AFF2 (NM_001169125) Human Tagged ORF Clone

CAT#: RG230688

AFF2 (GFP-tagged) - Human AF4/FMR2 family, member 2 (AFF2), transcript variant 5

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: AFF2
• Synonyms: FMR2; FMR2P; FRAXE; MRX2; OX19
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG230688 representing NM_001169125
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGATCTATTCGACTTTTTCAGAGACTGGGACTTGGAGCAGCAGTGTCACTATGAACAAGACCGTAGTG
  CACTTAAAAAAAGGGAATGGGAGCGGAGGAATCAAGAAGTCCAGCAAGAAGACGATCTCTTTTCTTCAGG
  CTTTGATCTTTTTGGGGAGCCATACAAGACAAACAAAGGTGATGCACTTGCCAACCGAGTCCAGAACACG
  CTTGGAAACTATGATGAAATGAAGAATTTGCTAACTAACCATTCTAATCAGAATCACCTAGTGGGAATTC
  CAAAGAATTCTGTGCCCCAGAATCCCAACAACAAAAATGAACCAAGCTTTTTTCCAGAACAAAAGAACAG
  AATAATTCCACCTCACCAGGATAATACCCATCCTTCAGCACCAATGCCTCCACCTTCTGTTGTGATACTG
  AATTCAACTCTAATACACAGCAACAGAAAATCAAAACCTGAGTGGTCACGTGATAGTCATAACCCTAGCA
  CTGTACTGGCAAGCCAGGCCAGTGGTCAGCCAAACAAGATGCAGACTTTGACACAGGACCAGTCTCAAGC
  CAAACTGGAAGACTTCTTTGTCTACCCAGCTGAACAGCCCCAGATTGGAGAAGTTGAAGAGTCAAACCCA
  TCTGCAAAGGAAGACAGTAACCCTAATTCTAGTGGAGAAGATGCTTTCAAAGAAATCTTTCAATCCAATT
  CACCGGAAGAATCTGAATTCGCCGTGCAAGCGCCTGGGTCTCCCCTAGTGGCTTCCTCTTTATTAGCTCC
  TAGCAGTGGCCTTTCAGTTCAAAACTTCCCACCAGGGCTTTACTGCAAAACAAGCATGGGGCAGCAAAAG
  CCAACTGCATACGTCAGACCCATGGATGGCCAGGACCAGGCACCGGACATCTCACCAACACTGAAACCTT
  CAATTGAATTTGAGAACAGCTTTGGGAATCTGTCATTTGGAACACTCTTGGATGGAAAACCCAGTGCAGC
  CAGTTCAAAGACTAAACTGCCAAAGTTCACCATCCTCCAAACAAGTGAAGTAAGCCTTCCCAGTGATCCA
  AGCTGTGTTGAAGAAATCTTGCGGGAATCGCAGCATCTGACCCCAGGATTCACCTTACAAAAGTGGAATG
  ACCCAACCACCAGAGCTTCTACAAAGATGCTTGAGGATGACCTGAAGCTGAGCAGTGATGAAGATGACCT
  TGAGCCTGTGAAGACCTTGACCACTCAGTGCACTGCCACTGAGCTCTACCAGGCTGTTGAAAAGGCAAAA
  CCTAGGAATAATCCTGTGAACCCACCCTTGGCCACTCCCCAGCCCCCACCTGCAGTGCAAGCCAGCGGGG
  GTTCTGGCAGCTCCAGCGAATCGGAGAGCAGCTCTGAGTCGGATTCAGACACTGAAAGTAGCACCACTGA
  CAGCGAATCTAATGAGGCACCTCGTGTGGCAACTCCAGAGCCTGAGCCACCCTCAACCAACAAGTGGCAA
  CTGGATAAATGGCTTAACAAAGTGACATCCCAGAACAAGTCTTTTATTTGTGGCCAAAATGAAACACCCA
  TGGAGACTATTTCTCTGCCTCCTCCAATCATCCAACCAATGGAAGTCCAGATGAAAGTGAAGACGAATGC
  CAGTCAGGTCCCAGCTGAACCCAAAGAAAGGCCTCTCCTCAGTCTCATTAGGGAGAAAGCCCGTCCACGG
  CCCACTCAGAAAATTCCAGAAACAAAGGCTTTGAAGCATAAGTTGTCAACAACTAGTGAGACAGTGTCTC
