VARS2 (NM_001167733) Human Tagged ORF Clone

CAT#: RG230585

VARS2 (GFP-tagged) - Human valyl-tRNA synthetase 2, mitochondrial (putative) (VARS2), transcript variant 3

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: VARS2
• Synonyms: COXPD20; VALRS; VARS2L; VARSL
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG230585 representing NM_001167733
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTGTATCCCACCTCCCAATGTCACTGGCTCCCTGCACATTGGCCACGCACTCACGGTGGCCATACAGG
  ATGCCCTCGTGCGCTGGCACCGGATGCGTGGGGATCAAGTGCTGTGGGTCCCTGGTTCAGATCATGCAGG
  AATTGCTACACAAGCTGTGGTGGAGAAACAACTGTGGAAGGAACGGGGAGTGAGGAGACATGAGCTGAGC
  CGGGAGGCCTTCCTTAGGGAGGTGTGGCAGTGGAAGGAGGCGAAAGGTGGAGAGATCTGTGAGCAGCTGC
  GAGCTCTGGGTGCCTCCCTGGACTGGGATCGAGAGTGTTTTACCATGGATGTTGGCTCCTCAGTGGCTGT
  GACTGAAGCTTTTGTGCGGCTCTACAAGGCGGGGTTGCTGTACCGGAACCATCAGCTTGTCAACTGGTCA
  TGTGCTTTAAGATCAGCCATCTCGGACATTGAGGTGGAGAACCGGCCCCTGCCTGGCCACACACAGCTTC
  GACTGCCTGGCTGCCCCACCCCCGTGTCTTTTGGCCTCCTATTTTCTGTTGCCTTCCCCGTGGATGGAGA
  GCCTGATGCAGAGGTTGTGGTAGGAACCACAAGGCCAGAGACGCTGCCTGGAGATGTGGCTGTGGCCGTT
  CATCCAGACGACTCGCGATACACACATCTACACGGGCGACAGCTTCGTCACCCCTTGATGGGGCAGCCTC
  TTCCCCTCATCACAGACTATGCTGTTCAGCCACATGTGGGCACGGGGGCAGTGAAGGTGACTCCAGCTCA
  CAGTCCTGCCGATGCTGAGATGGGGGCCCGACATGGCTTGAGCCCCTTGAATGTCATTGCGGAGGATGGG
  ACCATGACCTCCCTCTGCGGGGACTGGCTGCAGGGTCTTCACCGGTTTGTGGCCCGGGAAAAGATAATGT
  CTGTGCTGAGTGAATGGGGCCTGTTCCGGGGCCTCCAGAACCACCCCATGGTACTGCCCATCTGCAGCCG
  TTCTGGGGATGTGATAGAATACCTGCTGAAGAACCAGTGGTTTGTCCGCTGCCAGGAAATGGGGGCCCGA
  GCTGCCAAGGCTGTGGAGTCGGGGGCCCTGGAGCTCAGTCCCTCCTTCCACCAGAAGAACTGGCAGCACT
  GGTTTTCCCATATTGGGGACTGGTGTGTCTCCCGGCAGCTGTGGTGGGGCCATCAGATTCCAGCCTACCT
  GGTTGTAGAGGACCATGCGCAGGGAGAAGAGGACTGTTGGGTGGTTGGGCGGTCAGAGGCTGAGGCCAGA
  GAGGTAGCAGCGGAACTGACAGGGAGGCCAGGGGCAGAGCTGACCCTGGAGAGGGATCCTGATGTCCTAG
  ACACATGGTTTTCTTCTGCCCTGTTCCCCTTTTCTGCCCTGGGCTGGCCCCAAGAGACCCCAGACCTTGC
  TCGTTTCTACCCCCTGTCACTTTTGGAAACGGGCAGCGACCTTCTGCTGTTCTGGGTGGGCCGCATGGTC
  ATGTTGGGGACCCAGCTCACAGGGCAGCTGCCCTTCAGCAAGGTGCTTCTTCATCCCATGGTTCGGGACA
  GGCAGGGCCGGAAGATGAGCAAGTCCCTGGGGAATGTGCTGGACCCAAGAGACATCATCAGTGGGGTGGA
  GATGCAGGTGCTGCAGGAAAAGCTGAGAAGCGGAAATTTGGACCCTGCAGAGCTGGCCATTGTGGCTGCA
  GCACAGAAAAAGGACTTTCCTCACGGGATCCCTGAGTGTGGGACAGATGCCCTGAGATTCACACTCTGCT
  CCCATGGAGTTCAGGCGGGCGACTTGCACCTGTCAGTCTCTGAGGTCCAGAGCTGCCGACATTTCTGCAA
  CAAGATCTGGAATGCTCTTCGCTTTATCCTCAATGCTTTAGGGGAGAAATTTGTGCCACAGCCTGCTGAG
  GAGCTGTCTCCCTCCTCCCCGATGGATGCCTGGATCCTGAGCCGCCTTGCCCTGGCTGCCCAGGAGTGTG
  AGCGGGGCTTCCTCACCCGAGAGCTCTCGCTCGTCACTCATGCCCTGCACCACTTCTGGCTTCACAACCT
  CTGTGACGTCTACCTGGAGGCTGTGAAGCCCGTGCTGTGGCACTCGCCCCGCCCCCTGGGGCCCCCTCAG
  GTCCTGTTCTCCTGCGCTGACCTCGGCCTCCGCCTCCTGGCCCCACTGATGCCCTTCCTGGCTGAAGAGC
  TCTGGCAGAGGCTGCCCCCCAGGCCTGGTTGCCCCCCTGCCCCCAGCATCTCGGTTGCCCCCTACCCCAG
  CGCCTGCAGCTTGGAGCACTGGCGCCAGCCAGAGCTGGAGCGGCGCTTCTCCCGGGTCCAAGAGGTCGTG
  CAGGTGCTAAGGGCTCTCCGAGCCACGTACCAGCTCACCAAAGCCCGGCCCCGAGTGCTGCTGCAGAGCT
  CAGAGCCTGGGGACCAGGGCCTCTTCGAGGCCTTCTTGGAGCCCCTGGGCACCCTGGGCTACTGTGGGGC
  TGTGGGCCTGTTACCCCCAGGCGCAGCAGCTCCCTCCGGCTGGGCCCAGGCTCCACTCAGTGACACGGCT
  CAAGTCTACATGGAGCTGCAGGGCCTGGTGGACCCGCAGATCCAGCTACCTCTGTTAGCCGCCCGAAGGT
  ACAAGTTGCAGAAGCAGCTTGACAGCCTCACAGCCAGGACCCCATCAGAAGGGGAGGCAGGGACTCAGAG
  GCAACAAAAGCTTTCTTCCCTCCAGCTGGAATTGTCAAAACTGGACAAGGCAGCCTCTCACCTCCGGCAG
  CTGATGGATGAGCCTCCAGCCCCAGGGAGCCCGGAGCTC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG230585 representing NM_001167733
  Red=Cloning site Green=Tags(s)
  
