MUSK (NM_001166280) Human Tagged ORF Clone

CAT#: RC600069

MUSK (DDK-His-tagged)-Extra Cellular Domain Clone of Homo sapiens muscle, skeletal, receptor tyrosine kinase, transcript variant 2, Signal peptide (1-23) plus EC domain (24-409)

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: DDK-His
• Symbol: MUSK
• Synonyms: CMS9; FADS; FADS1
• Vector: pCMV6-XL5-DDK-His
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  ORF Nucleotide Sequence

  >RC600069 representing leader sequence plus the extracellular domain region of NM_001166280
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GTAATACGACTCACTATAGGGCGGCCGCGAATTCGTCGACTGGATCTGGTACCGAGGAGATCCGCCGCCG
  CGATCGCC
  
  ATGAGAGAGCTCGTCAACATTCCACTGGTACATATTCTTACTCTGGTTGCCTTCAGCGGAACTGAGAAAC
  TTCCAAAAGCTCCTGTCATCACCACTCCTCTTGAAACAGTGGATGCCTTAGTTGAAGAAGTGGCTACTTT
  CATGTGTGCAGTGGAATCCTACCCCCAGCCTGAGATTTCCTGGACTAGAAATAAAATTCTCATTAAACTC
  TTTGACACCCGGTACAGCATCCGGGAGAATGGGCAGCTCCTCACCATCCTGAGTGTGGAAGACAGTGATG
  ATGGCATTTACTGCTGCACGGCCAACAATGGTGTGGGAGGAGCTGTGGAGAGTTGTGGAGCCCTGCAAGT
  GAAGATGAAACCTAAAATAACTCGTCCTCCCATAAATGTGAAAATAATAGAGGGATTAAAAGCAGTCCTA
  CCATGTACTACAATGGGTAATCCCAAACCATCAGTGTCTTGGATAAAGGGAGACAGCCCTCTCAGGGAAA
  ATTCCCGAATTGCAGTTCTTGAATCTGGGAGCTTGAGGATTCATAACGTACAAAAGGAAGATGCAGGACA
  GTATCGATGTGTGGCAAAAAACAGCCTCGGGACAGCATATTCCAAAGTGGTGAAGCTGGAAGTTGAGGAA
  GAAAGTGAACCCGAACAAGATACTAAAGTTTTTGCCAGGATCCTGCGGGCTCCTGAATCCCACAATGTCA
  CCTTTGGCTCCTTTGTGACCCTGCACTGTACAGCAACAGGCATTCCTGTCCCCACCATCACCTGGATTGA
  AAACGGAAATGCTGTTTCTTCTGGGTCCATTCAAGAGAGTGTGAAAGACCGAGTGATTGACTCAAGACTG
  CAGCTGTTTATCACCAAGCCAGGACTCTACACATGCATAGCTACCAATAAGCATGGGGAGAAGTTCAGTA
  CTGCCAAGGCTGCAGCCACCATCAGCATAGCAGAATGGAGAGAGTACTGCTTGGCAGTAAAGGAGCTCTT
  CTGCGCAAAAGAATGGCTGGTAATGGAAGAGAAGACCCACAGAGGACTCTACAGATCCGAGATGCATTTG
  CTGTCCGTGCCAGAATGCAGCAAGCTTCCCAGCATGCATTGGGACCCCACGGCCTGTGCCAGACTGCCAC
  ATCTAGCATTCCCACCAATGACGTCCTCAAAGCCAAGTGTGGACATTCCAAATCTGCCTTCCTCCTCCTC
  TTCTTCCTTCTCTGTCTCACCTACATACTCCATGACT
  
  ACGCGTTCAGGCGACTACAAGGATGACGACGATAAGGGATCTCATCATCACCATCACCATTAATGAGATC
  TGGTACCGATATCAAGCTTGTCGACTCTAGA
  Protein Sequence

  >RC600069 representing signal peptide plus the extracellular domain region of NM_001166280
  Red=Cloning sites Green= DDK and 6XHIS Tags
  
  MRELVNIPLVHILTLVAFSGTEKLPKAPVITTPLETVDALVEEVATFMCAVESYPQPEISWTRNKILIKL
  FDTRYSIRENGQLLTILSVEDSDDGIYCCTANNGVGGAVESCGALQVKMKPKITRPPINVKIIEGLKAVL
  PCTTMGNPKPSVSWIKGDSPLRENSRIAVLESGSLRIHNVQKEDAGQYRCVAKNSLGTAYSKVVKLEVEE
  ESEPEQDTKVFARILRAPESHNVTFGSFVTLHCTATGIPVPTITWIENGNAVSSGSIQESVKDRVIDSRL
  QLFITKPGLYTCIATNKHGEKFSTAKAAATISIAEWREYCLAVKELFCAKEWLVMEEKTHRGLYRSEMHL
  LSVPECSKLPSMHWDPTACARLPHLAFPPMTSSKPSVDIPNLPSSSSSSFSVSPTYSMT
  
  TRSGTRSGDYKDDDDKGSHHHHHH
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NM_001166280
• ORF Size: 1227 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the extra cellular domain of the protein with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001166280.1, NP_001159752.1
• RefSeq Size: 2496 bp
• RefSeq ORF: 2352 bp
• Locus ID: 4593
• Cytogenetics: 9q31.3
• Protein Families: Druggable Genome, Protein Kinase, Transmembrane
• MW: 45 kDa
• Gene Summary: 'This gene encodes a muscle-specific tyrosine kinase receptor. The encoded protein may play a role in clustering of the acetylcholine receptor in the postsynaptic neuromuscular junction. Mutations in this gene have been associated with congenital myasthenic syndrome. Alternatively spliced transcript variants have been described.[provided by RefSeq, Oct 2009]'