GPRASP2 (NM_001184874) Human Tagged ORF Clone

CAT#: RG230528

GPRASP2 (GFP-tagged) - Human G protein-coupled receptor associated sorting protein 2 (GPRASP2), transcript variant 3

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: GPRASP2
• Synonyms: DFNX7; GASP2
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG230528 representing NM_001184874
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGACTGGGGCAGAGATTGAGCCTAGTGCCCAGGCCAAGCCTGAAAAGAAGGCTGGGGAAGAGGTTATCG
  CTGGGCCTGAGAGAGAGAATGATGTCCCTCTGGTGGTCAGACCCAAGGTTAGGACCCAGGCAACTACTGG
  GGCAAGGCCCAAAACTGAGACCAAGTCTGTGCCTGCGGCAAGGCCCAAAACTGAGGCCCAAGCAATGTCT
  GGGGCAAGGCCCAAAACTGAGGTCCAAGTAATGGGTGGTGCAAGACCCAAAACGGAGGCTCAAGGAATCA
  CAGGGGCCAGGCCCAAAACCGATGCCAGGGCAGTAGGTGGCGCTCGTTCTAAAACTGATGCCAAGGCAAT
  CCCTGGAGCAAGGCCCAAGGATGAGGCCCAGGCATGGGCCCAGAGTGAATTTGGGACTGAAGCAGTGTCA
  CAGGCAGAAGGAGTGTCCCAGACTAATGCCGTTGCTTGGCCACTGGCCACTGCTGAGTCTGGATCAGTTA
  CTAAATCTAAGGGCCTGTCTATGGATAGAGAACTAGTCAATGTGGATGCTGAAACCTTTCCTGGCACCCA
  GGGTCAGAAAGGAATCCAGCCCTGGTTTGGACCAGGGGAGGAGACTAATATGGGGTCTTGGTGCTATTCC
  AGGCCCAGGGCCAGAGAGGAGGCCTCTAATGAGTCTGGGTTCTGGTCAGCAGATGAGACCTCTACAGCGT
  CTTCTTTCTGGACTGGAGAAGAGACAAGTGTCAGATCATGGCCCAGGGAAGAGTCCAATACCAGGTCCAG
  GCACAGGGCTAAACATCAGACTAATCCCAGGTCCAGGCCCAGATCCAAGCAAGAAGCCTATGTTGATTCC
  TGGTCTGGATCTGAGGATGAGGCCAGCAACCCATTCTCCTTCTGGGTTGGAGAAAATACCAATAACTTGT
  TCAGGCCCAGAGTCAGGGAGGAGGCAAATATCAGGTCCAAGCTCAGGACAAATAGAGAAGATTGTTTTGA
  ATCTGAGTCTGAAGATGAGTTCTATAAGCAGTCCTGGGTTTTGCCTGGAGAAGAGGCCAATAGTAGATTC
  AGGCACAGAGACAAAGAAGATCCTAATACTGCCTTGAAACTCAGGGCCCAGAAAGATGTTGACAGTGATA
  GGGTCAAACAAGAACCCAGGTTTGAGGAGGAAGTCATTATTGGGTCCTGGTTCTGGGCAGAAAAAGAGGC
  CAGTTTGGAGGGTGGAGCTTCAGCAATCTGTGAATCTGAGCCAGGAACTGAGGAGGGGGCCATTGGCGGA
  TCCGCGTACTGGGCTGAGGAAAAGTCCAGTTTGGGGGCTGTGGCCAGAGAAGAGGCCAAGCCGGAGTCTG
  AAGAAGAGGCCATATTTGGGTCCTGGTTCTGGGACAGAGATGAGGCCTGCTTTGACCTAAATCCCTGTCC
  TGTGTACAAGGTCAGTGATAGGTTCAGAGATGCAGCTGAGGAGCTTAATGCATCCTCCAGGCCCCAAACC
  TGGGACGAGGTCACTGTTGAATTCAAACCTGGTCTTTTTCATGGGGTTGGCTTCCGATCCACAAGCCCCT
  TTGGAATTCCCGAAGAGGCTTCTGAAATGCTTGAGGCAAAGCCCAAGAACCTGGAACTTAGCCCAGAAGG
  AGAAGAGCAGGAATCTTTGCTTCAGCCTGATCAGCCTAGTCCTGAGTTCACATTTCAGTATGATCCTTCC
  TACCGGTCAGTCCGGGAAATTCGAGAGCATCTTAGGGCCAGGGAGAGTGCAGAGTCTGAGAGTTGGTCCT
  GCAGCTGCATACAATGTGAGCTGAAAATTGGTTCTGAAGAGTTTGAAGAATTCCTTTTATTAATGGACAA
  AATTCGGGATCCTTTTATTCATGAAATATCTAAAATTGCAATGGGTATGAGAAGTGCTTCTCAATTTACC
  CGAGATTTCATTCGAGATTCAGGTGTTGTCTCACTTATTGAAACCTTGCTTAATTATCCATCCTCTAGAG
  TTAGGACAAGTTTTTTGGAAAATATGATTCACATGGCTCCACCTTATCCAAATCTAAACATGATTGAGAC
  ATTCATATGTCAAGTGTGTGAGGAAACCCTTGCACATAGTGTGGATTCCCTTGAGCAGCTGACTGGAATA
  AGGATGCTTAGACACCTCACTATGACTATTGACTATCACACACTGATTGCCAACTATATGTCCGGGTTTC
  TCTCCTTATTAACCACAGCCAATGCGAGAACGAAGTTTCACGTTCTGAAAATGCTATTGAATTTGTCTGA
  AAATCCTGCTGTGGCAAAAAAACTATTCAGTGCCAAAGCTCTTTCAATATTTGTGGGTCTCTTTAACATA
  GAAGAGACAAATGATAATATTCAAATTGTTATTAAAATGTTTCAGAATATCAGTAACATTATAAAAAGTG
  GAAAGATGTCCTTAATTGATGATGATTTCAGTCTTGAGCCGCTTATTTCTGCATTTCGTGAATTTGAGGA
  GTTAGCTAAGCAACTACAAGCCCAAATAGACAACCAAAATGATCCTGAGGTGGGACAACAAAGT
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG230528 representing NM_001184874
  Red=Cloning site Green=Tags(s)
  
