BCAR1 (NM_001170719) Human Tagged ORF Clone

CAT#: RG230550

BCAR1 (GFP-tagged) - Human breast cancer anti-estrogen resistance 1 (BCAR1), transcript variant 7

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: BCAR1
• Synonyms: CAS; CAS1; CASS1; CRKAS; P130Cas
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG230550 representing NM_001170719
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGAACGTGCTGGCCAAAGCGCTCTATGACAATGTGGCCGAGTCCCCGGATGAGCTCTCCTTCCGCA
  AGGGTGACATCATGACGGTGCTGGAGCAGGACACGCAGGGCCTGGACGGCTGGTGGCTCTGCTCGCTGCA
  TGGGCGCCAGGGCATCGTGCCTGGGAACCGCCTCAAGATCTTGGTGGGCATGTATGATAAGAAGCCAGCA
  GGGCCTGGCCCCGGCCCTCCCGCCACCCCGGCCCAGCCTCAGCCTGGCCTCCATGCCCCAGCGCCTCCGG
  CCTCCCAGTACACGCCCATGCTCCCCAACACCTACCAGCCCCAGCCAGACAGCGTCTACCTGGTGCCCAC
  TCCCAGCAAGGCTCAGCAAGGCCTCTACCAAGTCCCGGGTCCCAGCCCTCAGTTCCAGTCTCCCCCAGCC
  AAGCAGACATCCACCTTCTCGAAGCAGACACCCCATCACCCGTTTCCCAGCCCGGCCACAGACCTGTACC
  AGGTGCCCCCAGGGCCTGGAGGCCCTGCCCAGGATATTTACCAGGTGCCACCTTCTGCCGGGATGGGGCA
  TGACATCTACCAGGTCCCCCCGTCCATGGACACACGCAGCTGGGAGGGCACGAAGCCCCCGGCAAAGGTG
  GTGGTGCCCACCCGCGTGGGGCAGGGCTATGTATACGAGGCCGCCCAGCCGGAGCAGGACGAGTACGACA
  TCCCGCGACACCTGCTGGCCCCGGGGCCACAGGACATCTATGATGTGCCCCCGGTTCGGGGGCTGCTTCC
  CAGCCAGTATGGCCAGGAGGTGTATGACACACCCCCCATGGCTGTCAAGGGTCCCAATGGCCGAGACCCG
  TTGCTGGAGGTGTATGACGTGCCCCCCAGTGTGGAGAAGGGCCTGCCACCGTCCAACCACCACGCAGTCT
  ACGACGTTCCTCCATCGGTGAGCAAGGATGTGCCCGATGGCCCACTGCTGCGTGAGGAGACCTACGATGT
  GCCCCCCGCCTTCGCCAAGGCCAAGCCCTTTGACCCGGCCCGCACCCCACTGGTACTGGCTGCGCCCCCT
  CCAGACTCCCCGCCGGCCGAGGACGTGTATGACGTGCCGCCCCCGGCTCCTGACCTCTACGACGTGCCCC
  CTGGCTTGCGGCGGCCTGGCCCGGGCACCCTGTACGATGTGCCCCGTGAACGGGTGCTTCCTCCTGAGGT
  GGCTGATGGTGGCGTGGTCGACAGTGGTGTGTATGCGGTGCCTCCCCCAGCTGAACGTGAAGCCCCGGCA
  GAGGGCAAGCGCCTGTCGGCCTCCAGCACCGGCAGCACACGCAGCAGCCAGTCTGCGTCCTCCTTGGAGG
  TGGCAGGGCCGGGCCGGGAACCCCTGGAGCTGGAAGTTGCTGTGGAGGCCCTGGCACGGCTGCAGCAGGG
  TGTGAGCGCCACCGTTGCCCACCTTCTGGACCTGGCAGGCAGCGCCGGTGCGACTGGGAGCTGGCGTAGC
  CCCTCTGAGCCACAGGAGCCGCTGGTGCAGGACCTGCAGGCTGCTGTGGCCGCTGTCCAGAGTGCCGTCC
  ACGAGCTGTTGGAGTTTGCCCGCAGCGCGGTGGGCAATGCTGCCCACACATCTGACCGTGCCCTGCATGC
  CAAGCTTAGCCGGCAGCTGCAGAAGATGGAGGACGTGCACCAGACGCTGGTGGCACATGGTCAGGCCCTC
  GACGCTGGCCGGGGAGGCTCTGGAGCCACCCTTGAGGACCTGGACCGGCTGGTGGCCTGCTCGCGGGCTG
  TGCCCGAGGACGCCAAGCAGCTGGCCTCCTTCCTGCACGGCAATGCCTCACTGCTCTTCAGACGGACCAA
  GGCCACTGCCCCGGGGCCTGAGGGGGGTGGCACCCTGCACCCCAACCCCACTGACAAGACCAGCAGCATC
  CAGTCACGACCCCTGCCCTCACCCCCTAAGTTCACCTCCCAGGACTCGCCAGATGGGCAGTACGAGAACA
  GCGAGGGGGGCTGGATGGAGGACTATGACTACGTCCACCTACAGGGGAAGGAGGAGTTTGAGAAGACCCA
  GAAGGAGCTGCTGGAAAAGGGCAGCATCACGCGGCAGGGCAAGAGCCAGCTGGAGTTGCAGCAGCTGAAG
  CAGTTTGAACGACTGGAACAGGAGGTGTCACGGCCCATAGACCACGACCTGGCCAACTGGACGCCAGCCC
  AACCCCTGGCCCCGGGGCGAACAGGCGGCCTGGGGCCCTCGGACCGGCAGCTGCTGCTCTTCTACCTGGA
  GCAGTGTGAGGCCAACCTGACCACACTGACCAACGCCGTGGACGCCTTCTTTACCGCCGTGGCCACCAAC
  CAGCCGCCCAAGATCTTTGTGGCGCACAGCAAGTTCGTCATCCTCAGCGCCCACAAGCTGGTGTTCATCG
  GGGACACACTGTCACGGCAGGCCAAGGCTGCTGACGTGCGCAGCCAGGTGACCCACTACAGCAACCTGCT
  GTGCGACCTCCTGCGCGGCATCGTGGCCACCACCAAGGCCGCTGCCTTGCAGTACCCATCGCCTTCCGCG
  GCCCAGGACATGGTGGAGAGGGTCAAGGAGCTGGGCCACAGCACCCAGCAGTTCCGCCGCGTCCTAGGCC
  AGCTGGCAGCCGCC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG230550 representing NM_001170719
  Red=Cloning site Green=Tags(s)
  
