CARD8 (NM_001184900) Human Tagged ORF Clone

CAT#: RG230245

CARD8 (GFP-tagged) - Human caspase recruitment domain family, member 8 (CARD8), transcript variant 1

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: CARD8
• Synonyms: CARDINAL; DACAR; DAKAR; NDPP; NDPP1; TUCAN
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG230245 representing NM_001184900
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAAAAAAAGGAGTGTCCAGAAAAGAGTAGCAGCAGTGAGGAAGAGCTGCCGAGACGGGACAGTGGAT
  CCAGTAGGAACATAGATGCATCCAAACTCATTAGACTACAAGGATCACGGAAACTGTTGGTTGACAATAG
  CATACGGGAACTGCAATACACAAAAACTGGAATTTTTTTTCAGGCTGAGGCCTGTGTGACAAATGATACG
  GTATACAGGGAGCTACCCTGTGTTTCTGAGACCCTTTGTGACATCTCACATTTTTTCCAAGAAGATGATG
  AGACAGAGGCAGAGCCATTATTGTTCCGTGCTGTTCCTGAGTGTCAACTATCTGGGGGGGACATTCCCAG
  TGTATCAGAAGAGCAGGAATCTTCAGAGGGACAAGATTCAGGAGACATTTGCTCAGAAGAGAATCAAATA
  GTTTCCTCTTATGCTTCTAAAGTCTGTTTTGAGATCGAAGAAGATTATAAAAATCGTCAGTTTCTGGGGC
  CTGAAGGAAATGTGGATGTTGAGTTGATTGATAAGAGCACAAACAGATACAGCGTTTGGTTCCCCACTGC
  TGGCTGGTATCTGTGGTCAGCCACAGGCCTCGGCTTCCTGGTAAGGGATGAGGTCACAGTGACGATTGCG
  TTTGGTTCCTGGAGTCAGCACCTGGCCCTGGACCTGCAGCACCATGAACAGTGGCTGGTGGGCGGCCCCT
  TGTTTGATGTCACTGCAGAGCCAGAGGAGGCTGTCGCCGAAATCCACCTCCCCCACTTCATCTCCCTCCA
  AGCAGGTGAGGTGGACGTCTCCTGGTTTCTCGTTGCCCATTTTAAGAATGAAGGGATGGTCCTGGAGCAT
  CCAGCCCGGGTGGAGCCTTTCTATGCTGTCCTGGAAAGCCCCAGCTTCTCTCTGATGGGCATCCTGCTGC
  GGATCGCCAGTGGGACTCGCCTCTCCATCCCCATCACTTCCAACACATTGATCTATTATCACCCCCACCC
  CGAAGATATTAAGTTCCACTTGTACCTTGTCCCCAGCGACGCCTTGCTAACAAAGGCGATAGATGATGAG
  GAAGATCGCTTCCATGGTGTGCGCCTGCAGACTTCGCCCCCAATGGAACCCCTGAACTTTGGTTCCAGTT
  ATATTGTGTCTAATTCTGCTAACCTGAAAGTAATGCCCAAGGAGTTGAAATTGTCCTACAGGAGCCCTGG
  AGAAATTCAGCACTTCTCAAAATTCTATGCTGGGCAGATGAAGGAACCCATTCAACTTGAGATTACTGAA
  AAAAGACATGGGACTTTGGTGTGGGATACTGAGGTGAAGCCAGTGGATCTCCAGCTTGTAGCTGCATCAG
  CCCCTCCTCCTTTCTCAGGTGCAGCCTTTGTGAAGGAGAACCACCGGCAACTCCAAGCCAGGATGGGGGA
  CCTGAAAGGGGTGCTCGATGATCTCCAAGACAATGAGGTTCTTACTGAGAATGAGAAGGAGCTGGTGGAG
  CAGGAAAAGACACGGCAGAGCAAGAATGAGGCCTTGCTGAGCATGGTGGAGAAGAAAGGGGACCTGGCCC
  TGGACGTGCTCTTCAGAAGCATTAGTGAAAGGGACCCTTACCTCGTGTCCTATCTTAGACAGCAGAATTT
  G
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG230245 representing NM_001184900
  Red=Cloning site Green=Tags(s)
  
  MEKKECPEKSSSSEEELPRRDSGSSRNIDASKLIRLQGSRKLLVDNSIRELQYTKTGIFFQAEACVTNDT
  VYRELPCVSETLCDISHFFQEDDETEAEPLLFRAVPECQLSGGDIPSVSEEQESSEGQDSGDICSEENQI
  VSSYASKVCFEIEEDYKNRQFLGPEGNVDVELIDKSTNRYSVWFPTAGWYLWSATGLGFLVRDEVTVTIA
  FGSWSQHLALDLQHHEQWLVGGPLFDVTAEPEEAVAEIHLPHFISLQAGEVDVSWFLVAHFKNEGMVLEH
  PARVEPFYAVLESPSFSLMGILLRIASGTRLSIPITSNTLIYYHPHPEDIKFHLYLVPSDALLTKAIDDE
  EDRFHGVRLQTSPPMEPLNFGSSYIVSNSANLKVMPKELKLSYRSPGEIQHFSKFYAGQMKEPIQLEITE
  KRHGTLVWDTEVKPVDLQLVAASAPPPFSGAAFVKENHRQLQARMGDLKGVLDDLQDNEVLTENEKELVE
  QEKTRQSKNEALLSMVEKKGDLALDVLFRSISERDPYLVSYLRQQNL
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001184900
• ORF Size: 1611 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_001184900.1, NP_001171829.1
• RefSeq Size: 5291
• RefSeq ORF: 1614
• Locus ID: 22900
• Protein Families: Druggable Genome
• Protein Pathways: NOD-like receptor signaling pathway
• Gene Summary: The protein encoded by this gene belongs to the caspase recruitment domain (CARD)-containing family of proteins, which are involved in pathways leading to activation of caspases or nuclear factor kappa-B (NFKB). This protein may be a component of the inflammasome, a protein complex that plays a role in the activation of proinflammatory caspases. It is thought that this protein acts as an adaptor molecule that negatively regulates NFKB activation, CASP1-dependent IL1B secretion, and apoptosis. Polymorphisms in this gene may be associated with a susceptibility to rheumatoid arthritis. Alternatively spliced transcript variants have been described for this gene. [provided by RefSeq, May 2010]