KLHL13 (NM_001168301) Human Tagged ORF Clone

CAT#: RG230380

KLHL13 (GFP-tagged) - Human kelch-like 13 (Drosophila) (KLHL13), transcript variant 4

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: KLHL13
• Synonyms: BKLHD2
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG230380 representing NM_001168301
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGATCATCTGCATAGAGGGGAATTGGTTGCTGCAATACTTCGTAACCGATCTCTCGTGGAAGAGGAAG
  ACCAACACATGAAATTGTCCCTTGGAGGCAGCGAAATGGGCCTCTCATCCCATTTGCAGTCTTCCAAGGC
  AGGACCTACACGCATCTTTACCAGCAATACCCACAGTTCTGTGGTGTTACAGGGCTTTGACCAGCTTCGA
  CTTGAAGGATTGCTTTGTGATGTGACCCTGATGCCAGGTGACACAGATGATGCTTTCCCTGTGCATAGAG
  TCATGATGGCATCTGCTAGTGATTACTTCAAGGCTATGTTCACAGGTGGAATGAAAGAACAAGATTTAAT
  GTGCATTAAACTTCATGGTGTGAGCAAAGTCGGTCTAAGGAAAATTATTGATTTCATTTATACTGCAAAG
  CTTTCTCTTAATATGGACAACCTTCAAGACACGCTGGAAGCTGCCAGTTTCCTACAGATTCTGCCAGTTT
  TGGACTTCTGTAAAGTGTTTCTCATATCTGGGGTCACTTTAGACAACTGTGTTGAAGTTGGACGGATTGC
  CAACACCTACAATCTAACCGAAGTGGATAAATACGTTAACAGTTTCGTCTTGAAGAATTTTCCTGCATTG
  CTGAGCACAGGGGAGTTCTTGAAACTCCCTTTTGAGCGTCTTGCCTTCGTGCTTTCCAGTAATAGCCTTA
  AGCACTGTACTGAACTTGAGCTCTTTAAGGCTACCTGTCGTTGGCTTCGCCTGGAAGAGCCTCGGATGGA
  CTTTGCTGCAAAATTAATGAAGAACATACGATTTCCACTGATGACACCACAGGAGCTCATTAATTACGTG
  CAAACGGTGGATTTCATGAGAACTGACAATACTTGTGTGAATTTGCTTTTGGAAGCCAGCAATTACCAAA
  TGATGCCATATATGCAGCCAGTTATGCAGTCAGACAGGACTGCCATTAGGTCTGACACCACTCACTTGGT
  TACACTAGGAGGAGTGCTGAGGCAGCAGCTGGTTGTCAGTAAGGAATTGCGCATGTATGATGAAAAGGCC
  CATGAGTGGAAATCGTTAGCCCCCATGGATGCCCCAAGGTACCAGCATGGCATCGCCGTCATTGGAAATT
  TTCTCTATGTGGTTGGCGGACAGAGTAATTATGATACAAAAGGAAAAACGGCAGTTGATACAGTCTTCAG
  ATTTGATCCTCGATACAATAAATGGATGCAAGTTGCATCTTTAAATGAAAAGCGCACCTTCTTCCACCTA
  AGTGCCCTCAAAGGATATCTGTATGCAGTTGGTGGGCGAAATGCAGCAGGTGAACTGCCCACAGTAGAAT
  GTTACAATCCAAGAACAAATGAATGGACCTATGTTGCCAAAATGAGTGAGCCCCACTATGGCCATGCTGG
  AACTGTGTATGGAGGAGTGATGTATATTTCAGGAGGAATTACTCATGATACTTTCCAAAAGGAGCTCATG
  TGCTTTGACCCTGATACTGACAAATGGATCCAGAAGGCGCCAATGACCACTGTCAGAGGTCTGCATTGCA
  TGTGTACAGTGGGAGAAAGGCTCTATGTCATTGGTGGCAATCACTTCAGAGGAACAAGTGATTATGATGA
  TGTCCTAAGCTGTGAATACTATTCACCTATCCTTGACCAGTGGACCCCAATTGCTGCCATGTTAAGAGGG
  CAGAGTGATGTTGGGGTCGCTGTCTTCGAAAATAAAATCTATGTGGTTGGGGGGTATTCTTGGAATAATC
  GTTGTATGGTAGAGATAGTGCAGAAATATGATCCAGATAAAGATGAATGGCATAAGGTTTTTGATCTGCC
  AGAATCCCTTGGTGGCATTCGTGCTTGCACACTCACAGTTTTTCCACCAGAAGAAACCACACCATCACCT
  TCTAGAGAGTCCCCTCTTTCTGCACCT
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG230380 representing NM_001168301
  Red=Cloning site Green=Tags(s)
  
  MDHLHRGELVAAILRNRSLVEEEDQHMKLSLGGSEMGLSSHLQSSKAGPTRIFTSNTHSSVVLQGFDQLR
  LEGLLCDVTLMPGDTDDAFPVHRVMMASASDYFKAMFTGGMKEQDLMCIKLHGVSKVGLRKIIDFIYTAK
  LSLNMDNLQDTLEAASFLQILPVLDFCKVFLISGVTLDNCVEVGRIANTYNLTEVDKYVNSFVLKNFPAL
  LSTGEFLKLPFERLAFVLSSNSLKHCTELELFKATCRWLRLEEPRMDFAAKLMKNIRFPLMTPQELINYV
  QTVDFMRTDNTCVNLLLEASNYQMMPYMQPVMQSDRTAIRSDTTHLVTLGGVLRQQLVVSKELRMYDEKA
  HEWKSLAPMDAPRYQHGIAVIGNFLYVVGGQSNYDTKGKTAVDTVFRFDPRYNKWMQVASLNEKRTFFHL
  SALKGYLYAVGGRNAAGELPTVECYNPRTNEWTYVAKMSEPHYGHAGTVYGGVMYISGGITHDTFQKELM
  CFDPDTDKWIQKAPMTTVRGLHCMCTVGERLYVIGGNHFRGTSDYDDVLSCEYYSPILDQWTPIAAMLRG
  QSDVGVAVFENKIYVVGGYSWNNRCMVEIVQKYDPDKDEWHKVFDLPESLGGIRACTLTVFPPEETTPSP
  SRESPLSAP
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001168301
• ORF Size: 1917 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_001168301.1, NP_001161773.1
• RefSeq Size: 3121
• RefSeq ORF: 1920
• Locus ID: 90293
• Protein Pathways: Ubiquitin mediated proteolysis
• Gene Summary: This gene encodes a BTB and kelch domain containing protein and belongs to the kelch repeat domain containing superfamily of proteins. The encoded protein functions as an adaptor protein that complexes with Cullin 3 and other proteins to form the Cullin 3-based E3 ubiquitin-protein ligase complex. This complex is necessary for proper chromosome segregation and completion of cytokinesis. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Mar 2010]