PHKA1 (NM_001172436) Human Tagged ORF Clone

CAT#: RG230663

PHKA1 (GFP-tagged) - Human phosphorylase kinase, alpha 1 (muscle) (PHKA1), transcript variant 3

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: PHKA1
• Synonyms: PHKA
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG230663 representing NM_001172436
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGGAGCCGGAGTAACTCCGGGGTCCGGCTGGACGGCTACGCTCGACTGGTGCAACAGACCATCCTGT
  GCCATCAGAATCCAGTGACTGGCTTGCTTCCAGCCAGCTATGATCAGAAAGATGCTTGGGTCCGAGATAA
  TGTGTACAGCATCTTGGCTGTGTGGGGTTTGGGCCTGGCCTATCGGAAGAATGCAGACCGGGATGAGGAT
  AAGGCAAAGGCCTATGAATTGGAGCAGAGTGTAGTGAAGCTGATGAGAGGACTACTGCACTGCATGATCA
  GACAGGTGGATAAAGTAGAATCCTTCAAATATAGTCAGAGTACTAAGGATAGCCTCCATGCAAAGTACAA
  CACCAAAACCTGTGCCACTGTAGTGGGTGATGATCAATGGGGACACCTGCAGTTGGATGCTACCTCTGTG
  TACCTGCTCTTCTTAGCCCAAATGACTGCCTCAGGACTCCATATCATCCACAGCCTAGATGAAGTCAATT
  TCATACAGAACCTTGTGTTTTACATTGAAGCTGCATATAAAACTGCTGACTTCGGGATATGGGAACGTGG
  AGACAAGACCAACCAAGGGATCTCAGAGTTGAATGCCAGTTCAGTTGGAATGGCAAAGGCAGCCCTGGAA
  GCATTAGATGAACTGGATCTGTTTGGTGTGAAAGGTGGGCCTCAATCAGTTATCCATGTCCTGGCTGATG
  AAGTACAGCACTGCCAGTCTATCCTAAATTCACTACTGCCCCGTGCTTCAACATCAAAAGAGGTTGATGC
  TAGTCTACTCTCAGTGGTTTCCTTCCCTGCCTTTGCAGTAGAGGATAGCCAGTTGGTGGAGCTCACAAAA
  CAGGAAATCATCACCAAGCTTCAGGGTCGTTATGGTTGCTGTCGCTTTCTACGAGATGGATATAAAACTC
  CTAAAGAGGATCCCAATCGTCTGTACTATGAACCAGCTGAGCTGAAGCTATTTGAAAACATTGAGTGTGA
  ATGGCCATTGTTCTGGACATACTTTATTCTTGATGGGGTCTTCAGTGGCAATGCAGAACAGGTTCAAGAA
  TATAAAGAGGCTCTTGAAGCAGTCCTCATCAAGGGCAAAAATGGAGTCCCACTTCTGCCAGAGCTGTACA
  GTGTTCCTCCTGACAGGGTCGATGAAGAATATCAGAATCCTCACACTGTGGACCGAGTCCCCATGGGGAA
  ATTGCCTCACATGTGGGGTCAGTCTCTATACATTTTAGGAAGCTTGATGGCAGAGGGATTTTTAGCCCCT
  GGAGAAATTGATCCCCTGAATCGCAGGTTTTCTACTGTACCGAAGCCCGATGTTGTGGTTCAAGTCTCCA
  TTCTAGCTGAAACAGAAGAAATCAAGACCATTTTGAAGGACAAGGGAATTTACGTGGAGACCATTGCTGA
  GGTATACCCCATCAGAGTACAACCAGCTCGTATTCTCAGCCACATTTATTCCAGCCTAGGATGCAACAAT
  AGAATGAAACTCAGTGGACGACCCTACAGACACATGGGAGTGCTTGGAACTTCAAAACTCTATGACATTC
  GGAAAACTATCTTTACTTTCACTCCACAGTTTATAGACCAGCAACAGTTCTACCTGGCTCTGGACAACAA
  GATGATAGTGGAAATGCTTAGAACAGACCTCTCCTACCTCTGTAGCCGCTGGCGGATGACAGGCCAGCCC
  ACCATCACCTTCCCCATCTCACACAGCATGCTTGATGAAGATGGAACAAGCTTGAATTCAAGTATCCTGG
  CAGCACTCCGAAAAATGCAAGATGGGTATTTTGGTGGGGCAAGGGTTCAAACAGGTAAATTGTCAGAGTT