  AAAGGACAATTGGGAAAAAACAGCCCAAAAAAGTTGAGAAGAACACCAGCACTGACGAGTTTACCTGGCC
  CAAACCAAATATTACCAGCAGCACTCCCAAAGAAAAAGAAAGTGTGGAGCTTCATGACCCACCAAGAGGC
  CGCAACAAAGCCACTGCCCACAAACCAGCCCCTAGGAAAGAACCAAGACCTAACATCCCTTTGGCTCCCG
  AGAAGAAGAAGTACAGAGGGCCTGGCAAGATTGTGCCAAAGTCTCGGGAATTCATTGAAACAGATTCATC
  TACATCTGACTCCAACACAGATCAGGAAGAGACCCTGCAAATCAAAGTCCTGCCTCCGTGCATTATTTCT
  GGAGGTAATACTGCCAAATCCAAGGAAATCTGTGGTGCCAGCCTGACCCTCAGCACCTTAATGAGTAGCA
  GTGGCAGCAACAACAACTTATCCATCAGTAATGAAGAGCCAACATTTTCACCTATTCCTGTCATGCAAAC
  TGAAATCCTGTCCCCTCTGCGAGATCATGAGAACCTGAAAAACCTCTGGGTGAAGATTGACCTTGACTTA
  CTCTCTAGAGTACCTGGCCACAGCTCACTCCATGCAGCACCTGCCAAGCCAGACCACAAGGAGACTGCCA
  CAAAACCCAAGCGTCAGACAGCTGTCACAGCTGTGGAGAAACCAGCCCCTAAGGGCAAACGTAAGCACAA
  GCCAATAGAAGTTGCAGAGAAGATCCCTGAGAAGAAGCAGCGCCTGGAGGAGGCCACAACTATCTGCTTG
  CTCCCTCCTTGCATCTCACCAGCCCCACCCCACAAGCCTCCCAACACTAGAGAAAATAATTCATCCAGGA
  GAGCAAATAGAAGAAAGGAAGAAAAACTATTTCCTCCTCCACTTTCCCCACTGCCAGAGGACCCTCCACG
  CCGCAGAAATGTCAGTGGCAATAATGGTCCCTTTGGTCAAGACAAAAACATCGCCATGACTGGACAAATC
  ACATCTACCAAACCTAAGAGAACTGAAGGCAAATTCTGTGCTACTTTCAAAGGGATATCGGTAAATGAGG
  GAGACACTCCAAAAAAGGCATCCTCTGCCACCATCACTGTCACCAATACTGCTATTGCCACTGCTACTGT
  CACTGCTACTGCCATTGTCACCACCACTGTCACAGCTACTGCCACCGCCACGGCCACCACCACAACTACT
  ACCACTACCATTTCCACCATCACCTCTACCATCACTACTGGCCTCATGGATAGCAGTCACCTGGAGATGA
  CGTCCTGGGCGGCTCTGCCCCTTCTATCCAGCAGCAGCACTAATGTCCGGAGACCCAAGCTCACTTTTGA
  TGACTCGGTTCACAATGCTGATTATTACATGCAAGAAGCTAAGAAGCTGAAGCACAAAGCTGATGCACTG
  TTCGAGAAATTTGGCAAAGCTGTGAATTATGCTGATGCCGCCCTCTCCTTCACTGAATGTGGCAATGCCA
  TGGAACGCGACCCTCTGGAAGCAAAGTCCCCATACACCATGTACTCTGAGACTGTGGAGCTCCTCAGGTA
  TGCAATGAGGCTGAAGAACTTTGCAAGTCCCTTGGCTTCGGATGGGGACAAAAAGCTAGCAGTACTATGC
  TACCGATGTTTATCACTCCTCTATTTGAGAATGTTTAAGCTGAAGAAGGACCATGCTATGAAGTACTCCA
  GATCACTGATGGAATATTTTAAGCAAAATGCTTCAAAAGTCGCACAGATACCCTCTCCATGGGTAAGCAA
  TGGAAAGAACACTCCATCCCCAGTGTCTCTCAACAACGTCTCCCCCATCAACGCAATGGGGAACTGTAAC
  AATGGCCCAGTCACCATTCCCCAGCGCATTCACCACATGGCTGCCAGCCACGTCAACATCACTAGCAATG
  TGTTACGGGGCTATGAACACTGGGATATGGCCGACAAACTGACAAGAGAAAACAAAGAATTCTTTGGTGA
  TCTGGACACGCTGATGGGGCCTCTGACCCAGCACAGCAGCATGACCAATCTTGTCCGCTACGTTCGCCAA
  GGACTGTGTTGGCTGCGCATCGATGCCCACTTGTTG
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG230688 representing NM_001169125
