AFF2 (NM_001169122) Human Tagged ORF Clone

CAT#: RG230691

AFF2 (GFP-tagged) - Human AF4/FMR2 family, member 2 (AFF2), transcript variant 2

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: AFF2
• Synonyms: FMR2; FMR2P; FRAXE; MRX2; OX19
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG230691 representing NM_001169122
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGATCTATTCGACTTTTTCAGAGACTGGGACTTGGAGCAGCAGTGTCACTATGAACAAGACCGTAGTG
  CACTTAAAAAAAGGGAATGGGAGCGGAGGAATCAAGAAGTCCAGCAAGAAGACGATCTCTTTTCTTCAGG
  CTTTGATCTTTTTGGGGAGCCATACAAGACAAACAAAGGTGATGCACTTGCCAACCGAGTCCAGAACACG
  CTTGGAAACTATGATGAAATGAAGAATTTGCTAACTAACCATTCTAATCAGAATCACCTAGTGGGAATTC
  CAAAGAATTCTGTGCCCCAGAATCCCAACAACAAAAATGAACCAAGCTTTTTTCCAGAACAAAAGAACAG
  AATAATTCCACCTCACCAGGATAATACCCATCCTTCAGCACCAATGCCTCCACCTTCTGTTGTGATACTG
  AATTCAACTCTAATACACAGCAACAGAAAATCAAAACCTGAGTGGTCACGTGATAGTCATAACCCTAGCA
  CTGTACTGGCAAGCCAGGCCAGTGGTCAGCCAAACAAGATGCAGACTTTGACACAGGACCAGTCTCAAGC
  CAAACTGGAAGACTTCTTTGTCTACCCAGCTGAACAGCCCCAGATTGGAGAAGTTGAAGAGTCAAACCCA
  TCTGCAAAGGAAGACAGTAACCCTAATTCTAGTGGAGAAGATGCTTTCAAAGAAATCTTTCAATCCAATT
  CACCGGAAGAATCTGAATTCGCCGTGCAAGCGCCTGGGTCTCCCCTAGTGGCTTCCTCTTTATTAGCTCC
  TAGCAGTGGCCTTTCAGTTCAAAACTTCCCACCAGGGCTTTACTGCAAAACAAGCATGGGGCAGCAAAAG
  CCAACTGCATACGTCAGACCCATGGATGGCCAGGACCAGGCACCGGACATCTCACCAACACTGAAACCTT
  CAATTGAATTTGAGAACAGCTTTGGGAATCTGTCATTTGGAACACTCTTGGATGGAAAACCCAGTGCAGC
  CAGTTCAAAGACTAAACTGCCAAAGTTCACCATCCTCCAAACAAGTGAAGTAAGCCTTCCCAGTGATCCA
  AGCTGTGTTGAAGAAATCTTGCGGGAATCGCAGCATCTGACCCCAGGATTCACCTTACAAAAGTGGAATG
  ACCCAACCACCAGAGCTTCTACAAAGTCAGTGTCTTTCAAATCGATGCTTGAGGATGACCTGAAGCTGAG
  CAGTGATGAAGATGACCTTGAGCCTGTGAAGACCTTGACCACTCAGTGCACTGCCACTGAGCTCTACCAG
  GCTGTTGAAAAGGCAAAACCTAGGAATAATCCTGTGAACCCACCCTTGGCCACTCCCCAGCCCCCACCTG
  CAGTGCAAGCCAGCGGGGGTTCTGGCAGCTCCAGCGAATCGGAGAGCAGCTCTGAGTCGGATTCAGACAC
  TGAAAGTAGCACCACTGACAGCGAATCTAATGAGGCACCTCGTGTGGCAACTCCAGAGCCTGAGCCACCC
  TCAACCAACAAGTGGCAACTGGATAAATGGCTTAACAAAGTGACATCCCAGAACAAGTCTTTTATTTGTG
  GCCAAAATGAAACACCCATGGAGACTATTTCTCTGCCTCCTCCAATCATCCAACCAATGGAAGTCCAGAT
  GAAAGTGAAGACGAATGCCAGTCAGGTCCCAGCTGAACCCAAAGAAAGGCCTCTCCTCAGTCTCATTAGG
  GAGAAAGCCCGTCCACGGCCCACTCAGAAAATTCCAGAAACAAAGGCTTTGAAGCATAAGTTGTCAACAA