  MENVLAKALYDNVAESPDELSFRKGDIMTVLEQDTQGLDGWWLCSLHGRQGIVPGNRLKILVGMYDKKPA
  GPGPGPPATPAQPQPGLHAPAPPASQYTPMLPNTYQPQPDSVYLVPTPSKAQQGLYQVPGPSPQFQSPPA
  KQTSTFSKQTPHHPFPSPATDLYQVPPGPGGPAQDIYQVPPSAGMGHDIYQVPPSMDTRSWEGTKPPAKV
  VVPTRVGQGYVYEAAQPEQDEYDIPRHLLAPGPQDIYDVPPVRGLLPSQYGQEVYDTPPMAVKGPNGRDP
  LLEVYDVPPSVEKGLPPSNHHAVYDVPPSVSKDVPDGPLLREETYDVPPAFAKAKPFDPARTPLVLAAPP
  PDSPPAEDVYDVPPPAPDLYDVPPGLRRPGPGTLYDVPRERVLPPEVADGGVVDSGVYAVPPPAEREAPA
  EGKRLSASSTGSTRSSQSASSLEVAGPGREPLELEVAVEALARLQQGVSATVAHLLDLAGSAGATGSWRS
  PSEPQEPLVQDLQAAVAAVQSAVHELLEFARSAVGNAAHTSDRALHAKLSRQLQKMEDVHQTLVAHGQAL
  DAGRGGSGATLEDLDRLVACSRAVPEDAKQLASFLHGNASLLFRRTKATAPGPEGGGTLHPNPTDKTSSI
  QSRPLPSPPKFTSQDSPDGQYENSEGGWMEDYDYVHLQGKEEFEKTQKELLEKGSITRQGKSQLELQQLK
  QFERLEQEVSRPIDHDLANWTPAQPLAPGRTGGLGPSDRQLLLFYLEQCEANLTTLTNAVDAFFTAVATN
  QPPKIFVAHSKFVILSAHKLVFIGDTLSRQAKAADVRSQVTHYSNLLCDLLRGIVATTKAAALQYPSPSA
  AQDMVERVKELGHSTQQFRRVLGQLAAA
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001170719
• ORF Size: 2604 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_001170719.1, NP_001164190.1
• RefSeq Size: 3372
• RefSeq ORF: 2607
• Locus ID: 9564
• Protein Families: Druggable Genome
• Protein Pathways: Chemokine signaling pathway, Focal adhesion, Leukocyte transendothelial migration, Regulation of actin cytoskeleton
• Gene Summary: The protein encoded by this gene is a member of the Crk-associated substrate (CAS) family of scaffold proteins, characterized by the presence of multiple protein-protein interaction domains and many serine and tyrosine phosphorylation sites. The encoded protein contains a Src-homology 3 (SH3) domain, a proline-rich domain, a substrate domain which contains 15 repeat of the YxxP consensus phosphorylation motif for Src family kinases, a serine-rich domain, and a bipartite Src-binding domain, which can bind both SH2 and SH3 domains. This adaptor protein functions in multiple cellular pathways, including in cell motility, apoptosis and cell cycle control. Dysregulation of this gene can have a wide range of effects, affecting different pathways, including cardiac development, vascular smooth muscle cells, liver and kidney function, endothelial migration, and cancer. [provided by RefSeq, Sep 2017]