MUSK (NM_001166280) Human Tagged ORF Clone

CAT#: RG230497

MUSK (GFP-tagged) - Human muscle, skeletal, receptor tyrosine kinase (MUSK), transcript variant 2

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: MUSK
• Synonyms: CMS9; FADS; FADS1
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG230497 representing NM_001166280
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGAGAGCTCGTCAACATTCCACTGGTACATATTCTTACTCTGGTTGCCTTCAGCGGAACTGAGAAAC
  TTCCAAAAGCTCCTGTCATCACCACTCCTCTTGAAACAGTGGATGCCTTAGTTGAAGAAGTGGCTACTTT
  CATGTGTGCAGTGGAATCCTACCCCCAGCCTGAGATTTCCTGGACTAGAAATAAAATTCTCATTAAACTC
  TTTGACACCCGGTACAGCATCCGGGAGAATGGGCAGCTCCTCACCATCCTGAGTGTGGAAGACAGTGATG
  ATGGCATTTACTGCTGCACGGCCAACAATGGTGTGGGAGGAGCTGTGGAGAGTTGTGGAGCCCTGCAAGT
  GAAGATGAAACCTAAAATAACTCGTCCTCCCATAAATGTGAAAATAATAGAGGGATTAAAAGCAGTCCTA
  CCATGTACTACAATGGGTAATCCCAAACCATCAGTGTCTTGGATAAAGGGAGACAGCCCTCTCAGGGAAA
  ATTCCCGAATTGCAGTTCTTGAATCTGGGAGCTTGAGGATTCATAACGTACAAAAGGAAGATGCAGGACA
  GTATCGATGTGTGGCAAAAAACAGCCTCGGGACAGCATATTCCAAAGTGGTGAAGCTGGAAGTTGAGGAA
  GAAAGTGAACCCGAACAAGATACTAAAGTTTTTGCCAGGATCCTGCGGGCTCCTGAATCCCACAATGTCA
  CCTTTGGCTCCTTTGTGACCCTGCACTGTACAGCAACAGGCATTCCTGTCCCCACCATCACCTGGATTGA
  AAACGGAAATGCTGTTTCTTCTGGGTCCATTCAAGAGAGTGTGAAAGACCGAGTGATTGACTCAAGACTG
  CAGCTGTTTATCACCAAGCCAGGACTCTACACATGCATAGCTACCAATAAGCATGGGGAGAAGTTCAGTA
  CTGCCAAGGCTGCAGCCACCATCAGCATAGCAGAATGGAGAGAGTACTGCTTGGCAGTAAAGGAGCTCTT
  CTGCGCAAAAGAATGGCTGGTAATGGAAGAGAAGACCCACAGAGGACTCTACAGATCCGAGATGCATTTG
  CTGTCCGTGCCAGAATGCAGCAAGCTTCCCAGCATGCATTGGGACCCCACGGCCTGTGCCAGACTGCCAC
  ATCTAGCATTCCCACCAATGACGTCCTCAAAGCCAAGTGTGGACATTCCAAATCTGCCTTCCTCCTCCTC
  TTCTTCCTTCTCTGTCTCACCTACATACTCCATGACTGTAATAATCTCCATCATGTCCAGCTTTGCAATA
  TTTGTGCTTCTTACCATAACTACTCTCTATTGCTGCCGAAGAAGAAAACAATGGAAAAATAAGAAAAGAG
  AATCAGCAGCAGTAACCCTCACCACACTGCCTTCTGAGCTCTTACTAGATAGACTTCATCCCAACCCCAT
  GTACCAGAGGATGCCGCTCCTTCTGAACCCCAAATTGCTCAGCCTGGAGTATCCAAGGAATAACATTGAA
  TATGTGAGAGACATCGGAGAGGGAGCGTTTGGAAGGGTGTTTCAAGCAAGGGCACCAGGCTTACTTCCCT
  ATGAACCTTTCACTATGGTGGCAGTAAAGATGCTCAAAGAAGAAGCCTCGGCAGATATGCAAGCGGACTT
  TCAGAGGGAGGCAGCCCTCATGGCAGAATTTGACAACCCTAACATTGTGAAGCTATTAGGAGTGTGTGCT
  GTCGGGAAGCCAATGTGCCTGCTCTTTGAATACATGGCCTATGGTGACCTCAATGAGTTCCTCCGCAGCA
  TGTCCCCTCACACCGTGTGCAGCCTCAGTCACAGTGACTTGTCTATGAGGGCTCAGGTCTCCAGCCCTGG
  GCCCCCACCCCTCTCCTGTGCTGAGCAGCTTTGCATTGCCAGGCAGGTGGCAGCTGGCATGGCTTACCTC
  TCAGAACGTAAGTTTGTTCACCGAGATTTAGCCACCAGGAACTGCCTGGTGGGCGAGAACATGGTGGTGA
  AAATTGCCGACTTTGGCCTCTCCAGGAACATCTACTCAGCAGACTACTACAAAGCTAATGAAAACGACGC
  TATCCCTATCCGTTGGATGCCACCAGAGTCCATTTTTTATAACCGCTACACTACAGAGTCTGATGTGTGG
  GCCTATGGCGTGGTCCTCTGGGAGATCTTCTCCTATGGCCTGCAGCCCTACTATGGGATGGCCCATGAGG
  AGGTCATTTACTACGTGCGAGATGGCAACATCCTCTCCTGCCCTGAGAACTGCCCCGTGGAGCTGTACAA
  TCTCATGCGTCTATGTTGGAGCAAGCTGCCTGCAGACAGACCCAGTTTCACCAGTATTCACCGAATTCTG
  GAACGCATGTGTGAGAGGGCAGAGGGAACTGTGAGTGTC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG230497 representing NM_001166280
  Red=Cloning site Green=Tags(s)
  