  CTAGTGAGACAGTGTCTCAAAGGACAATTGGGAAAAAACAGCCCAAAAAAGTTGAGAAGAACACCAGCAC
  TGACGAGTTTACCTGGCCCAAACCAAATATTACCAGCAGCACTCCCAAAGAAAAAGAAAGTGTGGAGCTT
  CATGACCCACCAAGAGGCCGCAACAAAGCCACTGCCCACAAACCAGCCCCTAGGAAAGAACCAAGACCTA
  ACATCCCTTTGGCTCCCGAGAAGAAGAAGTACAGAGGGCCTGGCAAGATTGTGCCAAAGTCTCGGGAATT
  CATTGAAACAGATTCATCTACATCTGACTCCAACACAGATCAGGAAGAGACCCTGCAAATCAAAGTCCTG
  CCTCCGTGCATTATTTCTGGAGGTAATACTGCCAAATCCAAGGAAATCTGTGGTGCCAGCCTGACCCTCA
  GCACCTTAATGAGTAGCAGTGGCAGCAACAACAACTTATCCATCAGTAATGAAGAGCCAACATTTTCACC
  TATTCCTGTCATGCAAACTGAAATCCTGTCCCCTCTGCGAGATCATGAGAACCTGAAAAACCTCTGGGTG
  AAGATTGACCTTGACTTACTCTCTAGAGTACCTGGCCACAGCTCACTCCATGCAGCACCTGCCAAGCCAG
  ACCACAAGGAGACTGCCACAAAACCCAAGCGTCAGACAGCTGTCACAGCTGTGGAGAAACCAGCCCCTAA
  GGGCAAACGTAAGCACAAGCCAATAGAAGTTGCAGAGAAGATCCCTGAGAAGAAGCAGCGCCTGGAGGAG
  GCCACAACTATCTGCTTGCTCCCTCCTTGCATCTCACCAGCCCCACCCCACAAGCCTCCCAACACTAGAG
  AAAATAATTCATCCAGGAGAGCAAATAGAAGAAAGGAAGAAAAACTATTTCCTCCTCCACTTTCCCCACT
  GCCAGAGGACCCTCCACGCCGCAGAAATGTCAGTGGCAATAATGGTCCCTTTGGTCAAGACAAAAACATC
  GCCATGACTGGACAAATCACATCTACCAAACCTAAGAGAACTGAAGGCAAATTCTGTGCTACTTTCAAAG
  GGATATCGGAGGGAGACACTCCAAAAAAGGCATCCTCTGCCACCATCACTGTCACCAATACTGCTATTGC
  CACTGCTACTGTCACTGCTACTGCCATTGTCACCACCACTGTCACAGCTACTGCCACCGCCACGGCCACC
  ACCACAACTACTACCACTACCATTTCCACCATCACCTCTACCATCACTACTGGCCTCATGGATAGCAGTC
  ACCTGGAGATGACGTCCTGGGCGGCTCTGCCCCTTCTATCCAGCAGCAGCACTAATGTCCGGAGACCCAA
  GCTCACTTTTGATGACTCGGTTCACAATGCTGATTATTACATGCAAGAAGCTAAGAAGCTGAAGCACAAA
  GCTGATGCACTGTTCGAGAAATTTGGCAAAGCTGTGAATTATGCTGATGCCGCCCTCTCCTTCACTGAAT
  GTGGCAATGCCATGGAACGCGACCCTCTGGAAGCAAAGTCCCCATACACCATGTACTCTGAGACTGTGGA
  GCTCCTCAGGTATGCAATGAGGCTGAAGAACTTTGCAAGTCCCTTGGCTTCGGATGGGGACAAAAAGCTA
  GCAGTACTATGCTACCGATGTTTATCACTCCTCTATTTGAGAATGTTTAAGCTGAAGAAGGACCATGCTA
  TGAAGTACTCCAGATCACTGATGGAATATTTTAAGCAAAATGCTTCAAAAGTCGCACAGATACCCTCTCC
  ATGGGTAAGCAATGGAAAGAACACTCCATCCCCAGTGTCTCTCAACAACGTCTCCCCCATCAACGCAATG
  GGGAACTGTAACAATGGCCCAGTCACCATTCCCCAGCGCATTCACCACATGGCTGCCAGCCACGTCAACA
  TCACTAGCAATGTGTTACGGGGCTATGAACACTGGGATATGGCCGACAAACTGACAAGAGAAAACAAAGA
  ATTCTTTGGTGATCTGGACACGCTGATGGGGCCTCTGACCCAGCACAGCAGCATGACCAATCTTGTCCGC
  TACGTTCGCCAAGGACTGTGTTGGCTGCGCATCGATGCCCACTTGTTG
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG230691 representing NM_001169122