  MCIPPPNVTGSLHIGHALTVAIQDALVRWHRMRGDQVLWVPGSDHAGIATQAVVEKQLWKERGVRRHELS
  REAFLREVWQWKEAKGGEICEQLRALGASLDWDRECFTMDVGSSVAVTEAFVRLYKAGLLYRNHQLVNWS
  CALRSAISDIEVENRPLPGHTQLRLPGCPTPVSFGLLFSVAFPVDGEPDAEVVVGTTRPETLPGDVAVAV
  HPDDSRYTHLHGRQLRHPLMGQPLPLITDYAVQPHVGTGAVKVTPAHSPADAEMGARHGLSPLNVIAEDG
  TMTSLCGDWLQGLHRFVAREKIMSVLSEWGLFRGLQNHPMVLPICSRSGDVIEYLLKNQWFVRCQEMGAR
  AAKAVESGALELSPSFHQKNWQHWFSHIGDWCVSRQLWWGHQIPAYLVVEDHAQGEEDCWVVGRSEAEAR
  EVAAELTGRPGAELTLERDPDVLDTWFSSALFPFSALGWPQETPDLARFYPLSLLETGSDLLLFWVGRMV
  MLGTQLTGQLPFSKVLLHPMVRDRQGRKMSKSLGNVLDPRDIISGVEMQVLQEKLRSGNLDPAELAIVAA
  AQKKDFPHGIPECGTDALRFTLCSHGVQAGDLHLSVSEVQSCRHFCNKIWNALRFILNALGEKFVPQPAE
  ELSPSSPMDAWILSRLALAAQECERGFLTRELSLVTHALHHFWLHNLCDVYLEAVKPVLWHSPRPLGPPQ
  VLFSCADLGLRLLAPLMPFLAEELWQRLPPRPGCPPAPSISVAPYPSACSLEHWRQPELERRFSRVQEVV
  QVLRALRATYQLTKARPRVLLQSSEPGDQGLFEAFLEPLGTLGYCGAVGLLPPGAAAPSGWAQAPLSDTA
  QVYMELQGLVDPQIQLPLLAARRYKLQKQLDSLTARTPSEGEAGTQRQQKLSSLQLELSKLDKAASHLRQ
  LMDEPPAPGSPEL
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001167733
• ORF Size: 2769 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_001167733.1, NP_001161205.1
• RefSeq Size: 3397
• RefSeq ORF: 2772
• Locus ID: 57176
• Protein Families: Druggable Genome, Transcription Factors
• Protein Pathways: Aminoacyl-tRNA biosynthesis, Valine, leucine and isoleucine biosynthesis
• Gene Summary: This gene encodes a mitochondrial aminoacyl-tRNA synthetase, which catalyzes the attachment of valine to tRNA(Val) for mitochondrial translation. Mutations in this gene cause combined oxidative phosphorylation deficiency-20, and are also associated with early-onset mitochondrial encephalopathies. Alternative splicing of this gene results in multiple transcript variants. [provided by RefSeq, Aug 2014]