  Red=Cloning site Green=Tags(s)
  
  MDLFDFFRDWDLEQQCHYEQDRSALKKREWERRNQEVQQEDDLFSSGFDLFGEPYKTNKGDALANRVQNT
  LGNYDEMKNLLTNHSNQNHLVGIPKNSVPQNPNNKNEPSFFPEQKNRIIPPHQDNTHPSAPMPPPSVVIL
  NSTLIHSNRKSKPEWSRDSHNPSTVLASQASGQPNKMQTLTQDQSQAKLEDFFVYPAEQPQIGEVEESNP
  SAKEDSNPNSSGEDAFKEIFQSNSPEESEFAVQAPGSPLVASSLLAPSSGLSVQNFPPGLYCKTSMGQQK
  PTAYVRPMDGQDQAPDISPTLKPSIEFENSFGNLSFGTLLDGKPSAASSKTKLPKFTILQTSEVSLPSDP
  SCVEEILRESQHLTPGFTLQKWNDPTTRASTKMLEDDLKLSSDEDDLEPVKTLTTQCTATELYQAVEKAK
  PRNNPVNPPLATPQPPPAVQASGGSGSSSESESSSESDSDTESSTTDSESNEAPRVATPEPEPPSTNKWQ
  LDKWLNKVTSQNKSFICGQNETPMETISLPPPIIQPMEVQMKVKTNASQVPAEPKERPLLSLIREKARPR
  PTQKIPETKALKHKLSTTSETVSQRTIGKKQPKKVEKNTSTDEFTWPKPNITSSTPKEKESVELHDPPRG
  RNKATAHKPAPRKEPRPNIPLAPEKKKYRGPGKIVPKSREFIETDSSTSDSNTDQEETLQIKVLPPCIIS
  GGNTAKSKEICGASLTLSTLMSSSGSNNNLSISNEEPTFSPIPVMQTEILSPLRDHENLKNLWVKIDLDL
  LSRVPGHSSLHAAPAKPDHKETATKPKRQTAVTAVEKPAPKGKRKHKPIEVAEKIPEKKQRLEEATTICL
  LPPCISPAPPHKPPNTRENNSSRRANRRKEEKLFPPPLSPLPEDPPRRRNVSGNNGPFGQDKNIAMTGQI
  TSTKPKRTEGKFCATFKGISVNEGDTPKKASSATITVTNTAIATATVTATAIVTTTVTATATATATTTTT
  TTTISTITSTITTGLMDSSHLEMTSWAALPLLSSSSTNVRRPKLTFDDSVHNADYYMQEAKKLKHKADAL
  FEKFGKAVNYADAALSFTECGNAMERDPLEAKSPYTMYSETVELLRYAMRLKNFASPLASDGDKKLAVLC
  YRCLSLLYLRMFKLKKDHAMKYSRSLMEYFKQNASKVAQIPSPWVSNGKNTPSPVSLNNVSPINAMGNCN
  NGPVTIPQRIHHMAASHVNITSNVLRGYEHWDMADKLTRENKEFFGDLDTLMGPLTQHSSMTNLVRYVRQ
  GLCWLRIDAHLL
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001169125
• ORF Size: 3816 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001169125.1, NP_001162596.1
• RefSeq Size: 13629 bp
• RefSeq ORF: 3819 bp
• Locus ID: 2334
• Cytogenetics: Xq28
• Protein Families: Druggable Genome
• Gene Summary: 'This gene encodes a putative transcriptional activator that is a member of the AF4\FMR2 gene family. This gene is associated with the folate-sensitive fragile X E locus on chromosome X. A repeat polymorphism in the fragile X E locus results in silencing of this gene causing Fragile X E syndrome. Fragile X E syndrome is a form of nonsyndromic X-linked cognitive disability. In addition, this gene contains 6-25 GCC repeats that are expanded to >200 repeats in the disease state. Alternate splicing results in multiple transcript variants.[provided by RefSeq, Jul 2016]'