  MRELVNIPLVHILTLVAFSGTEKLPKAPVITTPLETVDALVEEVATFMCAVESYPQPEISWTRNKILIKL
  FDTRYSIRENGQLLTILSVEDSDDGIYCCTANNGVGGAVESCGALQVKMKPKITRPPINVKIIEGLKAVL
  PCTTMGNPKPSVSWIKGDSPLRENSRIAVLESGSLRIHNVQKEDAGQYRCVAKNSLGTAYSKVVKLEVEE
  ESEPEQDTKVFARILRAPESHNVTFGSFVTLHCTATGIPVPTITWIENGNAVSSGSIQESVKDRVIDSRL
  QLFITKPGLYTCIATNKHGEKFSTAKAAATISIAEWREYCLAVKELFCAKEWLVMEEKTHRGLYRSEMHL
  LSVPECSKLPSMHWDPTACARLPHLAFPPMTSSKPSVDIPNLPSSSSSSFSVSPTYSMTVIISIMSSFAI
  FVLLTITTLYCCRRRKQWKNKKRESAAVTLTTLPSELLLDRLHPNPMYQRMPLLLNPKLLSLEYPRNNIE
  YVRDIGEGAFGRVFQARAPGLLPYEPFTMVAVKMLKEEASADMQADFQREAALMAEFDNPNIVKLLGVCA
  VGKPMCLLFEYMAYGDLNEFLRSMSPHTVCSLSHSDLSMRAQVSSPGPPPLSCAEQLCIARQVAAGMAYL
  SERKFVHRDLATRNCLVGENMVVKIADFGLSRNIYSADYYKANENDAIPIRWMPPESIFYNRYTTESDVW
  AYGVVLWEIFSYGLQPYYGMAHEEVIYYVRDGNILSCPENCPVELYNLMRLCWSKLPADRPSFTSIHRIL
  ERMCERAEGTVSV
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001166280
• ORF Size: 2349 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001166280.1, NP_001159752.1
• RefSeq Size: 2496 bp
• RefSeq ORF: 2352 bp
• Locus ID: 4593
• Cytogenetics: 9q31.3
• Protein Families: Druggable Genome, Protein Kinase, Transmembrane
• Gene Summary: 'This gene encodes a muscle-specific tyrosine kinase receptor. The encoded protein may play a role in clustering of the acetylcholine receptor in the postsynaptic neuromuscular junction. Mutations in this gene have been associated with congenital myasthenic syndrome. Alternatively spliced transcript variants have been described.[provided by RefSeq, Oct 2009]'