  TTTGACAACATCTTGTTGCACACACTTGAGCTTCATGGACCCTGGACCTGAGGGTAAGCTGTACAGTGAA
  GATTATGATGACAACTATGATTACCTGGAATCTGGCAACTGGATGAATGATTATGATTCAACCAGTCATG
  ATGTTCACATGTATCTTCCTACGAAGTTATTTCAGGCTTCCCGGCCTTCATTCAACTTACTTGATTCACC
  TCATCCCCGACAGGAGAACCAGGTTCCCTCTGTTCGTGTAGAAATACATCTTCCTAGAGACCAGTCTGGG
  GAGGTGGACTTTAAAGCACTGGTTTTACAGTTGAAGGAGACCTCAAGCTTACAGGAACAAGCTGATATCC
  TCTATATGCTGTATACTATGAAAGGACCTGACTGGAACACTGAATTGTATAATGAACGGAGTGCTACAGT
  GAGAGAGCTTCTTACCGAGCTGTATGGCAAAGTGGGAGAAATTCGTCACTGGGGCCTGATCCGATACATT
  TCTGGGATCTTAAGGAAGAAAGTGGAAGCACTTGATGAGGCCTGCACAGACCTTCTCTCCCACCAGAAAC
  ATTTGACAGTAGGACTTCCTCCAGAACCTCGAGAAAAGACTATCTCTGCACCTCTGCCCTATGAGGCGCT
  CACTCAGCTGATAGATGAAGCCAGTGAAGGGGATATGAGCATTTCAATCCTTACACAGGAAATAATGGTA
  TATCTAGCCATGTATATGCGAACCCAGCCTGGCCTCTTTGCTGAAATGTTTCGACTTCGAATTGGTCTGA
  TCATACAAGTTATGGCAACAGAACTGGCCCACTCCCTTCGATGCTCAGCTGAGGAAGCCACAGAGGGCCT
  GATGAATCTCAGTCCTTCGGCCATGAAGAATCTCCTGCATCACATTCTCAGCGGCAAGGAGTTTGGAGTG
  GAACGAAGCGTTCGTCCCACTGATTCAAATGTCAGTCCTGCTATTTCTATCCACGAGATTGGTGCTGTTG
  GAGCAACCAAAACAGAACGAACTGGGATCATGCAGTTAAAAAGTGAGATAAAGCAGTCACCTGGAACCTC
  TATGACTCCAAGTAGTGGGTCCTTTCCTAGTGCATATGATCAGCAGTCATCTAAAGATAGTCGTCAAGGT
  CAATGGCAACGCCGAAGAAGGCTGGATGGGGCACTGAATAGAGTTCCAGTTGGATTTTATCAGAAAGTAT
  GGAAAGTTTTGCAGAAGTGTCACGGACTTTCTGTTGAAGGGTTTGTCCTTCCTTCCTCTACCACTAGAGA
  GATGACTCCAGGTGAGATTAAATTCTCTGTTCATGTGGAGTCTGTCCTGAATCGTGTACCTCAGCCAGAG
  TACCGTCAGCTGCTGGTTGAAGCCATCCTTGTCCTCACCATGCTGGCAGATATTGAAATTCATAGCATCG
  GAAGCATCATTGCTGTGGAAAAAATAGTGCATATTGCCAATGACTTGTTCCTTCAAGAACAGAAAACCCT
  TGGCGCAGATGATACCATGTTGGCAAAGGATCCCGCATCTGGCATCTGTACTCTTCTGTATGACAGTGCA
  CCCAGTGGCAGGTTTGGCACCATGACCTACCTCTCCAAGGCAGCCGCCACCTACGTGCAGGAGTTCCTGC
  CCCACAGCATCTGTGCCATGCAA
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG230663 representing NM_001172436
  Red=Cloning site Green=Tags(s)
  
  MRSRSNSGVRLDGYARLVQQTILCHQNPVTGLLPASYDQKDAWVRDNVYSILAVWGLGLAYRKNADRDED
  KAKAYELEQSVVKLMRGLLHCMIRQVDKVESFKYSQSTKDSLHAKYNTKTCATVVGDDQWGHLQLDATSV
  YLLFLAQMTASGLHIIHSLDEVNFIQNLVFYIEAAYKTADFGIWERGDKTNQGISELNASSVGMAKAALE
  ALDELDLFGVKGGPQSVIHVLADEVQHCQSILNSLLPRASTSKEVDASLLSVVSFPAFAVEDSQLVELTK
  QEIITKLQGRYGCCRFLRDGYKTPKEDPNRLYYEPAELKLFENIECEWPLFWTYFILDGVFSGNAEQVQE