  MTGAEIEPSAQAKPEKKAGEEVIAGPERENDVPLVVRPKVRTQATTGARPKTETKSVPAARPKTEAQAMS
  GARPKTEVQVMGGARPKTEAQGITGARPKTDARAVGGARSKTDAKAIPGARPKDEAQAWAQSEFGTEAVS
  QAEGVSQTNAVAWPLATAESGSVTKSKGLSMDRELVNVDAETFPGTQGQKGIQPWFGPGEETNMGSWCYS
  RPRAREEASNESGFWSADETSTASSFWTGEETSVRSWPREESNTRSRHRAKHQTNPRSRPRSKQEAYVDS
  WSGSEDEASNPFSFWVGENTNNLFRPRVREEANIRSKLRTNREDCFESESEDEFYKQSWVLPGEEANSRF
  RHRDKEDPNTALKLRAQKDVDSDRVKQEPRFEEEVIIGSWFWAEKEASLEGGASAICESEPGTEEGAIGG
  SAYWAEEKSSLGAVAREEAKPESEEEAIFGSWFWDRDEACFDLNPCPVYKVSDRFRDAAEELNASSRPQT
  WDEVTVEFKPGLFHGVGFRSTSPFGIPEEASEMLEAKPKNLELSPEGEEQESLLQPDQPSPEFTFQYDPS
  YRSVREIREHLRARESAESESWSCSCIQCELKIGSEEFEEFLLLMDKIRDPFIHEISKIAMGMRSASQFT
  RDFIRDSGVVSLIETLLNYPSSRVRTSFLENMIHMAPPYPNLNMIETFICQVCEETLAHSVDSLEQLTGI
  RMLRHLTMTIDYHTLIANYMSGFLSLLTTANARTKFHVLKMLLNLSENPAVAKKLFSAKALSIFVGLFNI
  EETNDNIQIVIKMFQNISNIIKSGKMSLIDDDFSLEPLISAFREFEELAKQLQAQIDNQNDPEVGQQS
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001184874
• ORF Size: 2514 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_001184874.2, NP_001171803.1
• RefSeq Size: 3946
• RefSeq ORF: 2517
• Locus ID: 114928
• Protein Families: Druggable Genome
• Gene Summary: The protein encoded by this gene is a member of a family that regulates the activity of G protein-coupled receptors (GPCRs). The encoded protein has been shown to be capable of interacting with several GPCRs, including the M1 muscarinic acetylcholine receptor and the calcitonin receptor. Several transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, May 2010]