCGGGGGCGGAGGGAGCGGTGGATATGGCCAGCAGGACAGGGGC
  GGCCGCGGGAGAGGCGGGAGCGGAGGAGGGGGAGGCGGAGGGGGCGGGGGATACAACCGGAGCAGTGGCG
  GTTATGAGCCTCGCGGTCGAGGGGGAGGGAGAGGGGGCAGAGGCGGTATGGGTGGAAGCGACCGCGGGGG
  TTTTAACAAGTTTGGAGGGCCACGGGACCAAGGCAGCAGACATGATTCCGAGCAAGATAACTCCGACAAC
  AACACCATTTTCGTCCAAGGCCTTGGAGAGAACGTCACAATCGAAAGCGTGGCCGATTACTTTAAGCAGA
  TTGGAATAATCAAAACAAACAAAAAGACCGGCCAACCTATGATTAACTTGTACACCGACCGCGAAACAGG
  CAAGCTTAAGGGAGAGGCAACCGTCAGTTTTGATGATCCACCCAGCGCCAAAGCGGCCATTGATTGGTTC
  GATGGCAAGGAGTTCTCTGGCAATCCCATTAAGGTAAGCTTTGCAACGAGGCGCGCTGATTTCAATAGAG
  GCGGGGGAAATGGTCGCGGAGGGCGCGGACGAGGAGGACCAATGGGGCGGGGGGGTTACGGCGGGGGGGG
  GAGCGGTGGCGGTGGGAGGGGGGGGTTCCCTAGCGGGGGAGGGGGTGGTGGGGGTCAGCAGAGGGCTGGG
  GACTGGAAATGTCCCAACCCCACTTGTGAGAATATGAACTTCAGCTGGCGAAATGAATGCAACCAGTGCA
  AGGCGCCGAAACCTGATGGACCTGGTGGGGGACCAGGGGGCTCACACATGGGCGGCAATTACGGGGATGA
  CCGAAGGGGCGGGCGCGGCGGCTATGATAGAGGCGGCTACCGCGGGCGGGGAGGTGACAGGGGTGGGTTC
  CGCGGGGGGAGGGGTGGCGGTGATAGGGGAGGCTTCGGTCCTGGCAAGATGGATTCCAGAGGCGAACACA
  GGCAGGACAGGAGGGAGAGGCCCTAC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG230222 representing NM_001170937
  Red=Cloning site Green=Tags(s)
  
  MASNDYTQQATQSYGAYPTQPGQGYSQQSSQPYGQQSYSGYSQSTDTSGYGQSSYSSYGQSQNTGYGTQS
  TPQGYGSTGGYGSSQSSQSSYGQQSSYPGYGQQPAPSSTSGSYGSSSQSSSYGQPQSGSYSQQPSYGGQQ
  QSYGQQQSYNPPQGYGQQNQYNSSSGGGGGGNYGQDQSSMSSGGGSGGGYGNQDQSGGGGSGGYGQQDRG
  GRGRGGSGGGGGGGGGGYNRSSGGYEPRGRGGGRGGRGGMGGSDRGGFNKFGGPRDQGSRHDSEQDNSDN
  NTIFVQGLGENVTIESVADYFKQIGIIKTNKKTGQPMINLYTDRETGKLKGEATVSFDDPPSAKAAIDWF
  DGKEFSGNPIKVSFATRRADFNRGGGNGRGGRGRGGPMGRGGYGGGGSGGGGRGGFPSGGGGGGGQQRAG
  DWKCPNPTCENMNFSWRNECNQCKAPKPDGPGGGPGGSHMGGNYGDDRRGGRGGYDRGGYRGRGGDRGGF
  RGGRGGGDRGGFGPGKMDSRGEHRQDRRERPY
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001170937
• ORF Size: 1566 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001170937.1, NP_001164408.1
• RefSeq Size: 5107 bp
• RefSeq ORF: 1569 bp
• Locus ID: 2521
• Cytogenetics: 16p11.2
• Protein Families: Druggable Genome, Stem cell - Pluripotency
• Gene Summary: 'This gene encodes a multifunctional protein component of the heterogeneous nuclear ribonucleoprotein (hnRNP) complex. The hnRNP complex is involved in pre-mRNA splicing and the export of fully processed mRNA to the cytoplasm. This protein belongs to the FET family of RNA-binding proteins which have been implicated in cellular processes that include regulation of gene expression, maintenance of genomic integrity and mRNA/microRNA processing. Alternative splicing results in multiple transcript variants. Defects in this gene result in amyotrophic lateral sclerosis type 6. [provided by RefSeq, Sep 2009]'