  YKEALEAVLIKGKNGVPLLPELYSVPPDRVDEEYQNPHTVDRVPMGKLPHMWGQSLYILGSLMAEGFLAP
  GEIDPLNRRFSTVPKPDVVVQVSILAETEEIKTILKDKGIYVETIAEVYPIRVQPARILSHIYSSLGCNN
  RMKLSGRPYRHMGVLGTSKLYDIRKTIFTFTPQFIDQQQFYLALDNKMIVEMLRTDLSYLCSRWRMTGQP
  TITFPISHSMLDEDGTSLNSSILAALRKMQDGYFGGARVQTGKLSEFLTTSCCTHLSFMDPGPEGKLYSE
  DYDDNYDYLESGNWMNDYDSTSHDVHMYLPTKLFQASRPSFNLLDSPHPRQENQVPSVRVEIHLPRDQSG
  EVDFKALVLQLKETSSLQEQADILYMLYTMKGPDWNTELYNERSATVRELLTELYGKVGEIRHWGLIRYI
  SGILRKKVEALDEACTDLLSHQKHLTVGLPPEPREKTISAPLPYEALTQLIDEASEGDMSISILTQEIMV
  YLAMYMRTQPGLFAEMFRLRIGLIIQVMATELAHSLRCSAEEATEGLMNLSPSAMKNLLHHILSGKEFGV
  ERSVRPTDSNVSPAISIHEIGAVGATKTERTGIMQLKSEIKQSPGTSMTPSSGSFPSAYDQQSSKDSRQG
  QWQRRRRLDGALNRVPVGFYQKVWKVLQKCHGLSVEGFVLPSSTTREMTPGEIKFSVHVESVLNRVPQPE
  YRQLLVEAILVLTMLADIEIHSIGSIIAVEKIVHIANDLFLQEQKTLGADDTMLAKDPASGICTLLYDSA
  PSGRFGTMTYLSKAAATYVQEFLPHSICAMQ
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001172436
• ORF Size: 3453 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001172436.1, NP_001165907.1
• RefSeq Size: 5961 bp
• RefSeq ORF: 3456 bp
• Locus ID: 5255
• Cytogenetics: Xq13.1
• Protein Families: Druggable Genome
• Protein Pathways: Calcium signaling pathway, Insulin signaling pathway
• Gene Summary: 'Phosphorylase kinase is a polymer of 16 subunits, four each of alpha, beta, gamma and delta. The alpha subunit includes the skeletal muscle and hepatic isoforms, and the skeletal muscle isoform is encoded by this gene. The beta subunit is the same in both the muscle and hepatic isoforms, and encoded by one gene. The gamma subunit also includes the skeletal muscle and hepatic isoforms, which are encoded by two different genes. The delta subunit is a calmodulin and can be encoded by three different genes. The gamma subunits contain the active site of the enzyme, whereas the alpha and beta subunits have regulatory functions controlled by phosphorylation. The delta subunit mediates the dependence of the enzyme on calcium concentration. Mutations in this gene cause glycogen storage disease type 9D, also known as X-linked muscle glycogenosis. Alternatively spliced transcript variants encoding different isoforms have been identified in this gene. A pseudogene has been found on chromosome 1.[provided by RefSeq, Feb 2010]'