  Red=Cloning site Green=Tags(s)
  
  MDLFDFFRDWDLEQQCHYEQDRSALKKREWERRNQEVQQEDDLFSSGFDLFGEPYKTNKGDALANRVQNT
  LGNYDEMKNLLTNHSNQNHLVGIPKNSVPQNPNNKNEPSFFPEQKNRIIPPHQDNTHPSAPMPPPSVVIL
  NSTLIHSNRKSKPEWSRDSHNPSTVLASQASGQPNKMQTLTQDQSQAKLEDFFVYPAEQPQIGEVEESNP
  SAKEDSNPNSSGEDAFKEIFQSNSPEESEFAVQAPGSPLVASSLLAPSSGLSVQNFPPGLYCKTSMGQQK
  PTAYVRPMDGQDQAPDISPTLKPSIEFENSFGNLSFGTLLDGKPSAASSKTKLPKFTILQTSEVSLPSDP
  SCVEEILRESQHLTPGFTLQKWNDPTTRASTKSVSFKSMLEDDLKLSSDEDDLEPVKTLTTQCTATELYQ
  AVEKAKPRNNPVNPPLATPQPPPAVQASGGSGSSSESESSSESDSDTESSTTDSESNEAPRVATPEPEPP
  STNKWQLDKWLNKVTSQNKSFICGQNETPMETISLPPPIIQPMEVQMKVKTNASQVPAEPKERPLLSLIR
  EKARPRPTQKIPETKALKHKLSTTSETVSQRTIGKKQPKKVEKNTSTDEFTWPKPNITSSTPKEKESVEL
  HDPPRGRNKATAHKPAPRKEPRPNIPLAPEKKKYRGPGKIVPKSREFIETDSSTSDSNTDQEETLQIKVL
  PPCIISGGNTAKSKEICGASLTLSTLMSSSGSNNNLSISNEEPTFSPIPVMQTEILSPLRDHENLKNLWV
  KIDLDLLSRVPGHSSLHAAPAKPDHKETATKPKRQTAVTAVEKPAPKGKRKHKPIEVAEKIPEKKQRLEE
  ATTICLLPPCISPAPPHKPPNTRENNSSRRANRRKEEKLFPPPLSPLPEDPPRRRNVSGNNGPFGQDKNI
  AMTGQITSTKPKRTEGKFCATFKGISEGDTPKKASSATITVTNTAIATATVTATAIVTTTVTATATATAT
  TTTTTTTISTITSTITTGLMDSSHLEMTSWAALPLLSSSSTNVRRPKLTFDDSVHNADYYMQEAKKLKHK
  ADALFEKFGKAVNYADAALSFTECGNAMERDPLEAKSPYTMYSETVELLRYAMRLKNFASPLASDGDKKL
  AVLCYRCLSLLYLRMFKLKKDHAMKYSRSLMEYFKQNASKVAQIPSPWVSNGKNTPSPVSLNNVSPINAM
  GNCNNGPVTIPQRIHHMAASHVNITSNVLRGYEHWDMADKLTRENKEFFGDLDTLMGPLTQHSSMTNLVR
  YVRQGLCWLRIDAHLL
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001169122
• ORF Size: 3828 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001169122.1, NP_001162593.1
• RefSeq Size: 13641 bp
• RefSeq ORF: 3831 bp
• Locus ID: 2334
• Cytogenetics: Xq28
• Protein Families: Druggable Genome
• Gene Summary: 'This gene encodes a putative transcriptional activator that is a member of the AF4\FMR2 gene family. This gene is associated with the folate-sensitive fragile X E locus on chromosome X. A repeat polymorphism in the fragile X E locus results in silencing of this gene causing Fragile X E syndrome. Fragile X E syndrome is a form of nonsyndromic X-linked cognitive disability. In addition, this gene contains 6-25 GCC repeats that are expanded to >200 repeats in the disease state. Alternate splicing results in multiple transcript variants.[provided by RefSeq, Jul 2016]'